Skip to main content
SpringerLink
Log in

Diversity of lipase-producing yeasts from marine environments and oil hydrolysis by their crude enzymes

  • Ecological and Environmental Microbiology
  • Original Articles
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

Total 427 yeast strains from seawater, sediments, mud of salterns, guts of the marine fish and marine algae were obtained. After lipase activity of the yeast cultures was estimated, we found that nine yeast strains obtained in this study grown in the medium with olive oil could produce lipase. The results of routine identification and molecular methods show that they belonged toCandida intermedia YA01a,Pichia guilliermondii N12c,Candida parapsilosis 3eA2,Lodderomyces elongisporus YF12c,Candida quercitrusa JHSb,Candia rugosa wl8,Yarrowia lipolytica N9a,Rhodotorula mucilaginosa L10-2 andAureobasidium pullulans HN2.3, respectively. The optimal pHs and temperatures of lipases produced by them were between 6.0 and 8.5 and between 35 and 40°C, respectively. Majority of lipases from the yeast strains were cell-bound and only lipase fromA. pullulans HN2.3 was extracellular. Some lipases from the yeast strains could actively hydrolyse different oils, indicating that they may have potential applications in industry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bradford M.M. (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–253.

    Article  CAS  PubMed  Google Scholar 

  • Chi Z.M., Liu J., Ji J.R., Meng Z. (2001). Trehalose accumulation from starch bySaccharomycopsis fibuligera sdu. Enzyme. Microb. Technol., 28: 240–245.

    Article  CAS  PubMed  Google Scholar 

  • Chi Z.M., Liu Z., Gao L., Gong F., Ma C., Wang X., Li H. (2006). Marine yeasts and their applications in mariculture. J. Ocean Univ. China 5: 251–256.

    Article  CAS  Google Scholar 

  • Chi Z., Ma C., Wang P., Li H. (2007). Optimization of medium and cultivation conditions for alkaline protease production by the marine yeastAureobasidium pullulans. Biores. Technol., 98: 534–538.

    Article  CAS  Google Scholar 

  • Felsenstein J. (1995). PHYLIP (Phylogenetic Inference Package), Version 3.75. Distributed by author, Department of Genetics, University of Washinton, Seattle, WA.

    Google Scholar 

  • Fu X.Y., Xue C.H., Miao B.C., Li Z.T., Gao X., Yang W.G. (2005). Characterization of protease from the digestive tract of sea cucumber (Stichopus japonicus): high alkaline protease activity. Aquaculture, 246: 321–329.

    Article  CAS  Google Scholar 

  • Hasan F., Shah A.A., Hameed A. (2006). Industrial applications of microbial lipases. Enzyme. Microb. Technol., 39: 235–251.

    Article  CAS  Google Scholar 

  • Kimura M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies on nucleotide sequences. J. Mol. E., 2: 87–90.

    Article  Google Scholar 

  • Kurtzman C.P., Fell J.W. (2000). The Yeasts. A Taxonomic Study, 4th revised and enlarged edn., Elsevier Amsterdam, Lausanne, New York, Oxford, Shannon, Singapore, Tokyo.

    Google Scholar 

  • Lotti M., Monticelli S., Montesinos J.L., Brocca S., Valero F., Lafuente J. (1998). Physiological control on the expression and secretion ofCandida rugosa lipase. Chem. Phys. Lipids, 93: 143–148.

    Article  CAS  PubMed  Google Scholar 

  • Sharma R., Chistib Y., Banerjee U.C. (2001). Production, purification, characterization and applications of lipases. Biotechnol. Adv., 19: 627–662.

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J., Fritsch E.F., Maniatis T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. (Chinese translating edn.), Cold Spring Harbor Laboratory Press, Beijing, pp. 367–370.

    Google Scholar 

  • Sugita T., Takashima M., Kodama M., Tsuboi, R., Nishikawa A. (2003). Description of a new yeast species,Malassezia japonica, and its detection in patients with atopic dermatitis and healthy subjects. J. Clin. Microbiol., 40: 4695–4699.

    Article  Google Scholar 

  • Vakhlu J., Kour A. (2006). Yeast lipases: enzyme purification, biochemical properties and gene cloning. E. J. Biotechnol., 9: 1–17.

    Article  Google Scholar 

  • Wu X.Y., Jaaskelainen S., Linko Y. (1996). An investigation of crude lipase for hydrolysis, esterization and transesterification

Download references

Author information

Authors and Affiliations

  1. Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China

    Lin Wang, Zhenming Chi, Xianghong Wang, Zhiqiang Liu & Jing Li

Authors
  1. Lin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhenming Chi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xianghong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhiqiang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhenming Chi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, L., Chi, Z., Wang, X. et al. Diversity of lipase-producing yeasts from marine environments and oil hydrolysis by their crude enzymes. Ann. Microbiol. 57, 495–501 (2007). https://doi.org/10.1007/BF03175345

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03175345

Key words

Search

Navigation