Skip to main content
Log in

A high γ-aminobutyric acid-producingLactobacillus brevis isolated from Chinese traditionalpaocai

  • Food Microbiology
  • Original Articles
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

To find a high γ-aminobutyric acid-producing lactic acid bacterium, more than 1000 strains of lactic acid bacteria isolated frompaocai samples in various areas of China were screened by the ability in production of γ-aminobutyric acid, analysed with paper chromatography, HPLC and HPLC-MS. Among them, one strain NCL912 exhibited high ability to convert sodium glutamate to γ-aminobutyric acid. The strain accumulated 149.05 mM of γ-aminobutyric acid in a modified MRS medium containing 3% sodium glutamate after 48 h of static cultivation at 30 °C. This strain was identified asLactobacillus brevis according to its phenotypic and phylogenetic characteristics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adeghate E., Ponery A.S. (2002). GABA in the endocrine pancreas: cellular localization and function in normal and diabetic rats. Tissue Cell, 34: 1–6.

    Article  CAS  PubMed  Google Scholar 

  • Hagiwara H., Seki T., Ariga T. (2004). The effect of pre-germinated brown rice intake on blood glucose and PAI-1 levels in streptozotocin-induced diabetic rats. Biosci. Biotechnol. Biochem., 68: 444–447.

    Article  CAS  PubMed  Google Scholar 

  • Hao R., Schmit J.C. (1993). Cloning of the gene for glutamate decarboxylase and its expression during conidiation inNeurospora crassa. Biochem. J., 293: 735–738.

    CAS  PubMed  Google Scholar 

  • Huang J., Mei L., Sheng Q. Yao S., Lin D. (2007a). Purification and characterization of glutamate decarboxylase oflactobacillus brevis CGMCC 1306 isolated from fresh milk. Chinese J. Chem. Eng., 15: 157–161.

    Article  CAS  Google Scholar 

  • Huang J., Mei L., Xia J. (2007b). Application of artificial neural network coupling particle swarm optimization algorithm to biocatalytic production of GABA. Biotechnol. Bioeng., 96: 924–931.

    Article  CAS  PubMed  Google Scholar 

  • Jakobs C., Jaeken J., Gibson K.M. (1993). Inherited disorders of GABA metabolism. J. Inherit. Metab. Dis., 16: 704–715.

    Article  CAS  PubMed  Google Scholar 

  • Komatsuzaki N., Shima J., Kawamoto S., Momose H., Kimura T. (2005). Production of γ-aminobutyric acid (GABA) byLactobacillus paracasei isolated from traditional fermented foods. Food Microbiol., 22: 497–504.

    Article  CAS  Google Scholar 

  • Kono I., Himeno K. (2000). Changes in α-aminobutyric acid content during beni-koji making. Biosci. Biotechnol. Biochem., 64: 617–619.

    Article  CAS  PubMed  Google Scholar 

  • Manyam B.V., Katz L., Hare T.A., Kaniefski K., Tremblay R.D. (1981). Isoniazid-induced elevation of cerebrospinal fluid (CSF) GABA levels and effects on Chorea in Huntington’s disease. Ann. Neurol., 10: 35–37.

    Article  CAS  PubMed  Google Scholar 

  • Maras B., Sweeney G., Barra D., Bossa F., John R.A. (1992). The amino acid sequence of glutamate decarboxylase fromEscherichia coli. Eur. J. Biochem., 204: 93–98.

    Article  CAS  PubMed  Google Scholar 

  • Nomura M., Kimoto H., Someya Y., Furukawa S., Suzuki I. (1998). Production of γ-aminobutyric acid by cheese starters during cheese ripening. J. Dairy Sci., 81: 1486–1491.

    CAS  PubMed  Google Scholar 

  • Nomura M., Nakajima I., Fujita Y., Kobayashi M., Kimoto H., Suzuki I., Aso H. (1999).Lactococcus lactis contains only one glutamate decarboxylase gene. Microbiology, 145: 1375–1380.

    Article  CAS  PubMed  Google Scholar 

  • Nomura M., Kobayashi M., Ohmomo S., Okamoto T. (2000). Inactivation of the glutamate decarboxylase gene inLactococcus lactis subsp. cremoris. Appl. Environ. Microbiol., 66: 2235–2237.

    Article  CAS  PubMed  Google Scholar 

  • Park K.B., Oh S.H. (2007). Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Biores. Technol., 98: 1675–1679.

    Article  CAS  Google Scholar 

  • Sethi M.L. (1999). Enzyme inhibition XI: glutamate decarboxylase activity relationship with the reaction products as determined by the colorimetric and radioisotopic methods. J. Pharm. Biomed. Anal., 19: 847–54.

    Article  CAS  PubMed  Google Scholar 

  • Siragusa S., De Angelis M., Di Cagno R., Rizzello C.G., Coda R., Gobbetti M. (2007). Synthesis of gamma-aminobutyric acid by lactic acid bacteria isolated from a variety of Italian cheeses. Appl. Environ. Microbiol., 73: 7283–7290.

    Article  CAS  PubMed  Google Scholar 

  • Smith D.K., Kassam T., Singh B., Elliott J.F. (1992).Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci. J. Bacteriol., 174: 5820–5826.

    CAS  PubMed  Google Scholar 

  • Stackebrandt E., Goebel B.M. (1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol., 44: 846–849.

    Article  CAS  Google Scholar 

  • Tanasupawat S., Shida O., Okada S., Komagata K. (2000).Lactobacillus acidipiscis sp. nov. andWeissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int. J. Syst. Evol. Microbiol., 50: 1479–1485.

    CAS  PubMed  Google Scholar 

  • Ueno Y., Hayakawa K., Takahashi S., Oda K. (1997). Purification and characterization of glutamate decarboxylase fromLactobacillus brevis IFO 12005. Biosci. Biotech. Biochem., 61: 1168–1171.

    Article  CAS  Google Scholar 

  • Van der Meulen R., Scheirlinck I., Van Schoor A., Huys G., Vancanneyt M., Vandamme P., De Vuyst L. (2007). Population dynamics and metabolite target analysis of lactic acid bacteria during laboratory fermentations of wheat and spelt sourdoughs. Appl. Environ. Microbiol., 73: 4741–4750.

    Article  PubMed  CAS  Google Scholar 

  • Wong C.G., Bottiglieri T., Snead O.C. 3rd (2003). GABA, γ-hydroxybutyric acid, and neurological disease. Ann. Neurol., 6: 3–12.

    Article  CAS  Google Scholar 

  • Yokoyama S., Hiramatsu J.I., Hayakawa K. (2002). Production of γ-Aminobutyric acid from alcohol distillery lees byLactobacihs brevis IFO-12005. J. Biosci. Bioeng. 93: 95–97.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yusheng Cao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, H., Gao, D., Cao, Y. et al. A high γ-aminobutyric acid-producingLactobacillus brevis isolated from Chinese traditionalpaocai . Ann. Microbiol. 58, 649–653 (2008). https://doi.org/10.1007/BF03175570

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03175570

Key words

Navigation