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Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode

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Abstract

A nanostructured film electrode, a multi-wall carbon nanotubes (MWNT)-modified glassy carbon electrode (GCE), is described for the simultaneous determination of guanine and adenine. The properties of the MWNT-modified GCE were investigated by scanning electron microscopy (SEM) and cyclic voltammetry. The oxidation peak currents of guanine and adenine increased significantly at the MWNT-modified GCE in contrast to those at the bare GCE. The experimental parameters were optimized and a direct electrochemical method for the simultaneous determination of guanine and adenine was proposed. Using the MWNT-modified GCE, a sensitive and direct electrochemical technique for the measurement of native DNA was also developed, and the value of (G+C)/(A+T) of HCl-digested DNA was detected.

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Abbreviations

MWNT:

Multi-wall carbon nanotubes

G:

Guanine

A:

Adenine

GCE:

Glassy carbon electrode

DHP:

Dihexadecyl hydrogen phosphate

SEM:

Scanning electron microscope

References

  1. Paleček E (1960) Nature 188:656

    Google Scholar 

  2. Millan KM, Mikkelsen SR (1993) Anal Chem 65:2317

    CAS  PubMed  Google Scholar 

  3. Takenaka S, Yamashita K, Takagi M, Uto Y, Konda H (2000) Anal Chem 72:1334

    Article  CAS  Google Scholar 

  4. Yu CJ, Wan YJ, Yowanto H, Li J, Tao CL, James MD, Tan CL, Blackburn GF, Meade TJ (2001) J Am Chem Soc 123:11155

    Article  CAS  PubMed  Google Scholar 

  5. Erdem A, Kerman K, Meric B, Ozsoz M (2001) Electroanalysis 13:119

    Article  Google Scholar 

  6. Paleček E (1996) Electroanalysis 8:7

    CAS  Google Scholar 

  7. Singhal P, Kuhr WG (1997) Anal Chem 69:4828

    Article  CAS  PubMed  Google Scholar 

  8. Wang J, Cai XH, Wang JY, Jonsson C (1995) Anal Chem 67:4065

    CAS  Google Scholar 

  9. Wang J, Rivas G, Ozsoz M, Grabt DH, Cai XH, Parrado C (1997) Anal Chem 69:1457

    CAS  Google Scholar 

  10. De-los-Santos-Álvarez P, Lobo-Castañón MJ, Miranda-Ordieres AJ, Tuñón-Blanco P (2002) Anal Chem 74:3342

    Article  PubMed  Google Scholar 

  11. Jelen F, Yosypchuk B, Kourilová A, Novotný L, Paleček E (2002) Anal Chem 74:4788

    Article  CAS  PubMed  Google Scholar 

  12. Gilmatin MAT, Hart JP (1992) Analyst 117:1613

    Google Scholar 

  13. Oliveira Brett AM, Matysik FM (1997) Bioelectrochem Bioenerg 42:111

    CAS  Google Scholar 

  14. Zen JM, Chang MR, Ilangovan G (1999) Analyst 124:679

    Article  CAS  Google Scholar 

  15. Wang HS, Ju HX, Chen HY (2002) Anal Chim Acta 461:243

    Article  CAS  Google Scholar 

  16. Iijima S (1991) Nature 354:56

    CAS  Google Scholar 

  17. Dai H, Hafner JH, Rinzler AG, Colbert DT, Smalley RE (1996) Nature 384:147

    CAS  Google Scholar 

  18. Wong S, Joselevich E, Woolley A, Cheung C, Lieber C (1998) Nature 394:52

    Article  CAS  PubMed  Google Scholar 

  19. De Heer WA, Chatelain A, Ugarte D (1995) Science 270:1179

    Google Scholar 

  20. De Heer WA, Bonard JM, Fauth K (1997) Adv Mater 9:87

    Google Scholar 

  21. Baughman RH, Cui CC, Zakhidov AA, Iqbal Z, Barisci JN, Spinks GM, Wallace GG, Mazzoldi A, Rossi DD, Rinzler AG, Jaschinski O, Roth S, Kertesz M (1999) Science 284:1340

    CAS  PubMed  Google Scholar 

  22. Tans S, Verschueren A, Dekker C (1998) Nature 393:49

    Article  CAS  Google Scholar 

  23. Che GL, Lakschmi BB, Fisher ER, Martin CR (1998) Nature 393:346

    Article  Google Scholar 

  24. Dresselhaus MS (1992) Nature 358:195

    Google Scholar 

  25. Liu C, Fan YY, Liu M, Cong HT, Cheng HM, Dresselhaus MS (1999) Science 286:1127

    Article  CAS  PubMed  Google Scholar 

  26. Kong J, Franklin NR, Zhou CW, Chapline MG, Peng S, Cho K, Dai HJ (2000) Science 287:622

    Article  CAS  PubMed  Google Scholar 

  27. Britto PJ, Santhanam KSV, Ajayan PM (1996) Bioelectrochem Bioenerg 41:121

    CAS  Google Scholar 

  28. Davis JJ, Coles RJ, Hill HAO (1997) J Electroanal Chem 440:279

    CAS  Google Scholar 

  29. Luo HX, Shi ZJ, Li NQ, Gu ZN, Zhuang QK (2001) Anal Chem 73:915

    Article  CAS  PubMed  Google Scholar 

  30. Wu FH, Zhao GC, Wei XW (2002) Electrochem Commun 4:690

    Article  CAS  Google Scholar 

  31. Musamech M, Wang J, Merkoci A, Lin YH (2002) Electrochem Commun 4:743

    Article  Google Scholar 

  32. Sun YY, Fei JJ, Wu KB, Hu SS (2003) Anal Bioanal Chem 475:544

    Google Scholar 

  33. Tsang SC, Chen YK, Harris PJF, Green MLH (1994) Nature 372:159

    Google Scholar 

  34. Hiura H, Ebbesen TW, Tanigaki K (1995) Adv Mater 7:275

    CAS  Google Scholar 

  35. Garrett RH, Crishan CM (1995) Biochemistry. Saunders College Publishing, Orlando

  36. Paleček E (1983) In: Milazzo G (ed) Topics in bioelectrochemistry and bioenergetics. Wiley, London, p 65

  37. Davidson N (1972) The biochemistry of the nucleic acids, 7th edn. Cox and Nyman, Norfolk, UK, p 129

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Acknowledgements

The authors are grateful for financial support from the National Science Foundation of China (No.60171023).

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Authors and Affiliations

  1. Department of Chemistry, Wuhan University, 430072, Wuhan, P.R. China

    Kangbing Wu, Junjie Fei, Wen Bai & Shengshui Hu

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  1. Kangbing Wu
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  2. Junjie Fei
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  3. Wen Bai
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  4. Shengshui Hu
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Correspondence to Shengshui Hu.

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Wu, K., Fei, J., Bai, W. et al. Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode. Anal Bioanal Chem 376, 205–209 (2003). https://doi.org/10.1007/s00216-003-1887-0

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  • DOI: https://doi.org/10.1007/s00216-003-1887-0

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