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Medicago truncatula EST-SSRs reveal cross-species genetic markers for Medicago spp.

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Abstract

Expressed sequence tags (ESTs) are important resources for gene discovery and molecular marker development. From over 147,000 ESTs of Medicago truncatula, we have identified 4,384 ESTs containing perfect simple sequence repeats (EST-SSR) of di-, tri-, tetra- or pentanucleotides. Six hundred sixteen primer pairs (PPs) were designed and screened over a panel of eight genotypes representing six Medicago spp. and subspecies. Nearly, 74% (455) of the PPs produced characteristic SSR bands of expected size length in at least one Medicago species. Four hundred six (89%) of these 455 PPs produced SSR bands in all eight genotypes tested. Only 17 PPs were M. truncatula -specific. High levels of polymorphism (>70%) were detected for these markers in alfalfa, M. truncatula, and other annual medics. About 48% of the reported markers are part of gene transcripts linked to putative functions. Our results indicate that the SSR markers developed from M. truncatula ESTs are valuable genetic markers for the Medicago genus. These markers will be useful in establishing the genomic relationships of M. truncatula to important forage legume crops such as alfalfa and other annual medics.

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Acknowledgements

We thank Drs. Douglas Cook and Ian Ray for the plant materials of their respective mapping populations used in this study. This research work was funded by The Samuel Roberts Noble Foundation.

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  1. I. Eujayl

    Present address: Department of Food Science & Human Nutrition, Washington State University, E202 Food Quality Building, WWQL, Pullman, WA 99164, USA

Authors and Affiliations

  1. The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA

    I. Eujayl, M. K. Sledge, L. Wang, G. D. May, K. Chekhovskiy, J. C. Zwonitzer & M. A. R. Mian

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  1. I. Eujayl
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  2. M. K. Sledge
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  3. L. Wang
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Correspondence to M. A. R. Mian.

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Communicated by H.C. Becker

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Eujayl, I., Sledge, M.K., Wang, L. et al. Medicago truncatula EST-SSRs reveal cross-species genetic markers for Medicago spp.. Theor Appl Genet 108, 414–422 (2004). https://doi.org/10.1007/s00122-003-1450-6

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  • DOI: https://doi.org/10.1007/s00122-003-1450-6

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