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Alkaloids, saponins and phenolic compounds in salt marsh plants from the Lower Saxonian Wadden Sea

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Abstract

Twenty-eight plant species belonging to fifteen families were subjected to a screening for total alkaloids, saponins and phenolic compounds, especially flavonoids and tannins. BothWagner’s andMayer’s reagents were employed in the alkaloid screening. Ferric chloride solution was used to detect phenolic compounds. Tannins were detected by the gelatine-salt block test.Shinoda’s colour reaction was used to detect flavonoids. Saponins were detected by the froth method. The species investigated are characteristic salt marsh plants of the Lower Saxonian Wadden Sea coast. Five species gave positive alkaloid reactions with both reagents while four others were reactive only in one test. For the other twenty-three species the presence of alkaloids can be excluded. Ten species did not contain any phenolic compounds while eighteen showed a colour reaction with ferric chloride solution. Nine of these gave a positive reaction with the gelatine-salt block test and twelve gave positive reactions to the flavonoid test. It can therefore be assumed that at least three species contain phenolic compounds other than flavonoids or tannins. Seven species gave a positive saponin test.

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References

  • Anonymous (1999): WHO informal consultation on clinical neurological investigations required for patients treated with artemisinin compounds and derivatives. — Rep. Inf. Consul. WHO,TDR/TDF/99.1: 1–11.

  • Beckmann, D. &Beckmann, D. (1998): Das geheime Wissen der Kräuterhexen. — 267 pp.; München (Deutscher Taschenbuch Verlag).

    Google Scholar 

  • Bertram, B. (1989): Flavonoide, eine Klasse von Pflanzeninhaltsstoffen mit vielfältigen biologischen Wirkungen, auch mit karzinogener Wirkung? — Dtsch. Apotheker-Ztg.,129: 2561–2571.

    CAS  Google Scholar 

  • Cordell, G. A. (1981): Introduction to Alkaloids. A Biogenetic Approach. — 1055 pp.; New York, Chichester, Brisbane, Toronto (Wiley & Sons).

    Google Scholar 

  • Crawford, D. J. (1978): Flavonoid chemistry and angiosperm evolution. — Bot. Rev.,44: 431–456.

    Article  CAS  Google Scholar 

  • Duncan, A. C. &Jäger, A. K. &Staden, J. van (1999): Screening of Zulu medicinal plants for angiotensin converting enzyme (ACE) inhibitors. — J. Ethnopharmacol.,68: 63–70.

    Article  PubMed  CAS  Google Scholar 

  • Euler, K. L. &Farnsworth, N. R. (1962): A comparison of certain alkaloid screening procedures. — Lloydia,25: 296–311.

    CAS  Google Scholar 

  • Farnsworth, N. R. (1966): Biological and phytochemical screening of plants. — J. pharmaceut. Sci.,55: 225–276.

    Article  CAS  Google Scholar 

  • Farnsworth, N. R. &Pilewski, N. A. &Draus, F. J. (1962): Studies on false-positive alkaloid reaction with Dragendorff’s reagent. — Lloydia,25: 312–319.

    CAS  Google Scholar 

  • Flamm, S. &Kroeber, L. (1936): Die Heilkraft der Pflanzen. — 274 pp.; Stuttgart, Leipzig (Hippokrates-Verlag).

    Google Scholar 

  • Franz, G. &Grün, M. (1983): Chemistry, occurrence and biosynthesis of C-glycosyl compounds in plants. — Planta medica,43: 131–140.

    Article  Google Scholar 

  • Glahn, H. von &Dahmen, R. &Lemm, R. von &Wolff, D. (1989): Vegetationssytematische Untersuchungen und großmaßstäbliche Vegetationskartierungen in den Außengroden der niedersächsischen Nordseeküste. — Drosera,89: 145–168.

    Google Scholar 

  • Gornall, R. J. &Bohm, B. A. &Dahlgren, R. (1979): The distribution of flavonoids in the angiosperms. — Bot. Not.,132: 1–30.

    CAS  Google Scholar 

  • Gottlieb, O. R. &Kaplan, M. A. C. &Kubitzki, K. (1993): A suggested role of galloyl esters in the evolution of dicotyledones. — Taxon,42: 539–552.

