Skip to main content
SpringerLink
Log in

Protective effects of dioscin against alcohol-induced liver injury

  • Organ Toxicity and Mechanisms
  • Published:
Archives of Toxicology Aims and scope Submit manuscript

A Correction to this article was published on 29 August 2019

This article has been updated

Abstract

Our previous studies have shown that dioscin has protective effect against liver injury. However, the action of the compound against ethanol-induced liver injury is still unknown. In the present paper, ethanol-induced acute and chronic liver damage rat models were used, and the results showed that dioscin significantly alleviated liver steatosis, reduced the levels of alanine aminotransferase, aspartate aminotransferase, total triglyceride (TG), total cholesterol and malondialdehyde, and increased the levels of high-density lipoprotein, superoxide dismutase, glutathione and glutathione peroxidase. Transmission electron microscopy and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assays showed that dioscin prevented mitochondrial ultrastructural alterations and apoptosis caused by ethanol. In addition, dioscin significantly inhibited ethanol-induced cytochrome P450 2E1 activation, down-regulated the levels of mitogen-activated protein kinases phosphorylation, inhibited the expressions of nuclear factor kappa B, glucose regulated protein 78, activating transcription factor 6 and alpha subunit of translation initiation factor 2 to attenuate oxidative damage, decreased the expressions of tumor necrosis factor alpha and interleukin-6, and down-regulated the expressions of apoptosis-related proteins including p53, caspase-3, caspase-9, poly (ADP-ribose)-polymerase and cytokeratin-18. Further investigation indicated that dioscin markedly increased the expressions of peroxisome proliferators-activated receptor α and its target genes including medium-chain acyl-CoA dehydrogenase, carnitine palmitoyl-CoA transferase I and acyl-CoA oxidase to advance fatty acid β-oxidation, up-regulated the expressions of acyl-CoA synthetase long-chain family member 1, acyl-CoA synthetase long-chain family member 5, alpha-aminoadipic semialdehyde dehydrogenase and acyl-CoA dehydrogenase to promote fatty acid metabolism, and down-regulated the expressions of glycerol-3-phosphate acyltransferase, diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 to accelerate TG synthesis. However, dioscin had no effects on the expressions of sterol regulatory element-binding protein-1c, fatty acid synthase, acetyl-CoA carboxylase 1 and stearoyl-CoA desaturase 1 associated with fatty acid synthesis. In conclusion, dioscin shows excellent protective effect against ethanol-induced liver injury through ameliorating ethanol-induced oxidative stress, mitochondrial function, inflammatory cytokine production, apoptosis and liver steatosis, which should be developed as a new drug for the treatment of ethanol-induced liver injury in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Change history

  • 29 August 2019

    During the course of writing and revision of this paper, the band of GAPDH.

  • 29 August 2019

    During the course of writing and revision of this paper, the band of GAPDH.

Abbreviations

APAP:

Acetaminophen

CCl4 :

Carbon tetrachloride

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

TC:

Total cholesterol

TG:

Total triglyceride

MDA:

Malondialdehyde

HDL:

High-density lipoprotein

SOD:

Superoxide dismutase

GSH:

Glutathione

GSH-Px:

Glutathione peroxidase

GSR:

Glutathione reductase

Ct:

Threshold cycle

CYP2E1:

Cytochrome P450 2E1

ROS:

Reactive oxygen species

MAPKs:

Mitogen-activated protein kinases

NF-κB:

Nuclear factor kappa B

GRP78:

Glucose regulated protein 78

ATF6:

Activating transcription factor 6

EIF2:

Alpha subunit of translation initiation factor 2

TNF-α:

Tumor necrosis factor alpha

IL-6:

Interleukin-6

PARP:

Poly (ADP-ribose)-polymerase

TEM:

Transmission electron microscopy

TUNEL:

Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling

PPAR-α:

Peroxisome proliferators-activated receptor α

MCAD:

Medium-chain acyl-CoA dehydrogenase

CPT I:

Carnitine palmitoyl-CoA transferase I

ACO:

Acyl-CoA oxidase

ACSL1:

Acyl-CoA synthetase long-chain family member 1

ACSL5:

