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A new solid-phase immunoradiometric assay for anti-thyroglobulin autoantibody

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Abstract

A newly developed sensitive and quantitative immunoradiometric assay (IRMA) for anti-thyroglobulin (anti-Tg) autoantibody is described. Serum samples to be tested are added to wells of polyvinyl microtiter plates coated with human thyroglobulin. After removal of the unbound material, anti-Tg antibody is determined by adding purified 125l-anti-human immunoglobulin G antibody. Using 1.0 μl of serum anti-Tg antibody was detected in 81.2% of patients with Hashimoto’s thyroidits or idiopathic myxedema (n = 32), in 46.4% of those with Graves’ disease (n = 28), in 11.9% of subjects with other thyroid disorders (n = 42) and in 4.2% of normal controls (n = 71). Similar percentages of positive tests were observed by passive hemagglutination (PH) and a good correlation was found between the antibody levels determined by the two techniques. Using larger amounts of serum (100 μl) detectable anti-Tg antibody by IRMA was found in the majority of patients with thyroid autoimmune disorders who had negative PH tests. Quantitative measurements of anti-Tg antibody by IRMA could be obtained by using purified anti-Tg antibody as standard reference. The minimum detectable amount of anti-Tg antibody was 0.5 ng. The present method is proposed as a simple and convenient technique for quantitative measurement of any antibody, using wells coated with the appropriate antigen.

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Authors and Affiliations

  1. Cattedra di Patologia Medica 2, Université di Pisa, c/o Ente Ospedaliero di Pisa, via Roma 67, 56100, Pisa, Italy

    Stefano Mariotti, S. Pisani & A. Russova

  2. Cattedra di Endocrinologia e Medicina Costituzionale, Université di Pisa, Via Roma 67, 56100, Pisa, Italy

    A. Pinchera

Authors
  1. Stefano Mariotti
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  2. S. Pisani
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  3. A. Russova
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  4. A. Pinchera
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Mariotti, S., Pisani, S., Russova, A. et al. A new solid-phase immunoradiometric assay for anti-thyroglobulin autoantibody. J Endocrinol Invest 5, 227–233 (1982). https://doi.org/10.1007/BF03348328

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  • DOI: https://doi.org/10.1007/BF03348328

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