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Determination of Seed Storage Proteins and Total Isoflavones in Wild and Cultivated Soybeans

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Abstract

Two soybean components namely, storage proteins and isoflavone content in a wild and three cultivated soybean genotypes were characterized and compared. The storage proteins, β-conglycinin and glycinin were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and two major storage proteins and their subunits were characterized using mass spectrometry. The three isoflavones, aglycon and the nine conjugated forms were separated by HPLC (high performance liquid chromatography) and identified by comparison of retention time, ultraviolet and mass spectral analyses. Comparison between the number of 2D-PAGE protein spots of the storage protein subunits and HPLC area of twelve isoflavones was also evaluated. The analysis of proteins and isoflavones from the wild genotype and the three cultivated genotypes suggested possible interactions between proteins and isoflavones. The same wild genotype, which showed significant statistical differences in β-conglycinin and glycinin protein profiles also revealed considerable reduction in total isoflavones (> 55%) content.

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Abbreviations

HPLC:

high performance liquid chromatography

2D-PAGE:

two-dimensional polyacrylamide gel electrophoresis

GLM:

general linear model

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Authors and Affiliations

  1. Soybean Genomics and Improvement Laboratory, Plant Sciences Institute, Beltsville, MD, 20705-3000, USA

    Savithiry S. Natarajan & Perry Cregan

  2. Food Composition and Methods Development Laboratory, BHNRC, ARS, USDA, Beltsville, MD, 20705-3000, USA

    Devanand L. Luthria

  3. Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742-4452, USA

    Qijian Song

Authors
  1. Savithiry S. Natarajan
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  2. Devanand L. Luthria
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  3. Qijian Song
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  4. Perry Cregan
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Corresponding author

Correspondence to Savithiry S. Natarajan.

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Natarajan, S.S., Luthria, D.L., Song, Q. et al. Determination of Seed Storage Proteins and Total Isoflavones in Wild and Cultivated Soybeans. J. Plant Biochem. Biotechnol. 19, 115–118 (2010). https://doi.org/10.1007/BF03323448

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  • DOI: https://doi.org/10.1007/BF03323448

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