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In Vitro Clonal Propogation of Coleus forskohlii via Direct Shoot Organogenesis from Selected Leaf Explants

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Abstract

Present study provides an easy and efficient protocol for large scale clonal propagation of Coleus forskohlii, a threatened medicinal plant of commercial importance. Basal leaf lamina excised from upper three nodes of shoot was used as explant and its size, position, orientation and season of collection were initially optimized to select the most responsive explant condition. Enhanced shoot production and proliferation has been achieved on medium containing 2 μM BA + 0.1 μM NAA wherein, a highest number of 35 shoots/explant were produced. The regenerated shoots of varied length (3–5 cm) were transferred to root induction medium comprising of IBA, NAA and IAA (1–5 μM) in half-strength MS medium to determine the most suitable shoot length for proper root induction. Rooted plantlets were acclimatized in field conditions after proper hardening. Histological analysis was also carried out to confirm the nature of origin of shoot buds from leaf explants.

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Abbreviations

CAMP:

cyclic adenosine monophosphate

PGR:

Plant growth regulators

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, Uttar Pradesh, India

    Aastha Sahai & Anwar Shahzad

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  1. Aastha Sahai
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  2. Anwar Shahzad
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Correspondence to Anwar Shahzad.

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Sahai, A., Shahzad, A. In Vitro Clonal Propogation of Coleus forskohlii via Direct Shoot Organogenesis from Selected Leaf Explants. J. Plant Biochem. Biotechnol. 19, 223–228 (2010). https://doi.org/10.1007/BF03263344

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  • DOI: https://doi.org/10.1007/BF03263344

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