Abstract
Rhizoctonia solani is the most important pathogen involved in cotton seedling disease in Egypt. Variations in the antagonistic activity of 20 isolates belonging to Trichoderma harzianum and T. longi-brachiatum were evaluated in vitro, against three isolates of Rhizoctonia solani in dual culture. There was highly significant interaction between isolates of Trichoderma and R. solani. All isolates of Trichoderrma spp. showed varying levels of antagonism against R. solani. Similarly, the interaction between Trichoderma isolates and R. solani was a highly significant source of variation in surviving seedlings under greenhouse conditions. In vitro and in vivo antagonism were not correlated, which indicated that, in vitro inhibition of the pathogen was not predictive of in vivo biocontrol by an individual antagonist. Microsatellite-primed PCR analysis was used to evaluate the genetic variation among Trichoderma isolates. The analysis divided the isolates into two main groups. Grouping the isolates based on cluster analysis of their DNA profiles matched that based on their morphological taxonomy. However, no congruency was found between cluster analyses obtained by PCR and cluster analysis by efficiency parameters of the biocontrol agents.
Zusammenfassung
Rhizoctonia solani ist das bedeutendste, an einer Baumwollkeimlingskrankheit in Ägypten beteiligte pilzliche Pathogen. Unterschiede in der antagonistischen Aktivität von 20 Isolaten von Trichoderma harzianum und T. longibrachiatum wurden in vitro gegenüber drei Isolaten von R. solani in gemeinsamer Kultur untersucht. Die Interaktionen zwischen Trichoderma-Isolaten und R. solani waren hoch signifikant. Die Ausprägung des Antagonismus der Trichoderma-Isolate gegenüber R. solani variierte. In ähnlicher Weise erwies sich die Wechselwirkung zwischen den Trichoderma-Isolaten und R. solani als hoch signifikanter Variationsfaktor bei überlebenden Keimlingen im Gewächshaus. Die antagonistischen Wirkungen in vitro und in vivo waren nicht miteinander korreliert. Eine antagonistische Wirkung gegenüber dem Pathogen in vitro erlaubte keine Voraussage des Bekämpfungspotenzials eines einzelnen Trichoderma-Isolats in vivo. Die genetische Variation zwischen den Trichoderma-Isolaten wurde mittels einer Mikrosatelliten-PCR bestimmt, wobei die Isolate zwei Hauptgruppen zugeordnet werden konnten. Die Zuordnung der Isolate anhand einer Cluster-Analyse ihrer DNA-Profile deckte sich mit ihrer morphologischen Einordnung, während keine Übereinstimmung zwischen den Cluster-Analysen der DNA-Profile und des antagonistischen Potenzials bestand.
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Asran-Amal, A., Abd-Elsalam, K.A., Omar, M.R. et al. Antagonistic potential of Trichoderma spp. against Rhizoctonia solani and use of M13 microsatellite-primed PCR to evaluate the antagonist genetic variation. J Plant Dis Prot 112, 550–561 (2005). https://doi.org/10.1007/BF03356152
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DOI: https://doi.org/10.1007/BF03356152