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Isolation and application of a wild strain photosynthetic bacterium to environmental waste management

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Abstract

A new photosynthetic bacterium isolate was morphologically identified as a non-motile rod-shape gram-negative bacterium. It produced a dark red culture under phototrophic condition, reproduced by budding and formed a lamellar intracytoplasmic membrane system parallel to cytoplasmic membrane, which contained bacteriochlorophyll a and caratenoids. It’s physiological and nutrient requirement tests gave indication that the isolate thrived and multiplied in varied environmental conditions. It was consequently named Z08 and identified as Rhodobacter sphaeroides by 16SrDNA. Adaptation of Z08 to biodegradation of two environmentally concerned wastewaters, i.e. soybean and pharmaceutical wastewaters, attested its potential in wastewater bioremediation. Z08 adaptation in a suspended batch photobioreactor treating pharmaceutical wastewater at 3500lx radiation recorded best result after wastewater dilution of 1:4 with concomitant chemical oxygen demand reduction, biomass yield and specific growth of 50 %, 780 mg/L and 0.015/h, respectively at the lowest hydraulic retention time of three days. Furthermore, gas chromatography mass spectra analyses of treated and raw pharmaceutical wastewater indicated that high molecular weight recalcitrant compounds found in the pharmaceutical wastewater were transformed to less toxic and acceptable lower molecular weight substances through biodegradation. Whilst Z08 treatment of soybean wastewater under natural light intensity radiation recorded 80 % reduction, 1540 mg/L and 0.025/h for chemical oxygen demand, biomass and specific growth rate respectively regardless of the food to microorganism ratio. This preliminary investigation showed that isolate Z08 has some toxic tolerance level which could detoxify refractory substances with great potential for cell protein recovery in high organic strength wastewater. Therefore, strain Z08 could be employed in biodegradation of contaminated wastewater streams.

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Authors and Affiliations

  1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China

    E. I. Madukasi M.Sc., Ph.D. (Research scientist, Research student), H. Chunhua B.Sc. (Senior student) & G. Zhang Ph.D. (Lecturer)

  2. Federal Institute of Industrial Research, Oshodi, Lagos, Nigeria

    E. I. Madukasi M.Sc., Ph.D. (Research scientist, Research student)

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  1. E. I. Madukasi M.Sc., Ph.D.
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  2. H. Chunhua B.Sc.
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  3. G. Zhang Ph.D.
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Correspondence to G. Zhang Ph.D..

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Madukasi, E.I., Chunhua, H. & Zhang, G. Isolation and application of a wild strain photosynthetic bacterium to environmental waste management. Int. J. Environ. Sci. Technol. 8, 513–522 (2011). https://doi.org/10.1007/BF03326237

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  • DOI: https://doi.org/10.1007/BF03326237

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