Skip to main content
SpringerLink
Log in

Morphogenesis in Long-term Maintained Immature Embryo-derived Callus of Wheat (Triticum aestivum L) - Histological Evidence for Both Somatic Embryogenesis and Organogenesis

  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Callus induced from immature embryos of wheat cv Kharchia 65 on Murashige and Skoog’s medium containing 2.5 mg l1 2,4-D was maintained In the regenerable state by subculturing every 5-6 weeks on medium supplemented with 2,4-D (2.5 mg l-1) and proline (10 mg l-1). Addition of proline helped maintain morphogenic competence for over four years. The regenerating callus was analysed histologically about one year after first induction. Both somatic embryogenesis and shoot organogenesis were seen in the same callus tissue that contained typical stages of somatic embryoid development and evidence for the de novo shoot bud formation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Shimada T, Japanese J Genet, 53 (1978) 371.

    Article  Google Scholar 

  2. Sears RG & Deckard EL, Crop Science, 22 (1982) 546.

    Article  Google Scholar 

  3. Ozlas-Akins P & Vasil IK, Protoplasma, 110 (1982) 95.

    Article  Google Scholar 

  4. He DG, Tanner G & Scott KJ, Plant Sci, 45 (1986) 119.

    Article  CAS  Google Scholar 

  5. Varshney A, Kant T, Sharma VK, Rao A & Kothari SL, Cereal Res Comm, 24 (1996) 409.

    Google Scholar 

  6. Fennell S, Bohorova N, VanGinkel M, Crossa J & Hosington D, Theor Appl Genet, 92 (1996) 163.

    Article  Google Scholar 

  7. Altpeter F, Vasil V, Srivastava V, Stoger E & Vasil IK, Plant Cell Rep, 16 (1996) 12.

    Article  CAS  Google Scholar 

  8. Altpeter F, Vasil V, Srivastava V & Vasil IK, Nature Biotech, 14 (1996) 1155.

    Article  CAS  Google Scholar 

  9. Chin TC & Scott KJ, Ann Bot, 41 (1977) 473.

    CAS  Google Scholar 

  10. Sharma VK, Rao A, Varshney A & Kothari SL, Plant Cell Rep, 15 (1995) 227.

    CAS  Google Scholar 

  11. Wernicke W & Milkovits L, Protoplasma, 131 (1986) 131.

    Article  CAS  Google Scholar 

  12. Ahuja PS, Pental D & Cocking EC, Z Pflanzenzuecht, 89 (1982) 139.

    Google Scholar 

  13. Rajyalakshmi K, Grover A, Maheshwari N, Tyagi AK & Maheshwari SC, Physiol Plant, 82 (1991) 617.

    Article  CAS  Google Scholar 

  14. Varshney A, Kant T & Kothari SL, Biol Plant, 40 (1998) 137.

    Article  Google Scholar 

  15. Maheshwari N, Rajyalakshml K, Baweja K, Dhir SK, Chowdhry CN & Maheshwari SC, Critical Rev Plant Sci, 14 (1995) 149.

    Google Scholar 

  16. Ozias-Akins P & Vasil IK, Protoplasma, 117 (1983) 40.

    Article  Google Scholar 

  17. Magnusson E & Bornmann CH, Physiol Plant, 63 (1985) 137.

    Article  Google Scholar 

  18. He DG, Yang YM & Scott KJ, Plant Sci, 57 (1988) 225.

    Article  Google Scholar 

  19. Lorz H, Gobel E & Brown P, Plant Breeding, 100 (1988) 1.

    Article  Google Scholar 

  20. Kothari SL & Chandra N, J Ind Bot Soc, 74A (1995) 323.

    Google Scholar 

  21. Kothari SL, Poddar K & Chandra N, In Plant tissue culture and molecular biology (PS Srivastava, Editor). Narosa, New Delhi (1998) pp 380–401.

    Google Scholar 

  22. He DG, Yang YM, Bertram J & Scott KJ, Plant Sci, 68 (1990) 103.

    Article  Google Scholar 

  23. Chen WH, Davey MR, Power, JB & Cocking EC, J Exp Bot, 39 (1988) 251.

    Article  CAS  Google Scholar 

  24. Oka S, Saito N & Kawaguchi H, Ann Bot, 76 (1995) 487.

    Article  Google Scholar 

  25. Kachhwaha S, Varshney A, Kothari SL, Cereal Res Comm, 25 (1997) 117.

    Google Scholar 

  26. Lowe K, Taylor DB, Ryan PK & Peterson E, Plant Sci, 41 (1985) 125.

    Article  Google Scholar 

  27. Thomas E, King PJ & Potrykus I, Naturwissenschaften, 64 (1977) 587.

    Article  Google Scholar 

  28. Kothari SL, Sharma V & Chandra N, J Indian Bot Soc, 73 (1994) 203.

    CAS  Google Scholar 

  29. Sivadas P, Chandra N & Kothari SL, Phytomorphology, 42 (1992) 203.

    Google Scholar 

  30. Vasil IK, J Plant Physiol, 128 (1987) 183.

    Article  Google Scholar 

  31. Rajyalakshmi K, Dheer SK, Maheshwari N & Maheshwari SC, Plant Breeding, 101 (1988) 80.

    Article  Google Scholar 

  32. Redway FA, Vasil V, Lu D & Vasil IK, Theor App Genet, 79 (1990) 609.

    Article  Google Scholar 

  33. Murashige T & Skoog F, Physiol Plantarum, 15 (1962) 473.

    Article  CAS  Google Scholar 

  34. Nabors MW, Heyser JW, Dykes TA & Demott KJ, Planta, 157 (1983) 385.

    Article  Google Scholar 

  35. Suprasanna P, Rao KV & Reddy GM, Cereal Res Comm, 22 (1994) 79.

    Google Scholar 

  36. Ozawa K & Komamine A, Theor Appl Genet, 77 (1989) 205.

    Article  Google Scholar 

  37. Chowdhry CN, Tyagi AK, Maheshwari N & Maheshwari SC, Plant Cell Tiss Org Cult, 32 (1993) 357.

    Article  CAS  Google Scholar 

  38. Rengel Z & Jelaska S, Acta Bot Croat, 45 (1986) 71.

    CAS  Google Scholar 

  39. Holme IB, Krogstrup P & Hensen J, Plant Cell Tiss Org Cult, 50 (1997) 203.

    Article  CAS  Google Scholar 

  40. Bhaskaran S & Smith RH, Crop Sci, 30 (1990) 1328.

    Article  CAS  Google Scholar 

  41. Vasil V, Lu C-Y & Vasil IK, Protoplasma, 127 (1985) 1.

    Article  Google Scholar 

  42. Wakizuka T & Yamaguchi T, Ann Bot, 60 (1987) 331.

    Google Scholar 

  43. Springer WD, Green CE & Kohn KA, Protoplasma, 101 (1979) 269.

    Article  Google Scholar 

  44. Shimada T & Yamada Y, Japanese J Genet, 54 (1979) 379.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, University of Rajasthan, Jalpur, 302 004, India

    S. L. Kothar & Alok Varshney

  2. IPK, Gatersleben, Germany

    Alok Varshney

Authors
  1. S. L. Kothar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alok Varshney
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kothar, S.L., Varshney, A. Morphogenesis in Long-term Maintained Immature Embryo-derived Callus of Wheat (Triticum aestivum L) - Histological Evidence for Both Somatic Embryogenesis and Organogenesis. J. Plant Biochem. Biotechnol. 7, 93–98 (1998). https://doi.org/10.1007/BF03263042

Download citation

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03263042

Key words

Search

Navigation