Skip to main content
SpringerLink
Log in

In Vitro Clonal Propagation and Medium Term Conservation of Brahmi [Bacopa monnieri (L) Wettst]

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A protocol has been developed for in vitro clonal propagation leading to conservation of Bacopa monnieri (L) Wettst, a medicinal plant of high commercial potential with legendary reputation as a memory vitalizer. Single node explants when cultured on Murashige and Skoog’s medium supplemented with BA (0.2 mg l-1), showed active shoot proliferation (22.2 shoots/ explant in 8 weeks) without callus formation. Rooting was achieved on the same medium. The generated shoots could also be conserved for 12 months with high survival rate (up to 100%). The regenerants upon transfer to soil showed no morphological variation as compared with the donor plants. The medium optimized in the present study was applied for culture establishment and conservation of a total of 15 Brahmi accessions procured from different regions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

BA:

6-benzyladenine

IAA:

indole-3-acetic acid

MS:

Murashige and Skoog's medium

NAA:

α-naphthaleneacetic acid

References

  1. Tiwari V, Tiwari KN & Singh BD, Plant Cell Tiss Org Cult, 66 (2001) 9.

    Article  CAS  Google Scholar 

  2. Mohapatra HP & Rath SP, Indian J Exp Biol, 43 (2005) 373.

    PubMed  CAS  Google Scholar 

  3. Tiwari V, Tiwari KN & Singh BD, Phytomorphology, 50 (2000) 337.

    Google Scholar 

  4. Chandel KPS, Shukla G & Sharma N, Biodiversity in medicinal and aromatic plants in India, National Bureau of Plant Genetic Resources, New Delhi, India (1996) p 239.

    Google Scholar 

  5. Natesh S, In Biotechnology in horticultural and plantation crops (KL Chadha, PN Ravindran, L Sahijram, Editors) Malhotra Publ. House, New Delhi, India (2000) pp 548–561.

  6. Tejavathi DH & Shailaja KS, Phytomorphology, 49 (1999) 447.

    Google Scholar 

  7. Tiwari V, Singh, BD & Tiwari KN, Plant Cell Rep, 17 (1998) 538.

    Article  CAS  Google Scholar 

  8. Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.

    Article  CAS  Google Scholar 

  9. Gomez KA & Gomez AA, Statistical procedures for agricultural research, John Willey & Sons, New York (1984).

    Google Scholar 

  10. Sharma N & Sharma B, Cryo Lett, 24 (2003) 181.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110 012, India

    Neelam Sharma, Richa Satsangi, Ruchira Pandey & S. Vimala Devi

Authors
  1. Neelam Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richa Satsangi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ruchira Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Vimala Devi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Neelam Sharma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharma, N., Satsangi, R., Pandey, R. et al. In Vitro Clonal Propagation and Medium Term Conservation of Brahmi [Bacopa monnieri (L) Wettst]. J. Plant Biochem. Biotechnol. 16, 139–143 (2007). https://doi.org/10.1007/BF03321990

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03321990

Key words

Search

Navigation