Acyl-CoA synthetase long-chain family member 5

ALDH7A1:

Alpha-aminoadipic semialdehyde dehydrogenase

ACADS:

Acyl-CoA dehydrogenase

GAPT:

Glycerol-3-phosphate acyltransferase

DAGT1:

Diacylglycerol acyltransferase 1

DAGT2:

Diacylglycerol acyltransferase 2

SREBP-1c:

Sterol regulatory element-binding protein-1c

FAS:

Fatty acid synthase

ACC1:

Acetyl-CoA carboxylase 1

SCD1:

Stearoyl-CoA desaturase 1

References

  • Ajakaiye MA, Jacob A, Wu R, Zhou M, Ji Y, Dong W, Wang Z, Qiang X, Chaung WW, Nicastro J, Coppa GF, Wang P (2011) Upregulation of Kupffer cell α2A- Adrenoceptors and down regulation of MKP-1 mediate hepatic injury in chronic alcohol exposure. Biochem Biophys Res Commun 409:406–411

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Aroor AR, James TT, Jackson DE, Shukla SD (2010) Differential changes in MAP kinases, histone modifications, and liver injury in rats acutely treated with ethanol. Alcohol Clin Exp Res 34:1543–1551

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Arteel GE (2003) Oxidant and antioxidant in alcohol-induced liver disease. Gastroenterologia 124:778–790

    Article  CAS  Google Scholar 

  • Balunas MJ, Kinghorn AD (2005) Drug discovery from medicinal plants. Life Sci 78:431–441

    Article  CAS  PubMed  Google Scholar 

  • Bin G, Ramon B (2011) Alcoholic liver disease: pathogenesis and new therapeutic targets. Gastroenterology 141:1572–1585

    Article  Google Scholar 

  • Brocker C, Lassen N, Estey T et al (2010) Aldehyde dehydrogenase 7A1 (ALDH7-A1) is a novel enzyme involved in cellular defense against hyperosmotic Stress. J Biol Chem 285:18452–18463

    Article  CAS  PubMed  Google Scholar 

  • Butler JA, Hagen TM, Moreau R (2009) Lipoic acid improves hypertriglyceridemia by stimulating triacylglycerol clearance and downregulating liver triacylglycerol secretion. Arch Biochem Biophys 485:63–71

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Cai L (2008) Alcoholic cardiomyopathy: acetaldehyde, insulin insensitization and ER stress. J Mol Cell Cardiol 44:979–982

    Article  CAS  PubMed  Google Scholar 

  • Cao Q, Mak KM, Lieber CS (2005) Cytochrome P4502E1 primes macrophages to increase TNF-alpha production in response to liposaccharide. Am J Physiol 289:G95–G107

    Article  CAS  Google Scholar 

  • Cave M, Deaciuc I, Mendez C, Song Z, Joshi-Barve S, Barve S, McClain C (2007) Nonalcohol fatty liver disease: predisposing factors and the role of nutrition. J Nutr Biochem 18:184–195

    Article  CAS  PubMed  Google Scholar 

  • Cherian PP, Schenker S, Henderson GI (2008) Ethanol-mediated DNA damage and PARP-1 apoptotic responses in cultured fetal cortical neurons. Alcohol Clin Exp Res 32:1884–1892

    CAS  PubMed Central  PubMed  Google Scholar 

  • Day CP (2006) Genes or environment to determine alcoholic liver disease and nonalcoholic fatty liver disease. Liver Int 26:1021–1028

    Article  CAS  PubMed  Google Scholar 

  • Derdak Z, Lang CH, Villegas KA, Tong M, Mark NM, de la Monte SM, Wands JR (2011) Activation of p53 enhances apoptosis and insulin resistance in a rat model of alcoholic liver disease. J Hepatol 1:164–172

    Article  Google Scholar 

  • Ding RB, Tian K, Huang LL, He CW, Jiang Y, Wang YT, Wan JB (2012) Herbal medicines for the prevention of alcoholic liver disease: a review. J Ethnopharmacol 144:457–465