    Article  Google Scholar 

  • Gross, D. (1977): Phytoalexine und verwandte Pflanzenstoffe. — Fortschr. Chem. Org. Naturst.,34: 187–247.

    CAS  Google Scholar 

  • Gross, M. (1998): Life on the Edge — Amazing Creatures Thriving in Extreme Environments. — 200 pp.; New York (Plenum Press).

    Google Scholar 

  • Hamon, N. W. &Hindmarsh, K. W. (1978): Phytochemical screening of indigenous Saskatchewan plants: Alkaloids; Part I. — Canad. J. pharmaceut. Sci.,13: 39–42.

    CAS  Google Scholar 

  • Hashimoto, T. &Yamada, Y. (1994): Alkaloid biogenesis: Molecular aspects. — Annu. Rev. Plant Physiol. Plant mol. Biol.,45: 257–285.

    CAS  Google Scholar 

  • Hegnauer, R. (1988): Biochemistry, distribution and taxonomic relevance of higher plant alkaloids. — Phytochemistry,27: 2423–2427.

    Article  CAS  Google Scholar 

  • Hill, R. T. &Murphy, P. (1998): L’océan pharmacien. — Biofutur,1998 (6): 34–37.

    Article  Google Scholar 

  • Hiradate, S. &Yada, H. &Ishii, T. &Nakajima, N. &Ohnishi-Kameyama, M. &Sugie H. &Zungsontiporn, S. &Fujii, Y. (1999): Three plant growth inhibiting saponins fromDuranta repens. — Phytochemistry,52: 1223–1228.

    Article  CAS  Google Scholar 

  • Hollman, P. C. H. &Katan, M. B. (1999): Dietary flavonoids: intake, health effects and bioavailability. — Food and Chem. Toxicol.,37: 937–942.

    Article  CAS  Google Scholar 

  • Houghton, P. J. &Traman, A. (1998): Laboratory Handbook for the Fractionation of Natural Extracts. — 1st Edn.: 199 pp.; London (Chapman & Hall).

    Google Scholar 

  • Hultin, E. &Torsell, K. (1965): Alkaloid screening of Swedish plants. — Phytochemistry,4: 425–433.

    Article  CAS  Google Scholar 

  • Kapoor, L. D. &Kapoor, S. L. &Srivastava, S. N. &Singh, A. &Sharma, P. C. (1971): Survey of Indian plants for saponins, alkaloids and flavonoids. Part II. — Lloydia;34: 94–102.

    PubMed  CAS  Google Scholar 

  • Karrer, W. (1976). Konstitution und Vorkommen der organischen Pflanzenstoffe. — 1205 pp.; Basel (Birkhäuser Verlag).

    Google Scholar 

  • Karrer, W. &Cherbuliez, E. &Eugster, C. H. (1977). Konstitution und Vorkommen der organischen Pflanzenstoffe. Suppl. 1. — 1038 pp.; Basel (Birkhäuser Verlag).

    Google Scholar 

  • Karrer, W. &Hürlimann, H. &Cherbuliez, E. (1981). Konstitution und Vorkommen der organischen Pflanzenstoffe. Suppl. 2, Pt. 1. — 939 pp.; Basel (Birkhäuser Verlag).

    Google Scholar 

  • Karrer, W. &Hürlimann, H. &Cherbuliez, E. (1985). Konstitution und Vorkommen der organischen Pflanzenstoffe. Suppl. 2, Pt. 2. — 2328 pp.; Basel (Birkhäuser Verlag).

    Google Scholar 

  • Kashiwada, Y. &Nonaka, G. I. &Nishioka, I. &Chang, J. J. &Lee, K. H. (1992): Antitumor agents. 129. Tannins and related compounds as selective cytotoxic agents. — J. nat. Prod.,55: 103–143.

    Article  Google Scholar 

  • Klayman, D. L. (1985): Qinghaosu (artemisinin): An antimalarial drug from China. — Science,228: 1049–1055.

    Article  PubMed  CAS  Google Scholar 

  • Künneman, T. &Gad, G. (1996): Überleben zwischen Land und Meer — Salzwiesen. — 217 pp.; Oldenburg (Isensee Verlag).

