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Use of reporter cell lines for detection of endocrine-disrupter activity

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Abstract

We have studied stable transformed human mammary cell lines with highly inducible steroid receptor-mediated luciferase reporter gene expression. Cells responding specifically to glucocorticoids, progestagens, androgens, or estrogens are described and characterized. The use of this high-throughput, cell-based assay for analysis of steroid (ant)agonists is reported. Systematic characterization of endocrine-disrupting activity on human receptors and in a human-cell system is interpreted for a selection of xenobiotics. We show that the phytoestrogens apigenin and genistin have progestagenic and androgenic activity, respectively. Finally, application of cell-based assays to the analysis of environmental samples is discussed.

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References

  1. Fry DM, Toone CK (1981) Science 213:922–924

    CAS  PubMed  Google Scholar 

  2. Colborn T, vom Saal FS, Soto AM (1993) Environ Health Perspect 101:378–384

    PubMed  Google Scholar 

  3. Rittler M, Castilla EE (2002) Cad Saude Publica 18:421–428

    PubMed  Google Scholar 

  4. Toppari J, Haavisto AM, Alanen M (2002) Cad Saude Publica 18:413–420

    PubMed  Google Scholar 

  5. Garcia-Rodriguez J, Garcia-Martin M, Nogueras-Ocana M, de Dios Luna-del-Castillo J, Espigares Garcia M, Olea N, Lardelli-Claret P (1996) Environ Health Perspect 104:1090–1095

    CAS  PubMed  Google Scholar 

  6. Pocar P, Brevini TA, Fischer B, Gandolfi F (2003) Reproduction 125:313–325

    CAS  PubMed  Google Scholar 

  7. Reijnders PJ (1986) Nature 324:456–457

    CAS  PubMed  Google Scholar 

  8. Birnbaum LS, Fenton SE (2003) Environ Health Perspect 111:389–394

    CAS  PubMed  Google Scholar 

  9. Garry VF, Harkins ME, Erickson LL, Long-Simpson LK, Holland SE, Burroughs BL (2002) Environ Health Perspect 110 Suppl 3:441–449

    Google Scholar 

  10. Nilsson S, Makela S, Treuter E, Tujague M, Thomsen J, Andersson G, Enmark E, Pettersson K, Warner M, Gustafsson JA (2001) Physiol Rev 81:1535–1565

    CAS  PubMed  Google Scholar 

  11. Evans RM (1988) Science 240:889–895

    CAS  PubMed  Google Scholar 

  12. Truss M, Beato M (1993) Endocr Rev 14:459–479

    CAS  PubMed  Google Scholar 

  13. Beato M (1989) Cell 56:335–344

    CAS  PubMed  Google Scholar 

  14. Muller M, Renkawitz R (1991) Biochim Biophys Acta 1088:171–182

    Article  CAS  PubMed  Google Scholar 

  15. Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM (1985) Nature 318:635–641

    CAS  PubMed  Google Scholar 

  16. Brinkmann AO, Faber PW, van Rooij HC, Kuiper GG, Ris C, Klaassen P, van der Korput JA, Voorhorst MM, van Laar JH, Mulder E, et al. (1989) J Steroid Biochem 34:307–310

    Article  CAS  PubMed  Google Scholar 

  17. Klein-Hitpass L, Ryffel GU, Heitlinger E, Cato AC (1988) Nucleic Acids Res 16:647–663

    CAS  PubMed  Google Scholar 

  18. Nordeen SK (1988) Biotechniques 6:454–458

    CAS  PubMed  Google Scholar 

  19. Mielke C, Kohwi Y, Kohwi-Shigematsu T, Bode J (1990) Biochemistry 29:7475–7485

    CAS  PubMed  Google Scholar 

  20. Molina A, Carpeaux R, Martial JA, Muller M (2002) Toxicol In Vitro 16:201–207

    Article  CAS  PubMed  Google Scholar 

  21. Payvar F, DeFranco D, Firestone GL, Edgar B, Wrange O, Okret S, Gustafsson JA, Yamamoto KR (1983) Cell 35:381–392

    CAS  PubMed  Google Scholar 

  22. Cato AC, Miksicek R, Schutz G, Arnemann J, Beato M (1986) Embo J 5:2237–2240

    CAS  PubMed  Google Scholar 

  23. Scheidereit C, Geisse S, Westphal HM, Beato M (1983) Nature 304:749–752

    CAS  PubMed  Google Scholar 

  24. Darbre P, Page M, King RJ (1986) Mol Cell Biol 6:2847–2854

    CAS  PubMed  Google Scholar 

  25. Cato AC, Henderson D, Ponta H (1987) Embo J 6:363–368

    CAS  PubMed  Google Scholar 

  26. Willemsen P, Scippo ML, Maghuin-Rogister G, Martial JA, Muller M (2002) Anal Chim Acta 473:119–126

    Article  CAS  Google Scholar 

  27. Jaffuel D, Roumestan C, Balaguer P, Henriquet C, Gougat C, Bousquet J, Demoly P, Mathieu M (2001) Steroids 66:597–604

    Article  CAS  PubMed  Google Scholar 

  28. Horwitz KB, Zava DT, Thilagar AK, Jensen EM, McGuire WL (1978) Cancer Res 38:2434–2437

    CAS  PubMed  Google Scholar 

  29. Blankvoort BM, de Groene EM, van Meeteren-Kreikamp AP, Witkamp RF, Rodenburg RJ, Aarts JM (2001) Anal Biochem 298:93–102