    Article  PubMed  Google Scholar 

  • Dong H, Lu F, Zhao L (2012) Chinese herbal medicine in the treatment of nonalcoholic fatty liver disease. Chinese. J Integr Med 18:152–160

    Google Scholar 

  • Emanuele A (2008) Oxidative mechanisms in the pathogenesis of alcoholic liver disease. Mol Aspects Med 29:9–16

    Article  Google Scholar 

  • Gavin A, Luis M, Christian M, Frederick B, Craig JM (2003) Advances in alcoholic liver disease. Best Pract Res Clin Gastroenterol 17:625–647

    Article  Google Scholar 

  • Giovanni M, Roberto G, Maurizio C (2009) Recent insights into hepatic lipid metabolism in non-alcoholic fatty liver disease (NAFLD). Prog Lipid Res 48:1–26

    Article  Google Scholar 

  • Gnoni GV, Paglialonga G, Siculella L (2009) Quercetin inhibits fatty acid and triacylglycerol synthesis in rat-liver cells. Eur J Clin Invest 39:761–768

    Article  CAS  PubMed  Google Scholar 

  • Herbert T, Moschen AR, Kaneider NC (2011) Pathways of liver injury in alcoholic liver disease. J Hepatol 55:1159–1161

    Article  Google Scholar 

  • Hines IN, Wheeler MD (2004) Recent advances in alcoholic liver disease III. Role of the innate immune response in alcoholic hepatitis. Am J Physiol 287:G310–G314

    Article  CAS  Google Scholar 

  • Hoek JB, Pastorino JG (2002) Ethanol, oxidative stress, and cytokine-induced liver cell injury. Alcohol 27:63–68

    Article  CAS  PubMed  Google Scholar 

  • Hsieh MJ, Tsai TL, Hsieh YS, Wang CJ, Chiou HL (2013) Dioscin-induced autophagy mitigates cell apoptosis through modulation of PI3 K/Akt and ERK and JNK signaling pathways in human lung cancer cell lines. Arch Toxicol In Press

  • Hu MM, Xu LN, Yin LH, Xu Y, Han X, Peng J, Wan X (2013) Cytotoxicity of dioscin in human gastric carcinoma cells through death receptor and mitochondrial pathways. J Appl Toxicol 33:712–722

    Article  CAS  PubMed  Google Scholar 

  • Jessica IC, Sanjoy R, Patricia MD, Jacobsen DW, Nagy LE (2009) Exogenous thioredoxin prevents ethanol-induced oxidative damage and apoptosis in mouse liver. Hepatology 49:1709–1719

    Article  Google Scholar 

  • Ji C, Kaplowitz N (2003) Betaine decreases hyperhomocysteinemia, endoplasmic reticulum stress, and liver injury in alcohol-fed mice. Gastroenterology 124:1488–1499

    Article  CAS  PubMed  Google Scholar 

  • Knight BL, Hebbachi A, Hauton D et al (2005) A role for PPAR-α in the control of SREBP activity and lipid synthesis in the liver. Biochem J 389:413–421

    Article  CAS  PubMed  Google Scholar 

  • Kohjima M, Enjoji M, Higuchi N et al (2007) Revaluation of fatty acid metabolism-related gene expression in nonalcoholic fatty liver disease. Int J Mol Med 20:351–358

    CAS  PubMed  Google Scholar 

  • Laurent M, Etienne B, Beatrice L et al (2011) ATF4 and the integrated stress response are induced by ethanol and cytochrome P450 2E1 in human hepatocytes. J Hepatol 54:729–737

    Article  Google Scholar 

  • Leung TM, Nieto N (2013) CYP2E1 and oxidant stress in alcoholic and non- alcoholic fatty liver disease. J Hepatol 58:395–398

    Article  CAS  PubMed  Google Scholar 

  • Li SY, Ren J (2008) Cardiac overexpression of alcohol dehydrogenase exacerbates chronic ethanol ingestion-induced myocardial dysfunction and hypertrophy: role of insulin signaling and ER stress. J Mol Cell Cardiol 44:992–1001

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Li M, Han XW, Yu B (2003) Synthesis of monomethylated dioscin derivatives and their antitumor activities. Carbohydr Res 338:117–121

