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Effect of naloxone and morphine on arcaine-induced state-dependent memory in rats

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Abstract

Rationale

Arcaine is a competitive antagonist of the polyamine binding site at the N-methyl-D-aspartic acid receptor which induces state-dependent recall. However, no study has addressed the involvement of other neurotransmitter/neuromodulators in arcaine-induced state dependency.

Objectives

The current study investigates whether the opioid system is involved in arcaine-induced state-dependent memory retrieval of the inhibitory avoidance task (IA) in rats.

Results

The systemic administration of arcaine (30 mg/kg, intraperitoneally (i.p.)) or morphine (5 mg/kg, i.p.) 0, 3, 6, or 9 h post-training, reduced step-down latencies at testing. Arcaine (30 mg/kg, i.p.) or morphine (5 mg/kg, i.p.) injection 30 min before testing reversed the performance deficit induced by administration of arcaine or morphine 0, 3 or 6, but not 9 h post-training. The reversal of arcaine-induced impairment of IA performance was completely transferred to morphine and vice versa. The association of low and ineffective doses of morphine and arcaine (10 and 1.5 mg/kg, respectively) were additive and caused state dependency. Naloxone (2 mg/kg, 3 min post-training, or 1 mg/kg, 1 h pre-test, i.p.) reversed the amnesia and the state dependency induced by morphine and arcaine.

Conclusion

These results suggest that state dependency induced by arcaine involves the opioid system.

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Acknowledgments

This study was supported by CNPq and CAPES. All the experiments comply with the current laws of Brazil.

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Authors and Affiliations

  1. Laboratório de Neurotoxicidade e Psicofarmacologia, Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, 97105-900, Rio Grande do Sul, Brazil

    Raquele Kipper Mariani, Michelle Melgarejo Rosa, Ana Paula Chiapinotto Ceretta, Keli Camera & Maribel Antonello Rubin

  2. Laboratório de Neurotoxicidade e Psicofarmacologia, Departamento de Fisiologia e Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, 97105-900, Rio Grande do Sul, Brazil

    Carlos Fernando Mello

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  1. Raquele Kipper Mariani
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  2. Carlos Fernando Mello
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  4. Ana Paula Chiapinotto Ceretta
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  5. Keli Camera
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Corresponding author

Correspondence to Maribel Antonello Rubin.

Electronic supplementary materials

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Supplemental Table S1

Effect of treatments of experiment 1 on the training step-down latency (s) and on the behavior of rats in the open field immediately after the inhibitory avoidance testing session (DOC 46 kb)

Supplemental Table S2

Effect of treatments of experiment 1 on the training step-down latency (s) and on the behavior of rats in the open field immediately after the inhibitory avoidance testing session (DOC 48 kb)

Supplemental Table S3

Effect of treatments of experiment 2 on the training step-down latency (s) and on the behavior of rats in the open field immediately after the inhibitory avoidance testing session (DOC 36 kb)

Supplemental Table S4

Effect of treatments of experiment 3 on the training step-down latency (s) and on the behavior of rats in the open field immediately after the inhibitory avoidance testing session (DOC 46 kb)

Supplemental Table S5

Effect of treatments of experiment 4 on the training step-down latency (s) and on the behavior of rats in the open field immediately after the inhibitory avoidance testing session (DOC 45 kb)

Supplemental Table S6

Effect of arcaine (30 mg/kg) or vehicle (Sal) administration immediately post-training on the test step-down latency (s) of rats pseudo trained (without shock) (DOC 28 kb)

Supplemental Table S7

Effect of vehicle (Sal) administration immediately post-training and 30 min pre-test on the training and test step-down latency (s) (DOC 28 kb)

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Mariani, R.K., Mello, C.F., Rosa, M.M. et al. Effect of naloxone and morphine on arcaine-induced state-dependent memory in rats. Psychopharmacology 215, 483–491 (2011). https://doi.org/10.1007/s00213-011-2215-6

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