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Genotoxic effect of caffeine in Yarrowia lipolytica cells deficient in DNA repair mechanisms

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Abstract

Caffeine is a compound that can exert physiological–beneficial effects in the organism. Nevertheless, there are controversies about its protective-antioxidant and/or its negative genotoxic effect. To abound on the analysis of the possible genotoxic/antioxidant effect of caffeine, we used as research model the yeast Yarrowia lipolytica parental strain, and mutant strains (∆rad52 and ∆ku80), which are deficient in the DNA repair mechanisms. Caffeine (5 mM) showed a cytostatic effect on all strains, but after 72 h of incubation the parental and ∆ku80 strains were able to recover of this inhibitory effect on growth, whereas ∆rad52 was unable to recover. When cells were pre-incubated with caffeine and H2O2 or incubated with a mixture of both agents, a higher inhibitory effect on growth of mutant strains was observed and this effect was noticeably greater for the Δrad52 strain. The toxic effect of caffeine appears to be through a mechanism of DNA damage (genotoxic effect) that involves DSB generation since, in all tested conditions, the growth of Δrad52 strain (cells deficient in HR DNA repair mechanism) was more severely affected.

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Funding

This work was supported in part by National Council of Science and Technology of México (CONACYT) with a fellowship to CAQG (Grant No. 581423), and the UANL-PAICYT Program under Grant No. CS1166-11.

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Authors and Affiliations

  1. Universidad Autónoma de Nuevo León, Centro de Investigación en Nutrición y Salud Pública, Av. Dr. Eduardo Aguirre Pequeño/Yuriria, Col. Mitras Centro, 64460, Monterrey, Nuevo León, Mexico

    Clara A. Quiñones-González, Gerardo I. Arredondo-Mendoza, Zacarías Jiménez-Salas & Eduardo Campos-Góngora

  2. Departamento de Ciencias Biomédicas, Área Microbiología, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain

    Germán Larriba-Calle

  3. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    José Ruiz-Herrera

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  1. Clara A. Quiñones-González
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  2. Gerardo I. Arredondo-Mendoza
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  3. Zacarías Jiménez-Salas
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  5. José Ruiz-Herrera
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  6. Eduardo Campos-Góngora
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Correspondence to Eduardo Campos-Góngora.

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Communicated by Erko Stackebrandt.

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Quiñones-González, C.A., Arredondo-Mendoza, G.I., Jiménez-Salas, Z. et al. Genotoxic effect of caffeine in Yarrowia lipolytica cells deficient in DNA repair mechanisms. Arch Microbiol 201, 991–998 (2019). https://doi.org/10.1007/s00203-019-01658-4

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  • DOI: https://doi.org/10.1007/s00203-019-01658-4

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