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Detection and quantification of patulin and griseofulvin by high pressure liquid chromatography in different strains of Penicillium griseofulvum Dierckx

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Abstract

Patulin and griseofulvin production by twelve strains ofPenicillium griseofulvum Dierckx, eleven of which were isolated from pistachio (Pistacia vera) nuts and the other was supplied by the Spanish Collection of Type Culture, was investigated. Six strains of the eleven isolated had ability to produce patulin and griseofulvin in Yes medium. All the strains studied had no ability to produce patulin in Wickerham medium. Griseofulvin production was significant in both media but higher in Wickerham.

These metabolites were separated and determined in the chloroform extracts of cultures by high performance liquid chromatography with ultraviolet detection. The best conditions were: acetonitrile — water (45∶55) as mobile phase, a flow rate of 2.0 mL/min and a μ Bondapack C18 column.

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Authors and Affiliations

  1. Department of Biotechnology, Polytechnic University of Valencia, Camino de Vera, E-46022, Valencia, (Spain)

    M Jiménez & E Hernández

  2. Department of Microbiology, ETSIA, Polytechnic University of Catalunya, Lleida, (Spain)

    V Sanchis

  3. Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Dr Moliner 50, Burjassot, Valencia, (Spain)

    R Mateo

Authors
  1. M Jiménez
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  2. V Sanchis
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  3. R Mateo
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  4. E Hernández
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Jiménez, M., Sanchis, V., Mateo, R. et al. Detection and quantification of patulin and griseofulvin by high pressure liquid chromatography in different strains of Penicillium griseofulvum Dierckx. Mycotox Res 4, 59–66 (1988). https://doi.org/10.1007/BF03192100

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  • DOI: https://doi.org/10.1007/BF03192100

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