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Mapping genes Lr53 and Yr35 on the short arm of chromosome 6B of common wheat with microsatellite markers and studies of their association with Lr36

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Abstract

The rust resistance genes Lr53 and Yr35, transferred to common wheat from Triticum dicoccoides, were reported previously to be completely linked on chromosome 6B. Four F 3 families were produced from a cross between a line carrying Lr53 and Yr35 (98M71) and the leaf rust and stripe rust susceptible genotype Avocet “S” and were rust tested using Puccinina triticina pathotype 53-1,(6),(7),10,11 and Puccinia striiformis f. sp. tritici pathotype 110 E143 A+. The homozygous resistant lines produced infection types of “;1−” and “;N” to these pathotypes, respectively. The Chi-squared tests indicated goodness-of-fit of the data for one leaf rust gene and one stripe rust gene segregation. Linkage analysis using this population demonstrated recombination of 3% between the genes. Microsatellite markers located on the short arm of chromosome 6B were used to map the genes, with the markers cfd1 and gwm508 being mapped approximately 1.1 and 4.5 cM, respectively, proximal to Lr53. Additional studies of the relationship between Lr36, also located on the short arm of chromosome 6B, and Lr53 indicated that the two genes were independent.

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Acknowledgments

The first author would like to thank Professor R.A. McIntosh for providing seeds of Manitou and Neepawa and also express thanks to the Iranian government for a scholarship to undertake PhD studies at The University of Sydney. The authors also extend thanks to the Australian Grains Research and Development Corporation for financial support, and Professor G.F. Marais for provision of the Yr35-Lr53 stock 98M71 used in this study.

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  1. N. A. Dadkhodaie

    Present address: Department of Agronomy and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Fars, 71441-65186, Iran

Authors and Affiliations

  1. Plant Breeding Institute, The University of Sydney, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia

    N. A. Dadkhodaie, H. Karaoglou, C. R. Wellings & R. F. Park

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  1. N. A. Dadkhodaie
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  2. H. Karaoglou
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  3. C. R. Wellings
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  4. R. F. Park
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Correspondence to R. F. Park.

Additional information

Communicated by M. Sorrells.

C. R. Wellings is seconded from Industry and Investment NSW.

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Dadkhodaie, N.A., Karaoglou, H., Wellings, C.R. et al. Mapping genes Lr53 and Yr35 on the short arm of chromosome 6B of common wheat with microsatellite markers and studies of their association with Lr36. Theor Appl Genet 122, 479–487 (2011). https://doi.org/10.1007/s00122-010-1462-y

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  • DOI: https://doi.org/10.1007/s00122-010-1462-y

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