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Somatic Embryogenesis in Asparagus densiflorus (Kunth) Jessop cv Sprengeri

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Abstract

An efficient protocol has been developed for plant regeneration in Asparagus densiflorus (Kunth) Jessop cv Sprengeri (Asparagaceae) through somatic embryogenesis from spear sections. Callus culture was initiated on Murashige and Skoog (MS) medium formulation containing α-naphthaleneacetic acid (5.37 μM), indole-3-acetic acid (5.71 μM) and 6-benzylaminopurine (2.22 μM). The callus became embryogenic by transferring to 2, 4-dichlorophenoxyacetic acid (4.52 μM) containing MS medium. Somatic embryos developed and matured vigorously on MS medium with NAA (1.07 μM), 6-γ-γ- dimethylaminopurine (9.84 μM) and abscisic acid (1.89 μM). Mature bipolar embryos were converted efficiently into plants on MS medium in the presence of low level of kinetin (2.32 μM). Regenerated plants showed 80% survival after transfer to field. These plants were all diploid (2n=60). Peroxidase activity was maximum in the embryogenic callus as documented from the gel as well as spectrophotometric analysis.

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Abbreviations

ABA:

Abscisic acid

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

2ip:

6-γ-γ-dimethylaminopurine

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog

NAA:

α-naphthaleneacetic acid

p-CPA:

pchlorophenoxyacetic acid

References

  1. Stephens CT, De Vries RM & Sink KC, Hort Sci, 24 (1989) 365.

    Google Scholar 

  2. Desjardins Y, High-tech and micropropagation III. Biotechnology in agriculture and forestry, Vol 19 (YPS Bajaj, Editor). Springer, Berlin Heidelberg New York (1992) pp 26–41.

  3. Nag C, Ph.D. Thesis, University of Calcutta (2006) 1.

    Google Scholar 

  4. Mukhopadhyay S, Overney S, Yelle S & Desjardins Y, J Plant Biochem Biotech, 11 (2002) 57.

    Article  Google Scholar 

  5. Mukhopadhyay S & Desjardins Y, J Plant Physiol, 144 (1994) 94.

    Article  Google Scholar 

  6. Benmoussa M, Mukhopadhyay S & Desjardins Y, Plant Cell Rep, 17 (1997) 123.

    Article  CAS  Google Scholar 

  7. Murashige T & Skoog F, Physiol Plant, 15 (1962) 158.

    Article  Google Scholar 

  8. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ, J Biol Chem, 193 (1951) 265.

    PubMed  CAS  Google Scholar 

  9. Klein RM & Edsall PC, Phytomorph, 18 (1968) 204.

    Google Scholar 

  10. Puchooa D, African J Biotech, 3 (2004) 576.

    CAS  Google Scholar 

  11. Krishna Raj S & Vasil IK, In In vitro embryogenesis in plants, (TA Thorpe, Editor), Kluwer Academic Publishers, Dordrecht, The Netherlands (1995) pp 471–540.

  12. Grossmann K, Trends Plant Sci, 5 (2000) 506.

    Article  PubMed  CAS  Google Scholar 

  13. Lo Schiavo F, Pitto L, Giuliano G, Torti G, Nuti Ronchi V, Marazziti D, Vergara R, Oselli S & Terzi M, Theor Appl Genet, 77 (1989) 325.

    Article  CAS  Google Scholar 

  14. Litz RE & Gray DJ, World J Microbiol Biotech, 11 (1995) 416.

    Article  Google Scholar 

  15. Zimmermann JL, Plant Cell, 5 (1993) 1411.

    Google Scholar 

  16. Sink KC, Elmer WH & Stephens CT, Acta Hort, 271 (1990) 117.

    Google Scholar 

  17. Fieldes MA & Gerhardt KE, Plant Sci, 132 (1998) 89.

    Article  CAS  Google Scholar 

  18. Carrillo-Castaneda G & Mata A, Biotechnol Aplicada, 17 (2000) 225.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, 700 019, India

    Chitralekha Nag Dasgupta & Sandip Mukhopadhyay

  2. Plant Biotechnology Group, Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata, 700 032, India

    Madhumita J. Mukhopadhyay

Authors
  1. Chitralekha Nag Dasgupta
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  2. Madhumita J. Mukhopadhyay
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  3. Sandip Mukhopadhyay
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Corresponding author

Correspondence to Sandip Mukhopadhyay.

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Dasgupta, C.N., Mukhopadhyay, M.J. & Mukhopadhyay, S. Somatic Embryogenesis in Asparagus densiflorus (Kunth) Jessop cv Sprengeri. J. Plant Biochem. Biotechnol. 16, 145–149 (2007). https://doi.org/10.1007/BF03321991

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  • DOI: https://doi.org/10.1007/BF03321991

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