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Aspects of culture:Acanthamoeba astronyxis (Ray and Hayes 1954) from Bay of Bengal coasts, India

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Proceedings: Animal Sciences

Abstract

Acanthamoeba astronyxis strains, isolated from intertidal belts of Sagar Island (21°30′ to 21°50′ N and 88°04′ to 88°08′ E) facing Bay of Bengal, were cultured in saline distilled water agar medium supplemented withEscherichia coli as food bacterium. Population growth in bay water agar, cerophyl bay water agar and soil extract distilled water agar were compared with special reference to devised saline distilled water agar medium. The overall growth showed little variation over ranges of media salinity (5–30‰). However, greater values were encountered in media of intermediate range. Isolate from mangrove litter-soil of highest high tide belt (strains) grew more efficiently in cerophyl bay water agar and soil extract distilled water agar media while mid-littoral and lowest low tide isolates preferred bay water agar and saline distilled water agar media more.

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References

  • Adams M H 1959Bacteriophages; (New York: Interscience)

    Google Scholar 

  • Bamforth S S 1981 Protist Biogeography;J. Protozool. 28 2–9

    CAS  PubMed  Google Scholar 

  • Bhattacharya A, Ghosh M and Choudhury A 1985 Seasonal population dynamics ofAcanthamoeba rhysodes (Singh 1952) in a mangrove litter-soil ecosystem of lower deltaic Bengal, India;Abstr. VII Int. Cong. Protozool., Nairobi, Kenya

  • Bovee E C and Sawyer T K 1979 Marine Flora and Fauna of the Northeastern United States. Protozoa: Sarcodina: Amoebae;NOAA Technical Report, NMFS Circular 419

  • Chang S L 1958 Cultural, cytological and ecological observations on the amoeba stage ofNaegleria gruberi;J. Gen. Microbiol. 18 565–578

    CAS  PubMed  Google Scholar 

  • Choudhury A and Ghosh M 1985 Benthic gymnamoebae as micropredator components in a non-reclaimed mangrove swamp of Gangetic-estuary, India;Abstr. VII Int. Cong. Protozool., Nairobi, Kenya

  • Davis P G, Caron D A and Sieburth J Mc N 1978 Oceanic amoebae from the North Atlantic. Culture, Distribution and Taxonomy;Trans. Am. Microsc. Soc. 97 73–88

    Article  Google Scholar 

  • Dixon A 1937 Soil protozoa; their growth on various media;Ann. Appl. Biol. 24 442–456

    Article  Google Scholar 

  • Fulton C 1970 Amoebo-flagellates as research partners: the laboratory biology ofNaegleria andTetramitus; inMethods Cell Physiol. (ed) D M Prescott (New York: Academic Press)4 341–476

    Google Scholar 

  • Ghosh M and Choudhury A 1985aCulturing gymnamoebae from saline environments of Hooghly estuary, India; Abstr. II Natl. Seminar on Mar. Intertidal Ecol., Waltair

  • Ghosh M and Choudhury A 1985bAcanthamoeba rhysodes (Singh, 1952) (Protozoa: Gymnamoebia) from mangrove environment as model organism in systems ecology: I. Population growth kinetics in relation to media salinity;Abstr. I Natl. Symp. on Biol. Utiliz. and Conser. of Mangroves, Kolhapur

  • Ghosh M and Choudhury A 1985cAcanthamoeba rhysodes (Singh 1952) (Protozoa: Gymnamoebia) from mangrove environment as model organism in systems ecology: II. ‘Food’ as it modulates the population growth kinetics;Abstr. I Natl. Symp. on Biol. Utiliz. and Conser. of Mangroves Kolhapur

  • Ghosh M and Choudhury A 1986 Growth ofAcanthamoeba culbertsoni (Singh and Das 1970) andA. rhysodes (Singh 1952) (Protozoa: Gymnamoebia) under simplified co-cultural condition;Proc. Indian Acad. Sci. (Anim. Sci.) 95 469–473

    Article  Google Scholar 

  • Ghosh M, Bhattacharya A and Choudhury A `985 Competition amongst intertidal benthicAcanthamoeba spp. of Hooghly estuary, India;Abstr. VII Int. Cong. Protozool., Nairobi, Kenya

  • Menapace D, Klein D A, McClellan J F and Mayeux J V 1975 A simplified overlay plaque technique for evaluating responses of small free living amoebae in grassland soil;J. Protozool. 22 405–410

    Google Scholar 

  • Page F C 1966Cryptodifflugia operculata n. sp. (Rhizopodea: Arcellinida, Cryptodifflugiidae) and the status of the genusCryptodifflugia;Trans. Am. Microsc. Soc. 85 506–515

