Abstract
Recruitment in social insects often involves not only inducing nestmates to leave the nest, but also communicating crucial information about finding profitable food sources. Although bumblebees transmit chemosensory information (floral scent), the transmission mechanism is unknown as mouth-to-mouth fluid transfer (as in honeybees) does not occur. Because recruiting bumblebees release a pheromone in the nest that triggers foraging in previously inactive workers, we tested whether this pheromone helps workers learn currently rewarding floral odours, as found in food social learning in rats. We exposed colonies to artificial recruitment pheromone, paired with anise scent. The pheromone did not facilitate learning of floral scent. However, we found that releasing floral scent in the air of the colony was sufficient to trigger learning and that learning performance was improved when the chemosensory cue was provided in the nectar in honeypots; probably because it guarantees a tighter link between scent and reward, and possibly because gustatory cues are involved in addition to olfaction. Scent learning was maximal when anise-scented nectar was brought into the nest by demonstrator foragers, suggesting that previously unidentified cues provided by successful foragers play an important role in nestmates learning new floral odours.
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References
Arenas A, Fernández VM, Farina WM (2007) Floral odor learning within the hive affects honeybees’ foraging decisions. Naturwissenschaften 94:218–222
Clark CW, Mangel M (1986) The evolutionary advantages of group foraging. Theor Popul Biol 40:45–75
De Brito Sanchez G, Ortigão-Farias JR, Gauthier M, Liu F, Giurfa M (2007) Taste perception in honeybees: just a taste of honey? Arthropod–Plant Interactions 1:69–76
Dornhaus A, Chittka L (1999) Evolutionary origins of bee dances. Nature 401:38
Dornhaus A, Chittka L (2001) Food alert in bumblebees (Bombus terrestris): possible mechanisms and evolutionary implications. Behav Ecol Sociobiol 50:570–576
Dornhaus A, Chittka L (2004) Information flow and regulation of foraging activity in bumble bees (Bombus spp.). Apidologie 35:183–192
Dornhaus A, Brockman A, Chittka L (2003) Bumble bees alert to food with pheromone from tergal gland. J Comp Physiol A 189:47–51
Dornhaus A, Klügl F, Oechslein C, Puppe F, Chittka L (2006) Benefits of recruitment in honey bees: effects of ecology and colony size in an individual-based model. Behav Ecol 17:336–344
Farina WM, Grüter C, Díaz PC (2005) Social learning of floral odours inside the honeybee hive. Proc R Soc B 272:1923–1928
Galef BG, Wigmore SW (1983) Transfer of information concerning distant foods: a laboratory investigation of the ‘information-centre’ hypothesis. Anim Behav 31:748–758
Galef BG, Mason JR, Preti G, Bean NJ (1988) Carbon disulfide: a semiochemical mediating socially-induced diet choice in rats. Physiol Behav 42:119–124
Giurfa M, Núñez JA (1992) Honeybees mark with scent and reject recently visited flowers. Oecologia 89:113–117
Grüter C, Acosta LE, Farina WM (2006) Propagation of olfactory information within the honeybee hive. Behav Ecol Sociobiol 60:707–715
Heltne PG, Wojcik JF, Pook AG (1981) Goeldi’s monkey, genus Callimico. In: Coimbra-Filho AF, Mittermeier RA (eds) Ecology and behavior of neotropical primates. vol. 1. Academia Brasileira de Ciencias, Rio de Janeiro
Hölldobler B, Wilson EO (1970) Recruitment trails in the harvester ant Pogonomyrmex badius. Psyche 77:385–399
Jakobsen HB, Kristjansson K, Rohde B, Terkildsen M, Olsen CE (1995) Can social bees be influenced to choose a specific feeding station by adding the scent of the station to the hive air? J Chem Ecol 21:1635–1648
Judd TM, Sherman PW (1996) Naked mole-rats recruit colony mates to food sources. Anim Behav 52:957–969
Leadbeater E, Chittka L (2007) Social learning in insects—from miniature brains to consensus building. Curr Biol 17:R703–R713
Mena Granero A, Guerra Sanz JM, Egea González FJ, Martínez Vidal JL, Dornhaus A, Ghani J, Roldan Serrano A, Chittka L (2005) Chemical compounds of the foraging recruitment pheromone in bumblebees. Naturwissenschaften 92:371–374
Molet M, Chittka L, Stelzer RJ, Streit S, Raine NE (2008) Colony nutritional status modulates worker responses to foraging recruitment pheromone in the bumblebee Bombus terrestris. Behav Ecol Sociobiol 62:1919–1926. doi:10.1007/s00265-008-0623-3
Pham-Delègue MH, Roger B, Charles R, Masson C (1990) Effet d’une pré-exposition olfactive sur un comportement d’orientation en olfactomètre dynamique a quatre voies chez l’abeille (Apis mellifera L.). Insect Soc 37:181–187
Raine NE, Ings TC, Ramos-Rodriguez O, Chittka L (2006) Intercolony variation in learning performance of a wild British bumblebee population (Hymenoptera: Apidae: Bombus terrestris audax). Entomol Gen 28:241–256
Robertson HM, Warr CG, Carlson JR (2003) Molecular evolution of the insect chemoreceptor gene superfamily in Drosophila melanogaster. Proc Natl Acad Sci U S A 100:14537–14542
Saleh N, Scott AG, Bryning GP, Chittka L (2007) Distinguishing signals and cues: bumblebees use general footprints to generate adaptive behaviour at flowers and nest. Arthropod–Plant Interactions 1:119–127
Sandoz JC, Laloi D, Odoux JF, Pham-Delègue MH (2000) Olfactory information transfer in the honeybee: compared efficiency of classical conditioning and early exposure. Anim Behav 59:1025–1034
Vergoz V, Schreurs HA, Mercer AR (2007) Queen pheromone blocks aversive learning in young worker bees. Science 317:384–386
von Frisch K (1923) Über die “Sprache” der Bienen—Eine tierpsychologische Untersuchung. Zool Jahrb 40:1–186
von Frisch K (1967) The dance language and orientation of bees. Harvard University Press, Cambridge
Wenner AM, Wells PH, Johnson DL (1969) Honey bee recruitment to food sources: olfaction or language? Science 164:84–86
Acknowledgments
We thank Syngenta Bioline Bees for supplying the bumblebee colonies, Hélène Müller, Oscar Ramos Rodríguez and Daniel Stollewerk for the help with experiments and colony maintenance and four anonymous referees for useful comments. This work was supported by a grant from the Natural Environment Research Council (NE/F523342/1).
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Molet, M., Chittka, L. & Raine, N.E. How floral odours are learned inside the bumblebee (Bombus terrestris) nest. Naturwissenschaften 96, 213–219 (2009). https://doi.org/10.1007/s00114-008-0465-x
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DOI: https://doi.org/10.1007/s00114-008-0465-x