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Effects of the antibiotic growth promoters flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

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Abstract

The 16S rDNA PCR-DGGE and rpoB quantitative PCR (RQ-PCR) techniques were used to evaluate the effects of dietary flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia. The fish were fed four diets: control, dietary flavomycin, florfenicol and their combination. After 8 weeks of feeding, 6 fish from each cage were randomly chosen for the analysis. The total number of intestinal bacteria was determined by RQ-PCR. The results showed that dietary antibiotics significantly influenced the intestinal microbiota and dramatically reduced the intensity of total intestinal bacterial counts. The intensity of some phylotypes (EU563257, EU563262 and EU563255) were reduced to non-detectable levels by both dietary antibiotics, while supplementation of florfenicol to the diet also reduced the intensity of the phylotypes EU563242 and EU563262, uncultured Mycobacterium sp.-like, uncultured Cyanobacterium-like and uncultured Cyanobacterium (EU563246). Dietary flavomycin only reduced the OTU intensity of one phylotype, identified as a member of the phylum Fusobacteria. The antibiotic combination only reduced the phylotypes EU563242 and EU563262. Based on our results, we conclude that the reduced effect of florfenicol on intestinal microbiota was stronger than that of flavomycin, and when flavomycin and florfenicol were added in combination, the effect of florfenicol overshadowed that of flavomycin.

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Acknowledgments

This research was supported by the National High Technology Research and Development Program of China (863 Program; grant 2007AA100601) and the Key Program of Transgenic Plant Breeding (2009ZX08003-020B).

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Authors and Affiliations

  1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China

    Suxu He, Zhigang Zhou, Yuchun Liu, Yanan Cao, Kun Meng, Pengjun Shi & Bin Yao

  2. Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø, 9037, Tromsø, Norway

    Einar Ringø

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  1. Suxu He
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Correspondence to Zhigang Zhou or Bin Yao.

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Communicated by Shuang-Jiang Liu.

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He, S., Zhou, Z., Liu, Y. et al. Effects of the antibiotic growth promoters flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia (Oreochromis niloticus ♀ × O. aureus ♂). Arch Microbiol 192, 985–994 (2010). https://doi.org/10.1007/s00203-010-0627-z

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