Abstract
In vitro direct induction of multiple shoot buds ofSolanum sarrachoides Sendt. was obtained from leaf segments using Murashige and Skoog’s basal medium supplemented with 6-γ-γ-(dimethylallylamino) purine (1·5–2·5 mgl−1). Maximum shoot bud proliferation was observed in the medium containing 2 mgl−1 and better growth of plants with well defined roots, leaves, flowers and fruits in the subsequent stages of development. Histological examination at different stages of development confirmed that the multiple buds produced on excised leaf segments arise adventitiously through the formation of numerous tiny protuberances. The complete sequence of events leading to multiple bud formation based on histological studies has been presented.
Similar content being viewed by others
References
Banerjee S, Schwemmin A M and Schwemmin D J 1985In vitro propagation ofSolanum sarrachoides Sendt.;J Plant Physiol. 119 461–466
Bhatt P N, Bhatt D P and Sussex I M 1979 Organ regeneration from leaf discs ofSolanum nigrum, S. dulcamara andS. khasianum;Z. Pflanzenphysiol. 95 355–362
Coleman W K and Greyson R I 1977 Promotion of root initiation by gibberellic acid in leaf discs of tomato (Lycopersicon esculentum) culturedin vitro;New Phytol. 78 47–54
Jensen W A 1962Botanical histochemistry (San Francisco: WH Freeman and Co.)
Mann J D 1978 Production of Solasodine for the pharmaceutical industry;Adv. Agron. 30 207–245
Murashige T and Skoog F 1962 A revised medium for rapid growth and bioassay with tobacco tissue cultures;Physiol. Plant. 15 473–497
Ross M K and Thorpe A 1973 Physiological gradients and shoot initiation in tobacco callus cultures;Plant Cell Physiol. 14 473–480
Ross M K, Thorpe A and Costerton 1973 Ultrastructural aspects of shoot initiation in tobacco callus cultures;Am. J. Bot. 60 788–795
Schilling E E 1984 Foliar flavonoids of North AmericanSolanum SectSolanum;Biochem. Syst. Ecol. 12 53–56
Thorpe T A and Murashige T 1970 Some histochemical changes underlying shoot initiation in tobacco callus cultures;Can. J. Bot. 48 277–285
Westcott R J, Grout B W W and Henshaw 1979 Rapid clonal propagation ofSolanum curtilobum cv Mallku by aseptic shoot meristem culture; inThe biology and taxonomy of the Solanaceae (eds) J G Hawkes, R N Lester and A D Skelding (London: Academic Press) pp 377–381
Zenkteller M 1972In vitro formation of plants from leaves of several species of Solanaceae family;Biochem. Physiol. Pflanz. 163 509–512
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Banerjee, N., Mukhopadhyay, S. & Sharma, A.K. Ontogenesis ofin vitro shoot bud proliferation inSolanum sarrachoides Sendt.. Proc. Indian Acad. Sci. 99, 307–312 (1989). https://doi.org/10.1007/BF03053573
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF03053573