    Article  CAS  PubMed  Google Scholar 

  • Li H, Huang W, Wen YQ, Gong GH, Zhao QB, Yu G (2010) Anti-thrombotic activity and chemical characterization of steroidal saponins from Dioscorea zingiberensis C.H. Wright. Fitoterapia 81:1147–1156

    Article  CAS  PubMed  Google Scholar 

  • Lieber CS (2000) Alcoholic liver disease: new insights in pathogenesis lead to new treatments. J Hepatol 32:113–128

    Article  CAS  PubMed  Google Scholar 

  • Lu Y, Cederbaum AI (2008) CYP2E1 and oxidative liver injury by alcohol. Free Radic Biol Med 44:723–738

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lu BN, Yin LH, Xu LN, Peng JY (2011) Application of proteomic and bioinformatic techniques for studying the hepatoprotective effect of dioscin against CCl4-induced liver damage in mice. Planta Med 77:407–415

    Article  CAS  PubMed  Google Scholar 

  • Lu BN, Xu YS, Xu LN et al (2012) Mechanism investigation of dioscin against CCl4- induced acute liver damage in mice. Environ Toxicol Pharmacol 34:127–135

    Article  CAS  PubMed  Google Scholar 

  • Lykkesfeldt J (2007) Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clin Chim Acta 380:50–58

    Article  CAS  PubMed  Google Scholar 

  • Mandrekar P, Szabo G (2009) Signalling pathways in alcohol-induced liver inflammation. J Hepatol 50:1258–1266

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Matsubara Y, Indo Y, Naito E, Ozasa H et al (1989) Molecular cloning and nucleotide sequence of cDNAs encoding the precursors of rat long chain acyl-coenzyme A, short chain acyl-coenzyme A, and isovaleryl-coenzyme A dehydrogenases. Sequence homology of four enzymes of the acyl-CoA dehydrogenase family. J Biol Chem 264:16321–16331

    CAS  PubMed  Google Scholar 

  • McVicker BL, Tuma DJ, Kubik JL, Tuma PL, Casey CA (2006) Ethanol-induced apoptosis in polarized hepatic cells possibly through regulation of the Fas pathway. Alcohol Clin Exp Res 30:1906–1915

    Article  CAS  PubMed  Google Scholar 

  • Moriya T, Naito H, Ito Y, Nakajima T (2009) Hypothesis of seven balances: molecular mechanisms behind alcoholic liver diseases and association with PPAR alpha. J Occup Health 51:391–403

    Article  CAS  PubMed  Google Scholar 

  • Nagy LE (2003) Recent insights into the role of the innate immune system in the development of alcoholic liver disease. Exp Biol Med 228:882–890

    CAS  Google Scholar 

  • Nakajima T, Kamijo Y, Tanaka N et al (2004) Peroxisome proliferator-activated receptor alpha protects against alcohol-induced liver damage. Hepatology 40:972–980

    Article  CAS  PubMed  Google Scholar 

  • Nakamura T, Komori C, Lee Y et al (1996) Cytotoxic activities of solanum steroidal glycosides. Biol Pharm Bull 19:564–566

    Article  CAS  PubMed  Google Scholar 

  • Nanji AA, Jokelainen K, Tipoe GL, Rahemtulla A, Thomas P, Dannenberg AJ (2003) Curcumin prevents alcohol-induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes. Am J Physiol Gastrointest Liver Physiol 284:G321–G327

    CAS  PubMed  Google Scholar 

  • Pai JT, Guryev O, Brown MS, Goldstein JL (1998) Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins. J Biol Chem 273:26138–26148

    Article  CAS  PubMed  Google Scholar 

  • Pana TL, Wanga PW, Leub YL, Wuc TH, Wud TS (2012) Inhibitory effects of Scutellaria baicalensis extract on hepatic stellate cells through inducing G2/M cell cycle arrest and activating ERK-dependent apoptosis via Bax and caspase pathway. J Ethnopharmacol 139:829–837