    Google Scholar 

  • Lacaille-Dubois, M. A. &Wagner, H. (1996): A review of the biological and pharmacological activities of saponins. — Phytomedicine,2: 363–386.

    CAS  Google Scholar 

  • Lellau, T. F. &Liebezeit, G. (subm): Ethnobotany and phytochemistry of salt marsh plants from the Lower Saxonian Wadden Sea, southern North Sea. — Bot. Mar.

  • Liebezeit, G. &Künnemann, T. D. &Gad, G. (1999): Biotech-nological potential of North Sea salt marsh plants—a review of traditional knowledge. — J. Biotechnol.,70: 77–84.

    Article  CAS  Google Scholar 

  • Nakashima, H. &Murakami, T. &Yamamoto, N. &Sakagami, H. &Tanuma, S. I. &Hatano, T. &Yoshida, T. &Okuda, T. (1992): Inhibition of human immunodeficiency viral replication by tannins and related compounds. — Antiviral Res.,18: 91–103.

    Article  PubMed  CAS  Google Scholar 

  • Okuda, T. &Yoshida, T. &Hatano, T. (1989): Ellagitannins as active constituents of medicinal plants. — Planta medica,55: 117–122.

    Article  PubMed  CAS  Google Scholar 

  • Okuda, T. &Yoshida, T. &Hatano, T. (1993): Classification of oligomeric hydrolysable tannins and specificity of their occurrence in plants. — Phytochemistry,32: 507–521.

    Article  CAS  Google Scholar 

  • Olson, S. G. (1996): Curing cancer through aquaculture. — Sea Technol.,37: 89–94.

    Google Scholar 

  • Pelletier, S. W. (1983): The nature and definition of an alkaloid. — In:Pelletier, S. W. [Ed.]: Alkaloids. Chemical and Biological Perspectives, Vol. 1: 1–31; New York (Wiley).

    Google Scholar 

  • Pennisi, E. (1997): Biotechnology — In industry, extremophiles begin to make their mark. — Science,276: 705–706.

    Article  PubMed  CAS  Google Scholar 

  • Radwan, S. S. (1991): Sources of C20-polyunsaturated fatty acids for biotechnological use. — Appl. Microbiol. Biotechnol.,35: 421–430.

    Article  CAS  Google Scholar 

  • Roth, L. &Daunderer, M. &Kormann, K. (1994): Giftpflanzen — Pflanzengifte. Vorkommen, Wirkung, Therapie, Allergische und phototoxische Reaktionen. — 4th Edn.: 1092 pp.; Landsberg/Lech (Ecomed).

    Google Scholar 

  • Scalbert, A. (1991): Antimicrobial properties of tannins. — Phytochemistry,30: 3875–3883.

    Article  CAS  Google Scholar 

  • Schneider, G. &Hiller, K. (1999): Arzneidrogen. — 4th Edn.: 578 pp.; Heidelberg, Berlin, Oxford (Spektrum Akademischer Verlag).

    Google Scholar 

  • Scholz, E. (1994): Pflanzliche Gerbstoffe, Pharmakologie und Toxikologie. — Dtsch. Apotheker Ztg.,134: 3167–3179.

    Google Scholar 

  • Spencer, C. M. &Cai, Y. &Martin, R. &Gaffney, S. H. &Goulding, P. N. &Magnolato, D. &Lilley, T. H. &Haslam, E. (1988): Polyphenol complexation — some thoughts and observations. — Phytochemistry,27: 2397–2409.

    Article  CAS  Google Scholar 

  • Spilkovä, J. &Hubík, J. (1988): Biologische Wirkung von Flavonoiden. — Pharmazie in unserer Zeit,17: 1–9.

    Article  PubMed  Google Scholar 

  • Yamaguchi, K. (1997): Recent advances in the microalgal bioscene in Japan, wirth special reference to utilization of biomass and metabolites: a review. — J. appl. Phycol.,8: 487–502.

    Article  Google Scholar 

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Lellau, T.F., Liebezeit, G. Alkaloids, saponins and phenolic compounds in salt marsh plants from the Lower Saxonian Wadden Sea. Senckenbergiana maritima 31, 1–9 (2001). https://doi.org/10.1007/BF03042831

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