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Environmental car exhaust pollution damages human sperm chromatin and DNA

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Abstract

Objective: The adverse role of traffic pollutants on male fertility is well known. Aim of this study was to evaluate their effects on sperm chromatin/DNA integrity. Methods: To accomplish this, 36 men working at motorway tollgates and 32 unexposed healthy men (controls) were enrolled. All of them were interviewed about their lifestyle. Hormone, semen samples, and environmental and biological markers of pollution were evaluated. Sperm chromatin and DNA integrity were evaluated by flow cytometry following propidium iodide staining and TUNEL assay, respectively. Results: LH, FSH, and testosterone serum levels were within the normal range in tollgate workers. Sperm concentration, total sperm count, total and progressive motility, and normal forms were significantly lower in these men compared with controls. Motorway tollgate workers had a significantly higher percentage of spermatozoa with damaged chromatin and DNA fragmentation, a late sign of apoptosis, compared with controls. A significant direct correlation was found between spermatozoa with damaged chromatin or fragmented DNA and the length of occupational exposure, suggesting a time-dependent relationship. Conclusion: This study showed that car exhaust exposure has a genotoxic effect on human spermatozoa. This may be of relevant importance not only for the reproductive performance of the men exposed, but also for the offspring health.

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Authors and Affiliations

  1. Sezione di Endocrinologia, Andrologia e Medicina Interna, Dipartimento di Medicina Interna e Patologie Sistemiche, Università di Catania, Policlinico “G. Rodolico”, Via S. Sofia 78, Bldg 4, Room 2C19, 95123, Catania, Italy

    A. E. Calogero, S. La Vignera, R. A. Condorelli, A. Perdichizzi, D. Valenti, P. Asero, R. D’Agata, L. O. Vicari & E. Vicari

  2. Departments of Preventive Medical Sciences, Italy

    U. Carbone & B. Boggia

  3. Molecular and Clinical Endocrinology and Oncology, University of Naples “Federico II”, Naples, Italy

    N. De Rosa, G. Lombardi & M. De Rosa

Authors
  1. A. E. Calogero
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  2. S. La Vignera
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  3. R. A. Condorelli
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  4. A. Perdichizzi
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  5. D. Valenti
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  6. P. Asero
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  7. U. Carbone
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  8. B. Boggia
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  9. N. De Rosa
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  10. G. Lombardi
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  11. R. D’Agata
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  12. L. O. Vicari
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  13. E. Vicari
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  14. M. De Rosa
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Correspondence to A. E. Calogero.

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Calogero, A.E., La Vignera, S., Condorelli, R.A. et al. Environmental car exhaust pollution damages human sperm chromatin and DNA. J Endocrinol Invest 34, e139–e143 (2011). https://doi.org/10.1007/BF03346722

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  • DOI: https://doi.org/10.1007/BF03346722

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