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Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato

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Abstract

Late blight, caused by the oomycete pathogen Phytophthora infestans (Mont.) de Bary, is a devastating disease for tomato and potato crops. In the past decades, many late blight resistance (R) genes have been characterized in potato. In contrast, less work has been conducted on tomato. The Ph-3 gene from Solanum pimpinellifolium was introgressed into cultivated tomatoes and conferred broad-spectrum resistance to P. infestans. It was previously assigned to the long arm of chromosome 9. In this study, a high-resolution genetic map covering the Ph-3 locus was constructed using an F2 population of a cross between Solanum lycopersicum CLN2037B (containing Ph-3) and S. lycopersicum LA4084. Ph-3 was mapped in a 0.5 cM interval between two markers, Indel_3 and P55. Eight putative genes were found in the corresponding 74 kb region of the tomato Heinz1706 reference genome. Four of these genes are resistance gene analogs (RGAs) with a typical nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4 domain. Each RGA showed high homology to the late blight R gene Rpi-vnt1.1 from Solanum venturii. Transient gene silencing indicated that a member of this RGA family is required for Ph-3-mediated resistance to late blight in tomato. Furthermore, this RGA family was also found in the potato genome, but the number of the RGAs was higher than in tomato.

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Acknowledgments

We thank Dr. Jack Vossen for his efforts in revising the manuscript. This study was funded by the National High Technology Research and Development Program of China (863 Program, No. 2012AA100101); the National Natural Science Foundation of China (31000909 and 31171975), the Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS) (201011) and the Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture of the People’s Republic of China.

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Authors and Affiliations

  1. The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancunnandajie 12, Beijing, 100081, People’s Republic of China

    Chunzhi Zhang, Lei Liu, Zheng Zheng, Yuyan Sun, Longxi Zhou, Yuhong Yang, Feng Cheng, Zhonghua Zhang, Xiaowu Wang, Sanwen Huang, Bingyan Xie, Yongchen Du, Yuling Bai & Junming Li

  2. Wageningen UR Plant Breeding, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Yuling Bai

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  1. Chunzhi Zhang
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  2. Lei Liu
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  3. Zheng Zheng
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  4. Yuyan Sun
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  5. Longxi Zhou
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  13. Yuling Bai
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Correspondence to Junming Li.

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Communicated by C. Gebhardt.

C. Zhang and L. Liu equally contributed to this work.

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Zhang, C., Liu, L., Zheng, Z. et al. Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato. Theor Appl Genet 126, 2643–2653 (2013). https://doi.org/10.1007/s00122-013-2162-1

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