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Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat worldwide. It is essential to identify new genes for effective resistance against the disease. Durum wheat PI 480148, originally from Ethiopia, was resistant in all seedling tests with several predominant Pst races in the US under controlled greenhouse conditions and at multiple locations subject to natural infection for several years. To map the resistance gene(s) and to transfer it to common wheat, a cross was made between PI 480148 and susceptible common wheat genotype Avocet S (AvS). Resistant F3 plants with 42 chromosomes were selected cytologically and by testing with Pst race PST-100. A total of 157 F4 plants from a single F3 plant with 2n = 42 tested with PST-100 segregated in a 3 resistant: 1 susceptible ratio, indicating that a single dominant gene from PI 480148 conferred resistance. Using the F3:4 population and the resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) markers, the gene was mapped to the long arm of chromosome 2B. SSR marker Xwmc441 and RGAP marker XLRRrev/NLRRrev 350 flanked the resistance gene by 5.6 and 2.7 cM, respectively. The effective resistance of the gene to an Australian Pst isolate virulent to Yr5, which is also located on 2BL and confers resistance to all US Pst races, together with an allelism test of the two genes, indicated that the gene from PI 480148 is different from Yr5 and should be a new and useful gene for resistance to stripe rust. Resistant common wheat lines with plant types similar to AvS were selected for use in breeding programs.

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References

  • Abdalla O, Dieseth JA, Singh RP (1992) Breeding durum wheat at CIMMYT. In: Rajaram S, Saari EE, Hettel GP (eds) Durum wheats: challenge and opportunities. CIMMYT Wheat Special Report No. 9, CIMMYT, Mexico, pp 1–13

  • Blanco IA, Rajaram S, Krostad WE (2001) Agronomic potential of synthetic hexaploid wheat-derived populations. Crop Sci 41:670–676

    Article  Google Scholar 

  • Blanco A, Gadaleta A, Cenci A, Carluccio AV, Abdelbacki AM, Simeone R (2008) Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theor Appl Genet 117:135–142

    Article  PubMed  CAS  Google Scholar 

  • Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337

    Article  Google Scholar 

  • Chen XM (2012) High-temperature adult-plant resistance, key for sustainable control of stripe rust. Am. J. Plant Sci. Biotechnol (in press)

  • Chen XM, Line RF (1992a) Identification of stripe rust resistance genes in wheat genotypes used to differentiate North American races of Puccinia striiformis. Phytopathology 82:1428–1434

    Article  Google Scholar 

  • Chen XM, Line RF (1992b) Inheritance of stripe rust resistance in wheat cultivars used to differentiate races of Puccinia striiformis in North America. Phytopathology 82:633–637

    Article  Google Scholar 

  • Chen XM, Line RF, Jones SS (1995) Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compair, Fielder, Lee, and Lemhi and interactions of aneuploid wheats with races of Puccinia striiformis. Phytopathology 85:375–381

    Article  Google Scholar 

  • Chen XM, Line RF, Leung H (1998) Genome scanning for resistance gene analogs in rice, barley, and wheat by high-resolution electrophoresis. Theor Appl Genet 97:345–355

    Article  CAS  Google Scholar 

  • Chen XM, Moore M, Milus EA, Long DL, Line RF, Marshall D, Jackson L (2002) Wheat stripe rust epidemics and races of Puccinia striiformis f. sp. tritici in the United States in 2000. Plant Dis 86:39–46

    Article  Google Scholar 

  • Chen XM, Soria MA, Yan GP, Sun J, Dubcovsky J (2003) Development of sequence tagged site and cleaved amplified polymorphic sequence markers for wheat stripe rust resistance gene Yr5. Crop Sci 43:2058–2064

    Article  CAS  Google Scholar 

  • Chen XM, Penman L, Wan AM, Cheng P (2010) Virulence races of Puccinia striiformis f. sp. tritici in 2006 and 2007 and development of wheat stripe rust and distributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States. Can J Plant Pathol 32:315–333

