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The genetic map of finger millet, Eleusine coracana

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Abstract

Restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), expressed-sequenced tag (EST), and simple sequence repeat (SSR) markers were used to generate a genetic map of the tetraploid finger millet (Eleusine coracana subsp. coracana) genome (2n = 4x = 36). Because levels of variation in finger millet are low, the map was generated in an inter-subspecific F2 population from a cross between E. coracana subsp. coracana cv. Okhale-1 and its wild progenitor E. coracana subsp. africana acc. MD-20. Duplicated loci were used to identify homoeologous groups. Assignment of linkage groups to the A and B genome was done by comparing the hybridization patterns of probes in Okhale-1, MD-20, and Eleusine indica acc. MD-36. E. indica is the A genome donor to E. coracana. The maps span 721 cM on the A genome and 787 cM on the B genome and cover all 18 finger millet chromosomes, at least partially. To facilitate the use of marker-assisted selection in finger millet, a first set of 82 SSR markers was developed. The SSRs were identified in small-insert genomic libraries generated using methylation-sensitive restriction enzymes. Thirty-one of the SSRs were mapped. Application of the maps and markers in hybridization-based breeding programs will expedite the improvement of finger millet.

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Acknowledgments

MMD and S gratefully acknowledge PhD studentships from the Sir Halley Stewart Fund (UK) and Commonwealth Scholarship Commission (UK), respectively. Part of the work was funded by a grant from the McKnight Foundation and a training grant from the Kirkhouse Trust (UK) to KMD.

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Authors and Affiliations

  1. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

    Mathews M. Dida,  Srinivasachary, Mike D. Gale & Katrien M. Devos

  2. Department of Crop and Soil Sciences and Department of Plant Biology, University of Georgia, Athens, GA, 30602, USA

    Mathews M. Dida,  Srinivasachary & Katrien M. Devos

  3. Department of Botany and Horticulture, Maseno University, P.O. Box 333, Maseno, Kenya

    Mathews M. Dida

  4. Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA

    Sujatha Ramakrishnan & Jeffrey L. Bennetzen

  5. Department of Genetics, University of Georgia, Athens, GA, 30602, USA

    Jeffrey L. Bennetzen

Authors
  1. Mathews M. Dida
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  2. Srinivasachary
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  3. Sujatha Ramakrishnan
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  4. Jeffrey L. Bennetzen
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  5. Mike D. Gale
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  6. Katrien M. Devos
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Correspondence to Katrien M. Devos.

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Communicated by E. Guiderdoni.

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Dida, M.M., Srinivasachary, Ramakrishnan, S. et al. The genetic map of finger millet, Eleusine coracana . Theor Appl Genet 114, 321–332 (2007). https://doi.org/10.1007/s00122-006-0435-7

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  • DOI: https://doi.org/10.1007/s00122-006-0435-7

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