    Article  Google Scholar 

  • Pani G, Fusco S, Colavitti R, Borrello S, Maggiano N, Cravero AA (2004) Abrogation of hepatocyte apoptosis and early appearance of liver dysplasia in ethanol-fed p53-deficient mice. Biochem Biophys Res Commun 325:97–100

    Article  CAS  PubMed  Google Scholar 

  • Pari L, Karthikesan K (2007) Protective role of caffeic acid against alcohol-induced biochemical changes in rats. Fundam Clin Pharmacol 21:355–361

    Article  CAS  PubMed  Google Scholar 

  • Powel EE, Jonsson JR, Clouston AD (2005) Steatosis: co-factor in other liver diseases. Hepatology 42:5–13

    Article  Google Scholar 

  • Raj MD (2011) Adverse effects of corticosteroids on bone metabolism: a review. Phys Med Rehab 3:466–471

    Google Scholar 

  • Ramsay RR, Richard DG, Feike RL (2001) Molecular enzymology of carnitine transfer and transport. Biochim Biophys Acta 1546:21–43

    Article  CAS  PubMed  Google Scholar 

  • Rehm J, Mathers C, Popova S, Thavorncharoensap M, Teerawattananon Y, Patra J (2009) Global burden of disease and injury and economic cost attributable to alcohol use and alcohol-use disorders. Lancet 373:2223–2233

    Article  PubMed  Google Scholar 

  • Rosa LC, Moshagel H, Nieto N (2008) Hepatocyte oxidant stress and alcoholic liver disease. Rev Esp Enferm Dig 100:156–163

    Google Scholar 

  • Schoonjans K, Staels B, Grimaldi P, Auwerx J (1993) Acyl-CoA synthetase mRNA expression is controlled by fibric-acid derivatives, feeding and liver proliferation. Eur J Biochem 216:615–622

    Article  CAS  PubMed  Google Scholar 

  • Seth D, Haber PS, Syn WK, Diehl AM, Day CP (2011) Pathogenesis of alcohol-induced liver disease: classical concepts and recent advances. J Gastroenterol Hepatol 26:1089–1105

    Article  CAS  PubMed  Google Scholar 

  • Shen Z, Liang X, Rogers CQ, Rideout D, You M (2010) Involvement of adiponectin -SIRT1-AMPK signaling in the protective action of rosiglitazone against alcoholic fatty liver in mice. Am J Physiol Gastrointest Liver Physiol 298:364–374

    Article  Google Scholar 

  • Shimano H, Horton JD, Shimomura I, Hammer RE, Brown MS, Goldstein JL (1997) Isoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J Clin Invest 99:846–854

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Song BJ, Moon KH, Olsson NU, Salem JN (2008) Prevention of alcoholic fatty liver and mitochondrial dysfunction in the rat by long-chain polyunsaturated fatty acids. J Hepatol 49:262–273

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Stewart SF, Day CP (2003) The management of alcoholic liver disease. J Hepatol 38:S2–S13

    Article  PubMed  Google Scholar 

  • Thakur V, Pritchard MT, McMullen MR, Wang Q, Nagy LE (2006) Chronic ethanol feeding increases activation of NADPH oxidase by liposaccharide in rat Kuper cells: role of increased reactive oxygen in LPS-stimulated ERK1/2 activation and TNF-alpha production. J Leukocyte Biol 79:1348–1356

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • VanGuilder HD, Vrana KE, Freeman WM (2008) Twenty-five years of quantitative PCR for gene expression analysis. Biotechniques 44:619–626

    Article  CAS  PubMed  Google Scholar 

  • Wagner M, Zollner G, Trauner M (2011) Nuclear receptors in liver disease. Hepatology 53:1023–1034

    Article  CAS  PubMed  Google Scholar 

  • Wieckowska A, Zein NN, Yerian LM, Lopez AR, McCullough AJ, Feldstein AE (2006) In vivo assessment of liver cell apoptosis as a novel biomarker of disease severity in nonalcoholic fatty liver disease. Hepatology 44:27–33

    Article  CAS  PubMed  Google Scholar 

  • Xiong Y, Yang YQ, Yang J et al (2010) Tectoridin, an isoflavone glycoside from the flower of Pueraria lobata, prevents acute ethanol-induced liver steatosis in mice. Toxicology 276:64–72

