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Saccharomyces cerevisiae strain UFMG 905 protects against bacterial translocation, preserves gut barrier integrity and stimulates the immune system in a murine intestinal obstruction model

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Abstract

Probiotic is a preparation containing microorganisms that confers beneficial effect to the host. This work assessed whether oral treatment with viable or heat-killed yeast Saccharomyces cerevisiae strain UFMG 905 prevents bacterial translocation (BT), intestinal barrier integrity, and stimulates the immunity, in a murine intestinal obstruction (IO) model. Four groups of mice were used: mice undergoing only laparotomy (CTL), undergoing intestinal obstruction (IO) and undergoing intestinal obstruction after previous treatment with viable or heat-killed yeast. BT, determined as uptake of 99mTc-E. coli in blood, mesenteric lymph nodes, liver, spleen and lungs, was significantly higher in IO group than in CTL group. Treatments with both yeasts reduced BT in blood and all organs investigated. The treatment with both yeasts also reduced intestinal permeability as determined by blood uptake of 99mTc-DTPA. Immunological data demonstrated that both treatments were able to significantly increase IL-10 levels, but only viable yeast had the same effect on sIgA levels. Intestinal lesions were more severe in IO group when compared to CTL and yeasts groups. Concluding, both viable and heat-killed cells of yeast prevent BT, probably by immunomodulation and by maintaining gut barrier integrity. Only the stimulation of IgA production seems to depend on the yeast viability.

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Acknowledgments

The study was supported by grants from Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Conselho Nacional de Pesquisa (CNPq). The authors thank Dr. Carlos A. Rosa for providing the yeast S. cerevisiae 905.

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Authors and Affiliations

  1. Departamento De Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, C. P. 486, 30161-970, Belo Horizonte, MG, Brazil

    Simone V. Generoso, Mirelle Viana, Rosana Santos & Valbert N. Cardoso

  2. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Flaviano S. Martins

  3. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Flaviano S. Martins & Jacques R. Nicoli

  4. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Rosa M. E. Arantes

  5. Hospital Santa Casa, Núcleo de Pesquisa em Pós-Graduação, Belo Horizonte, MG, Brazil

    José A. N. Machado

  6. Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Maria I. T. D. Correia

Authors
  1. Simone V. Generoso
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  2. Mirelle Viana
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  3. Rosana Santos
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  4. Flaviano S. Martins
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  5. José A. N. Machado
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  6. Rosa M. E. Arantes
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  7. Jacques R. Nicoli
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  8. Maria I. T. D. Correia
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  9. Valbert N. Cardoso
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Corresponding author

Correspondence to Valbert N. Cardoso.

Additional information

Communicated by Erko Stackebrandt.

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Generoso, S.V., Viana, M., Santos, R. et al. Saccharomyces cerevisiae strain UFMG 905 protects against bacterial translocation, preserves gut barrier integrity and stimulates the immune system in a murine intestinal obstruction model. Arch Microbiol 192, 477–484 (2010). https://doi.org/10.1007/s00203-010-0574-8

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  • DOI: https://doi.org/10.1007/s00203-010-0574-8

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