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The effect of GHRH on TSH release in rats in vivo and in vitro

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Abstract

In man, GHRH has been shown to potentiate the TSH-releasing activity of TRH. To study the way by which GHRH affects TRH-stimulated TSH release, we examined the effect of GHRH (1–29)NH2 on basal and stimulated TSH secretion in intact male rats and superfused dispersed rat pituitary cells. In the intact rats, GHRH(1–29)NH2 potentiated TRH-stimulated TSH release in the evening, but potentiation was not observed in the morning and in dispersed pituitary cells. Basal TSH levels were not changed by GHRH(1–29)NH2. It is concluded that GHRH(1–29)NH2 potentiates the TSH-releasing activity of TRH in the evening in rats possibly through suprahypophyseal disinhibition.

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Authors and Affiliations

  1. Department of Endocrinology, University Hospital, Leiden, The Netherlands

    B. J. Looij Jr., A. C. Nieuwenhuijzen Kruseman, F. Roelfsema, D. van der Heide, R. P. L. M. Hoogma & J. P. Schröder van der Elst

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  1. B. J. Looij Jr.
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  2. A. C. Nieuwenhuijzen Kruseman
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  3. F. Roelfsema
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  4. D. van der Heide
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  5. R. P. L. M. Hoogma
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  6. J. P. Schröder van der Elst
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Looij, B.J., Kruseman, A.C.N., Roelfsema, F. et al. The effect of GHRH on TSH release in rats in vivo and in vitro . J Endocrinol Invest 18, 214–219 (1995). https://doi.org/10.1007/BF03347804

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  • DOI: https://doi.org/10.1007/BF03347804

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