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Fine mapping and candidate gene analysis of the dominant glandless gene Gl e 2 in cotton (Gossypium spp.)

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Dominant glandless gene Gl e 2 was fine-mapped to a 15 kb region containing one candidate gene encoding an MYC transcription factor, sequence and expression level of the gene were analyzed.

Abstract

Cottonseed product is an excellent source of oil and protein. However, this nutrition source is greatly limited in utilization by the toxic gossypol in pigment glands. It is reported that the Gl e 2 gene could effectively inhibit the formation of the pigment glands. Here, three F2 populations were constructed using two pairs of near isogenic lines (NILs), which differ nearly only by the gland trait, for fine mapping of Gl e 2 . DNA markers were identified from recently developed cotton genome sequence. The Gl e 2 gene was located within a 15-kb genomic interval between two markers CS2 and CS4 on chromosome 12. Only one gene was identified in the genomic interval as the candidate for Gl e 2 which encodes a family member of MYC transcription factor with 475-amino acids. Unexpectedly, the results of expression analysis indicated that the MYC gene expresses in glanded lines while almost does not express in glandless lines. These results suggest that the MYC gene probably serves as a vital positive regulator in the organogenesis pathway of pigment gland, and low expression of this gene will not launch the downstream pathway of pigment gland formation. This is the first pigment gland-related gene identification in cotton and will facilitate the research on glandless trait, cotton MYC proteins and low-gossypol cotton breeding.

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Acknowledgments

The work was supported by grants from the National Hi-Tech R&D Program of China (No. 2013AA102601) and the National Natural Science Foundation of China (No. 31271768).

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Authors and Affiliations

  1. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China

    Hailiang Cheng, Cairui Lu, Changsong Zou, Youping Zhang, Qiaolian Wang, Juan Huang, Xiaoxu Feng, Pengfei Jiang, Wencui Yang & Guoli Song

  2. USDA-ARS, Southern Plains Agricultural Research Center, Crop Germplasm Research Unit, 2881 F&B Road, College Station, TX, 77845, USA

    John Z. Yu

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  1. Hailiang Cheng
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  2. Cairui Lu
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Correspondence to Guoli Song.

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The experiments comply with the current laws of the countries in which they were performed.

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Communicated by M. Xu.

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Cheng, H., Lu, C., Yu, J.Z. et al. Fine mapping and candidate gene analysis of the dominant glandless gene Gl e 2 in cotton (Gossypium spp.). Theor Appl Genet 129, 1347–1355 (2016). https://doi.org/10.1007/s00122-016-2707-1

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