    Article  CAS  PubMed  Google Scholar 

  • Xu TT, Zhang S, Zheng LL, Yin LH, Xu LN, Peng JY (2012) A 90-day subchronic toxicological assessment of dioscin, a natural steroid saponin, in Sprague–Dawley rats. Food Chem Toxicol 50:1279–1287

    Article  CAS  PubMed  Google Scholar 

  • Yang MY, Peng CH, Chan KC, Yang YS, Huang CN, Wang CJ (2010) The hypolipidemic effect of Hibiscus sabdariffa polyphenols via inhibiting lipogenesis and promoting hepatic lipid clearance. J Agric Food Chem 58:850–859

    Article  CAS  PubMed  Google Scholar 

  • Yin HQ, Kim YC, Chung YS, Kim YC, Shin YK, Lee BH (2009) Honokiol reverses alcoholic fatty liver by inhibiting the maturation of sterol regulatory element binding protein-1c and the expression of its downstream lipogenesis genes. Toxicol Appl Pharmacol 236:124–130

    Article  CAS  PubMed  Google Scholar 

  • Yin LH, Xu LN, Wang XN, Lu BN, Liu YT, Peng JY (2010a) An economical method for isolation of dioscin from Dioscorea nipponica Makino by HSCCC coupled with ELSD, and a computer-aided UNIFAC mathematical model. Chromatographia 71:15–23

    Article  CAS  Google Scholar 

  • Yin LH, Xu YS, Xu LN, Qi Y, Han X, Peng JY (2010b) A green and efficient protocol for industrial-scale preparation of dioscin from Dioscorea nipponica Makino by two-step macroporous resin column chromatography. Chem Eng J 165:281–289

    Article  CAS  Google Scholar 

  • Yip WW, Burt AD (2006) Alcoholic liver disease. Semin Diagn Pathol 23:149–160

    Article  PubMed  Google Scholar 

  • Yu S, Rao S, Reddy JK (2003) Peroxisome proliferator-activated receptors, fatty acid oxidation, steatohepatitis and hepatocarcinogenesis. Curr Mol Med 3:561–572

    Article  CAS  PubMed  Google Scholar 

  • Zeng T, Xie KQ (2009) Ethanol and liver: recent advances in the mechanisms of ethanol-induced hepatosteatosis. Arch Toxicol 83:1075–1081

    Article  CAS  PubMed  Google Scholar 

  • Zhao XM, Cong XN, Zheng LL, Xu LN, Yin LH, Peng JY (2012) Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo. Toxicol Lett 214:69–80

    Article  CAS  PubMed  Google Scholar 

  • Zoltan D, Charles HL, Kristine AV, Ming T, Nicholas MM, Suzanne MM, Jack RW (2011) Activation of p53 enhances apoptosis and insulin resistance in a rat model of alcoholic liver disease. J Hepatol 54:164–172

    Article  Google Scholar 

Download references

Acknowledgments

This work was financially supported by National Natural Science Foundation of China (No. 81274195) and the Program for New Century Excellent Talents in University (NCET-11-1007).

Conflict of interest

None.

Author information

Authors and Affiliations

  1. College of Pharmacy, Dalian Medical University, 9 Western Lvshun South Road, Dalian, 116044, China

    Tingting Xu, Lina Xu, Lianhong Yin, Yan Qi, Youwei Xu, Xu Han & Jinyong Peng

  2. Department of Pharmaceuticals, The First Affiliated Hospital, Dalian Medical University, Dalian, 116011, China

    Lingli Zheng

  3. Research Institute of Integrated Traditional and Western Medicine of Dalian Medical University, Dalian, 116011, China

    Jinyong Peng

Authors
  1. Tingting Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lingli Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lina Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lianhong Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yan Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Youwei Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xu Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jinyong Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jinyong Peng.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 863 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, T., Zheng, L., Xu, L. et al. Protective effects of dioscin against alcohol-induced liver injury. Arch Toxicol 88, 739–753 (2014). https://doi.org/10.1007/s00204-013-1148-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00204-013-1148-8

Keywords

Search

Navigation