Abstract
A Gram-stain-negative, aerobic, non-motile, yellow-pigmented rod-shaped and alginate-degrading bacterium, designated B1N29T, was isolated from the gut of the abalone Haliotis rubra obtained in Weihai, China. Strain B1N29T was found to grow at 4–35 ℃ (optimum, 25 ℃), at pH 6.5–9.0 (optimum, 7.0–7.5) and in the presence of 0.5–9% (w/v) NaCl (optimum, 2%). Cells were positive for oxidase and catalase activity. The 16S rRNA-based phylogenetic analysis revealed that the nearest phylogenetic neighbors of strain B1N29T were Tamlana carrageenivorans KCTC 62451T (98.2%) and Tamlana agarivorans KCTC 22176T (97.7%). Based on the phylogenomic analysis, the average nucleotide identity (ANI) values between strain B1N29T and the neighbor strains were 79.2 and 79.0%, respectively; the digital DNA–DNA hybridization (dDDH) values between strain B1N29T and its two closest neighbors were 22.8 and 23.0%, respectively. Menaquinone-6 (MK-6) was detected as the sole respiratory quinone. The dominant cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, anteiso-C15:0 and iso-C15:1 G. The polar lipids included phosphatidylethanolamine, one aminophospholipid, seven aminolipids and five unidentified lipids. Based on the phylogenetic and phenotypic characteristics, strain B1N29T is considered to represent a novel species of the genus Tamlana, for which the name Tamlana haliotis sp. nov. is proposed. The type strain is B1N29T (= KCTC 72683T = MCCC 1H00394T).
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Acknowledgements
Authors thank Qi-Yun Liang for the work of phylogenomic analysis.
Funding
This work was supported by the Natural Science Foundation of Shandong Province, China (No. ZR2020MD088), the Open Fund of the Key Laboratory of Marine Geology and Environment, Chinese Academy of Sciences (No. MGE2020KG14), the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDB42000000), State Key Laboratory of Microbial Technology Open Projects Fund (No. M2020-03) and the National Natural Science Foundation of China (No. 41976202).
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Strain B1N29T was isolated by BTL. Material preparation, data collection and analysis were performed by WRC, BTL, XKS, YYS and MYJ. The manuscript was written by WRC. Project guidance and critical revision of manuscripts were performed by ZJD. All authors read and approved the final manuscript.
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Communicated by Erko Stackebrandt.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain B1N29T is MN519996. The GenBank/EMBL/DDBJ accession number for the genome sequences of strain B1N29T is WAAT00000000.
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Cao, WR., Liu, BT., Sun, XK. et al. Tamlana haliotis sp. nov., isolated from the gut of the abalone Haliotis rubra. Arch Microbiol 203, 2357–2364 (2021). https://doi.org/10.1007/s00203-021-02216-7
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DOI: https://doi.org/10.1007/s00203-021-02216-7