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Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes

  • Inorganic Compounds
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Abstract

Previous studies have reported that excessive fluoride exposure induced liver damage. However, the underlying mechanism of fluoride-induced hepatic toxicity is still unclear. Hence, this study was aimed to evaluate the fluoride-induced apoptosis, autophagy, and IL-17 signaling pathway-related genes to explore the possible mechanisms of NaF-induced liver injury in mice. For this, 48 male mice were allotted randomly to four groups, treated with deionized water, 25, 50, 100 mg/L NaF for 150 days continuously. Our results suggested that treatment with NaF decreased the PAS staining-positive area, with a concomitant increase in liver score, and serum ALT and AST levels which indicated that NaF induced the liver injury. In addition, the qRT-PCR, immunohistochemistry, and western blotting results indicated that NaF exposure activated IL-17 signaling, apoptosis, and autophagy pathways. In summary, these results suggested that NaF induced apoptosis and autophagy in liver by activating the IL-17 signaling pathway, eventually leading to impaired liver function.

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Acknowledgements

This work is supported by the National Natural Science Foundation (Grant nos. 31672623, 31372497) of China and Veterinary Environmental Lab (Shanxi Key Lab).

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  1. Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, China

    Yangfei Zhao, Yanyan Li, Jinming Wang, Ram Kumar Manthari & Jundong Wang

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  1. Yangfei Zhao
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  2. Yanyan Li
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Correspondence to Jundong Wang.

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Zhao, Y., Li, Y., Wang, J. et al. Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes. Arch Toxicol 92, 3277–3289 (2018). https://doi.org/10.1007/s00204-018-2305-x

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