    Article  Google Scholar 

  • Cheng P (2008) Molecular mapping of a gene for resistance to stripe rust in spring wheat cultivar IDO377s and identification of a new race of Puccinia striiformis f. sp. tritici virulent on IDO377s. M.S. Thesis, Washington State University pp 87

  • Cheng P, Chen XM (2010) Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theor Appl Genet 121:195–204

    Article  PubMed  CAS  Google Scholar 

  • Chhuneja P, Kaur S, Garg T, Ghai M, Prashar M, Bains NS, Goel RK, Keller B, Dhaliwal HS, Singh K (2008) Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat. Theor Appl Genet 116:313–324

    Article  PubMed  CAS  Google Scholar 

  • Feldman M, Millet E (1993) Methodologies for identification, allocation and transfer of quantitative genes from wild emmer into cultivated wheat. In: Li ZS, Xin ZY (eds) Proceedings of the 8th international wheat genetics symposium. China Agricultural Scientech Press, Beijing, pp 19–27

  • Flor HH (1971) Current status of the gene-for-gene concept. Annu Rev Phytopathol 9:275–296

    Article  Google Scholar 

  • Hu G, Hulbert SH (1996) Construction of ‘compound’ rust resistance genes in maize. Euphytica 87:45–51

    Article  CAS  Google Scholar 

  • Huang XR, Cöster H, Ganal MW, Röder MS (2003) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theor Appl Genet 106:1379–1389

    PubMed  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg I (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Li Q, Chen XM, Wang MN, Jing JX (2010) Yr45, a new wheat gene for stripe rust resistance mapped on the long arm of chromosome 3D. Theor Appl Genet 122:189–197

    Article  PubMed  Google Scholar 

  • Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America. 1968–87 USDA-ARS, Technical bulletin no. 1788

  • Ma H, Singh RP, Mujeeb-Kazi A (1995) Resistance to stripe rust in Triticum turgidum, T. tauschii and their synthetic hexaploids. Euphytica 82:117–124

    Article  Google Scholar 

  • Ma H, Singh RP, Abdalla O (1997a) Resistance to stripe rust in five durum wheat cultivars. Plant Dis 81:27–30

    Article  Google Scholar 

  • Ma H, Singh RP, Mujeeb KA (1997b) Resistance to stripe rust in durum wheats, A-genome diploids, and their amphiploids. Euphytica 94:279–286

    Article  Google Scholar 

  • Ma JX, Zhou RH, Dong YS, Wang LF, Wang XM, Jia JZ (2001) Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica 120:219–226

    Article  CAS  Google Scholar 

  • Macer RCF (1966) The formal and monosomic genetic analysis of stripe rust (Puccinia striiformis) resistance in wheat. In: Proceedings of the 2nd International Wheat Genetics Symposium, Lund, Sweden 1963; Hereditas Suppl 2:127–142

  • Mamluk OF (1992) Durum wheat diseases in West Asia and North Africa (WANA). In: Rajaram S, Saari EE, Hettel GP (eds) Durum wheats: challenges and opportunities. CIMMYT Wheat Special Report No. 9, CIMMYT, Mexico, pp 89–106

  • McIntosh RA, Lagudah ES (2000) Cytogenetic studies in wheat XVIII. Gene Yr24 for resistance to stripe rust. Plant Breed 119:81–83

    Article  CAS  Google Scholar 

  • McIntosh RA, Hart GE, Devos KM, Gale MD, Rogers WJ (1998) Catalogue of gene symbols for wheat. In: Proceedings of the 9th International Wheat Genetics Symposium, vol 5, pp 139–142

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2009) Catalogue of gene symbols: 2009 supplement. In: KOMUGI-Integrated Wheat Science Database. http://www.grs.nig.ac.jp/wheat/komugi (accessed November 28, 2011)

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2010) Catalogue of gene symbols: 2010 supplement. In: KOMUGI-Integrated Wheat Science Database. http://www.grs.nig.ac.jp/wheat/komugi (accessed November 28, 2011)

  • McIntosh R, Dubcovsky J, Rogers J, Morris C, Appels R, Xia X (2011) Catalogue of gene symbols for wheat: 2011 supplement. Annu Wheat Newsl 56:273–282

    Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations (random amplified polymorphic DNA/restriction fragment length polymorphism). Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Murphy LR, Santra D, Kidwell K, Yan GP, Chen XM, Campbell KG (2009) Linkage maps of wheat stripe rust resistance genes Yr5 and Yr15 for use in marker assisted selection. Crop Sci 49:1786–1790

    Article  CAS  Google Scholar 

  • Nagarajan S, Nayar SK, Bahadur P (1986) Race 13 (67 S8) virulent on Triticum spelta var. album in India. Plant Dis 70:173

    Article  Google Scholar 

  • Ren RS, Wang MN, Chen XM, Zhang ZJ (2012) Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527. Theor Appl Genet 125:847–857

    Article  PubMed  CAS  Google Scholar 

  • Riede CR, Anderson JA (1996) Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci 36:905–909

    Article  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Schulte E, Wittekind D (1989) Standardization of the Feulgen-Schiff technique. Histochem Cell Biol 91:321–331

    Article  CAS  Google Scholar 

  • Sharma-Poudyal D, Chen XM (2011) Models for predicting potential yield loss of wheat caused by stripe rust in the US Pacific Northwest. Phytopathology 101:544–554

    Article  PubMed  CAS  Google Scholar 

  • Shi ZX, Chen XM, Line RF, Leung H, Wellings CR (2001) Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome 44:509–516

    PubMed  CAS  Google Scholar 

  • Smith PH, Hadfield J, Hart NJ, Koebner RM, Boyd LA (2007) STS markers for the wheat yellow rust resistance gene Yr5 suggest a NBS-LRR-type resistance gene cluster. Genome 50:259–265

    Article  PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Sui XX, Wang MN, Chen XM (2009) Molecular mapping of a stripe rust resistance gene in spring wheat cultivar Zak. Phytopathology 99:1209–1215

    Article  PubMed  CAS  Google Scholar 

  • Uauy C, Brevis JC, Chen XM, Khan I, Jackson L, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J (2005) High-temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105

    Article  PubMed  CAS  Google Scholar 

  • Wan AM, Chen XM (2012) Virulence, frequency, and distribution of races of Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei identified in the United States in 2008 and 2009. Plant Dis 96:67–74

    Article  Google Scholar 

  • Wellings CR, McIntosh RA (1990) Puccinia striiformis f. sp. tritici in Australasia: pathogenic changes during the first 10 years. Plant Pathol 39:316–325

    Article  Google Scholar 

  • Yan GP, Chen XM, Line RF, Wellings CR (2003) Resistance gene-analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theor Appl Genet 106:636–643

    PubMed  CAS  Google Scholar 

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Acknowledgments

This research was supported by the US Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-014-00D), Vogel Foundation (Project No. 13C-3061-3824) and Washington Wheat Commission (Project No. 13C-3061-3925). PPNS No. 0603, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Project Number WNP00663, Washington State University, Pullman, WA 99164-6430, USA. A scholarship from the China Scholarship Council to Liangsheng Xu is gratefully acknowledged. The research was also a part of the Northwest A&F University 111 Project supported by the Ministry of Education of China (Project No. B07049). We are grateful to Dr. Colin Wellings for providing the Australian stripe rust isolate. We thank Drs. Robert McIntosh and Michael Pumphrey for critical reviews of the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    L. S. Xu & Z. S. Kang

  2. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    L. S. Xu, M. N. Wang, P. Cheng, S. H. Hulbert & X. M. Chen

  3. Wheat Genetics, Quality, Physiology and Disease Research Unit, US Department of Agriculture-Agricultural Research Service (USDA-ARS), Pullman, WA, 99164-6430, USA

    X. M. Chen

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  1. L. S. Xu
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  3. P. Cheng
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  4. Z. S. Kang
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Correspondence to X. M. Chen.

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Communicated by P. Hayes.

L. S. Xu and M. N. Wang made equal contributions.

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Xu, L.S., Wang, M.N., Cheng, P. et al. Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat. Theor Appl Genet 126, 523–533 (2013). https://doi.org/10.1007/s00122-012-1998-0

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