Abstract
Offspring of somatic hybrids between the zero-erucic acid rapeseed cv Hanna and Lesquerella fendleri were analysed regarding their fatty acid profiles. In the first back-cross generation one plant was found that produced a seed containing up to 16.5% erucic acid and 15% eicosaenoic acid (Line 1), as well as a seed having 4.3% ricinoleic acid (Line 2). This was interpreted as due to a contribution of elongase and hydroxylase genes from the L. fendleri genome since these two fatty acids are not produced in the recipient rapeseed cultivar Hanna. Crosses between Line 1 and cv Hanna resulted in the production of seeds with 35% erucic acid (F2). Furthermore, crosses between the F2 plants and the rapeseed cultivar Gulle, producing 35% erucic acid in the seeds, resulted in F3 seeds with 48% erucic acid. The highest amount of erucic acid, 61.5%, was found in the F6 generation after crossing Line 1 with a high erucic acid rapeseed line, HEAR, followed by self-fertilisation for two generations. When performing Southern-blot analysis on the F6 plants, seven of the nine analysed plants hybridised with the L. fendleri species-specific repetitive probe. The presence of the hydroxylase gene was also observed in the F6 generation of Line 1 according to Southern-blot analysis. Hybridisation with a hydroxylase probe was seen although no hydroxy fatty acids could be detected in any of the F6 plants. In parallel, Line 2 was crossed with HEAR cv Gulle and self fertilised. No hydroxy fatty acids were detected in the F2 generation of Line 2 and no specific hybridisation patterns could be found in the Southern-blot analysis.
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Received: 12 December 1998 / Accepted: 4 January 1999
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Schröder-Pontoppidan, M., Skarzhinskaya, M., Dixelius, C. et al. Very long chain and hydroxylated fatty acids in offspring of somatic hybrids between Brassica napus and Lesquerella fendleri. Theor Appl Genet 99, 108–114 (1999). https://doi.org/10.1007/s001220051214
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DOI: https://doi.org/10.1007/s001220051214