Skip to main content
SpringerLink
Log in

High frequency somatic embryogenesis and plant regeneration from immature inflorescence cultures of two Indian cultivars of sorghum (Sorghum bicolor L. Moench)

  • Published:
Proceedings / Indian Academy of Sciences

Abstract

Somatic embryogenesis and subsequent plant regeneration were obtained from immature inflorescence segments of two cultivars ofSorghum bicolor L. Moench. Callus was induced on Murashige and Skoog’s medium supplemented with 2,4-dichlorophenoxyacetic acid (2 mg/l) and zeatin (0.1 mg/l). Transfer of calli to Murashige and Skoog medium alone or with reduced level of auxins with or without cytokinins resulted in the production of somatic embryos and plantlets. The combination of a cytokinin with Triiodobenzoic acid promoted the highest number of somatic embryoids. The best response was obtained from inflorescences 10–25 mm in length. Regenerated plants were transferred to field for further evaluation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Boyes C J and Vasil I K 1984 Plant regeneration by somatic embryogenesis from cultured young inflorescences ofSorghum arundinaceum (Desv) Stampf. var.sudanense (Sudan grass);Plant Sci. Lett. 35 153–157

    Article  Google Scholar 

  • Brar D S, Rambold S, Gamborg O and Constabel F 1979 Tissue culture of corn andSorghum;Z. Pflanzenphysiol.95 377–388

    Google Scholar 

  • Brettell R I S, Wernicke W and Thomas E 1980 Embryogenesis from cultured immature inflorescences ofSorghum bicolor;Protoplasma 104 141–148

    Article  Google Scholar 

  • Chen T H, Lam L and Chen S C 1985 Somatic embryogenesis and plant regeneration from cultured, young inflorescences ofOryza sativa L. (rice);Plant Cell Tissue Organ Culture 4 51–54

    Article  Google Scholar 

  • Eapen S and Rao P S 1985 Plant regeneration from immature inflorescence callus cultures of wheat, rye and triticale;Euphytica 34 153–159

    Article  Google Scholar 

  • Mac Kinnon C, Gunderson G and Nabors M W 1986 Plant regeneration by somatic embryogenesis from callus cultures of sweet sorghum;Plant Cell Rep.5 349–351

    Article  Google Scholar 

  • Murashige T and Skoog F 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures;Physiol. Plant. 15 473–497

    Article  CAS  Google Scholar 

  • Smith R H and Bhaskaran S 1986 Sorghum (Sorghum bicolor) (L). Moench); inBiotechnology in agriculture and forestry (ed.) Y P S Bajaj (Berlin: Springer Verlag) Vol 2, pp 220–233

    Google Scholar 

  • Thomas E, King P J and Potrykus I 1977 Shoot and embryo-like structure formation from cultured tissues ofSorghum bicolor;Naturwissen-schaften 64 587

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Biotechnology Section, Bhabha Atomic Research Centre, Trombay, 400 085, Bombay, India

    Leela George, Susan Eapen & P S Rao

Authors
  1. Leela George
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susan Eapen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P S Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

George, L., Eapen, S. & Rao, P.S. High frequency somatic embryogenesis and plant regeneration from immature inflorescence cultures of two Indian cultivars of sorghum (Sorghum bicolor L. Moench). Proc. Indian Acad. Sci. 99, 405–410 (1989). https://doi.org/10.1007/BF03053607

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03053607

Keywords

Search

Navigation