    Article  Google Scholar 

  • Page F C 1967 Taxonomic critieria for limax amoebae, with descriptions of 3 new species ofHartmannella and 3 ofVahlkampfia;J. Protozool. 14 499–521

    CAS  PubMed  Google Scholar 

  • Page F C 1970 Two new species ofParamoeba from Maine;J. Protozool. 17 421–427

    Google Scholar 

  • Page F C 1980 A key to marine species ofVanella (Sarcodina: Gymnamoebia), with descriptions of new species;J. Mar. Biol. Assoc. UK 60 929–946

    Article  Google Scholar 

  • Page F C 1981 A light and electron microscopical study ofProtacanthamoeba caledonica n. sp., Type-species ofProtacanthamoeba n. g. (Amoebida, Acanthamoebidae);J. Protozool. 28 70–78

    Google Scholar 

  • Prescott D M 1956 Mass and clone culturing ofAmoeba proteus andChaos chaos;C. R. Trav. Lab. Carlsberg Ser. Chim. 30 1–12

    CAS  Google Scholar 

  • Prescott D M and James T W 1955 Culturing ofAmoeba proteus onTetrahymena;Exp. Cell Res. 8 256–258

    Article  CAS  PubMed  Google Scholar 

  • Ray D L and Hayes R E 1954hartmannella astronyxis: A new species of free-living amoeba;J. Morphol. 95 159–188

    Article  Google Scholar 

  • Sawyer T K 1971 Cultivation and identification of free living marine amoebae from Chesapeake Bay, Maryland;Trans. Am. Microsc. Soc. 90 43–51

    Article  Google Scholar 

  • Sawyer T K 1975 Marine amoebae from surface waters of Chincoteague Bay, Virginia. Two new genera and nine new species within the family Mayorellidae, Flabellulidae and Stereomyxidae;Trans. Am. Microsc. Soc. 94 71–92

    Article  Google Scholar 

  • Sawyer T K 1980 Marine amoebae from clean and stressed bottom sediments of the Atlantic Ocean and Gulf of Mexico;J. Protozool. 27 13–32

    Google Scholar 

  • Sieburth J Mc N 1979Sea Microbes (New York: Oxford University Press)

    Google Scholar 

  • Sieburth J Mc N, Willis P J, Johnson K M, Burney C M, Lavoie D M, Hinga K R, Caron D A, French F W III, Johnson P W and Davis P G 1976 Dissolved organic matter and heterotrophic microneuston in the surface microlayers of the North Atlantic;Science 194 1415–1418

    Article  CAS  PubMed  Google Scholar 

  • Singh B N 1945 The selection of bacterial food by soil amoebae, and the toxic effects of bacterial pigments and other products on soil protozoa;Br. J. Exp. Pathol. 26 316–325

    Google Scholar 

  • Singh B N 1955 culturing soil protozoa and estimating their numbers in soil; inSoil Zoology (ed) D K McE Kevan (London: Butterworths) pp 403–411

    Google Scholar 

  • Singh B N 1973 Current status of the problem of exogenous and endogenous amoebiasis;J. Sci. Ind. Res. 32 399–432

    Google Scholar 

  • Singh B N 1975Pathogenic and Nonpathogenic Amoebae. (New York: Wiley)

    Google Scholar 

  • Singh B N and Hanumaiah V 1979Studies on pathogenic and nonpathogenic amoebae and the bearing of nuclear division and locomotive form and behaviour on the classification of the order Amoebida (Monogr. No. 1 of the Assn. of Microbiol. of India; Published by Indian J. Microbiol.) pp 1–80

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Authors and Affiliations

  1. Ecology Division, Zoological Survey of India, 8, Lindsay Street, 700 087, Calcutta, India

    Mrinal Ghosh

  2. SD Marine Biological Research Institute, Sagar Island, West Bengal, India

    Mrinal Ghosh & Amalesh Choudhury

  3. Department of Marine Science, University of Calcutta, 35 Ballygunge Circular Road, 700 019, Calcutta, India

    Mrinal Ghosh & Amalesh Choudhury

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  1. Mrinal Ghosh
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  2. Amalesh Choudhury
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Ghosh, M., Choudhury, A. Aspects of culture:Acanthamoeba astronyxis (Ray and Hayes 1954) from Bay of Bengal coasts, India. Proc. Indian Acad. Sci. (Anim. Sci.) 96, 63–69 (1987). https://doi.org/10.1007/BF03179393

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  • DOI: https://doi.org/10.1007/BF03179393

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