    Article  CAS  PubMed  Google Scholar 

  30. Walter P, Green S, Greene G, Krust A, Bornert JM, Jeltsch JM, Staub A, Jensen E, Scrace G, Waterfield M, et al. (1985) Proc Natl Acad Sci USA 82:7889–7893

    CAS  PubMed  Google Scholar 

  31. Green S, Walter P, Greene G, Krust A, Goffin C, Jensen E, Scrace G, Waterfield M, Chambon P (1986) J Steroid Biochem 24:77–83

    Article  CAS  PubMed  Google Scholar 

  32. Rosenberg RS, Grass L, Jenkins DJ, Kendall CW, Diamandis EP (1998) Biochem Biophys Res Commun 248:935–939

    Article  CAS  PubMed  Google Scholar 

  33. Scippo ML, Baise E, Francois JM, Corbaye T, Duyckaerts F, Rentier-Delrue F, Muller M, Maghuin-Rogister G, Martial JA (2000) Residues of veterinary drugs in foods, Proc Euroresidue IV Conf

  34. Yamasaki K, Sawaki M, Takatsuki M (2000) Environ Health Perspect 108:1147–1150

    PubMed  Google Scholar 

  35. Yamada T, Kunimatsu T, Sako H, Yabushita S, Sukata T, Okuno Y, Matsuo M (2000) Toxicol Sci 53:289–296

    Article  CAS  PubMed  Google Scholar 

  36. Fort DJ, Rogers RL, Paul RR, Miller MF, Clark P, Stover EL, Yoshioko J, Quimby F, Sower SA, Reed KL, Babbitt KJ, Rolland R (2001) J Appl Toxicol 21:199–209

    Article  CAS  PubMed  Google Scholar 

  37. Laidley CW, Thomas P (1997) Biol Reprod 56:931–937

    CAS  PubMed  Google Scholar 

  38. EDSTAC (1998) US Environmental Protection Agency: Endocrine Disrupter Screening and Testing Advisory Committee (EDSTAC), Final report

    Google Scholar 

  39. OECD (2001) Organisation for Economic Co-operation and Development: Appraisal of test methods for sex hormone disrupting chemicals

  40. Jobling S (1998) Pure Appl Chem 70:1805–1827

    CAS  Google Scholar 

  41. Zacharewski T (1998) Environ Health Perspect 106:577–582

    PubMed  Google Scholar 

  42. Fang H, Tong W, Perkins R, Soto AM, Prechtl NV, Sheehan DM (2000) Environ Health Perspect 108:723–729

    CAS  PubMed  Google Scholar 

  43. Scippo ML, Van de Weerdt C, Willemsen P, Francois JM, Rentier-Delrue F, Muller M, Martial JA, Maghuin-Rogister G (2002) Anal Chim Acta 473:135–141

    Article  CAS  Google Scholar 

  44. Legler J, van den Brink CE, Brouwer A, Murk AJ, van der Saag PT, Vethaak AD, van der Burg B (1999) Toxicol Sci 48:55–66

    Article  CAS  PubMed  Google Scholar 

  45. Dodge JA, Glasebrook AL, Magee DE, Phillips DL, Sato M, Short LL, Bryant HU (1996) J Steroid Biochem Mol Biol 59:155–161

    Article  CAS  PubMed  Google Scholar 

  46. Purvis IJ, Chotai D, Dykes CW, Lubahn DB, French FS, Wilson EM, Hobden AN (1991) Gene 106:35–42

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was financially supported by the Direction Générale des Technologies, de la Recherche et de l'Energie du Ministère de la Région Wallonne, by the Ministère des Classes Moyennes et de l'Agriculture (DG6). We also would like to thank R.M. Evans for kindly providing the RS-hGRα expression vector, P. Chambon for the PR expression vector, A. Brinkmann for the AR vector, G. Ryffel for Vit-gpt, and R. Carpeaux, S. Orban, C. Namurois, and T. Corbaye for excellent technical assistance.

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de Liège, Bâtiment de Chimie B-6, Sart Tilman, 4000, Liège, Belgium

    Philippe Willemsen, Joseph A. Martial & Marc Muller

  2. Département des Sciences des Denrées Alimentaires, Université de Liège, Bâtiment B-43bis, Sart Tilman, 4000, Liège, Belgium

    Marie-Louise Scippo & Guy Maghuin-Rogister

  3. Instituto de Bioquimica, Universidad Austral de Chile, Valdivia, Chile

    Gudrun Kausel & Jaime Figueroa

Authors
  1. Philippe Willemsen
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  2. Marie-Louise Scippo
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  3. Gudrun Kausel
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  4. Jaime Figueroa
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  5. Guy Maghuin-Rogister
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  6. Joseph A. Martial
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  7. Marc Muller
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Corresponding author

Correspondence to Marc Muller.

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Willemsen, P., Scippo, ML., Kausel, G. et al. Use of reporter cell lines for detection of endocrine-disrupter activity. Anal Bioanal Chem 378, 655–663 (2004). https://doi.org/10.1007/s00216-003-2217-2

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  • DOI: https://doi.org/10.1007/s00216-003-2217-2

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