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Integrated microfluidic device with an electroplated palladium decoupler for more sensitive amperometric detection of the 8-hydroxy-deoxyguanosine (8-OH-dG) DNA adduct

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Abstract

8-Hydroxy-deoxyguanosine (8-OH-dG) DNA adduct is one of the most frequently used biomarkers reporting on the oxidative stress that leads to DNA damage. More sensitive and reliable microfluidic devices are needed for the detection of these biomarkers of interest. We have developed a capillary electrophoresis (CE)-based microfluidic device with an electroplated palladium decoupler that provides significantly improved detection limit, separation efficiency, and resolving power. The poly(dimethylsiloxane) (PDMS)/glass hybrid device has fully integrated gold microelectrodes covered in situ with palladium nanoparticles using an electroplating technique. The performance and coverage of the electrodes electroplated with palladium particles were evaluated electrochemically and via scanning electron microscope (SEM) imaging, respectively. The performance of the device was tested and evaluated with different buffer systems, pH values, and electric field strengths. The results showed that this device has significantly improved resolving power, even at separation electric field strengths as high as 600 V cm−1. The detection limit for the 8-OH-dG adduct is about 20 attomoles; the concentration limit is on the order of 100 nM (S/N = 3). A linear response is reported for both 8-OH-dG and dG in the range from 100 nM to 150 μM (≈100 pA μM−1) with separation efficiencies of approximately 120,000–170,000 plates m−1.

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Abbreviations

AD:

amperometric detection

CE:

capillary electrophoresis

dG:

deoxyguanosine

8-OH-dG:

8-hydroxy-deoxyguanosine

ED:

electrochemical detection

EG:

electrophoretic ground

LOD:

limit of detection

PDMS:

poly(dimethylsiloxane)

WE:

working electrode

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Acknowledgement

This work was supported by a grant from the National Cancer Institute (ProgramProject Grant 2PO1 CA49210-12) and in part by the NIH COBRE award 1 P20 R15563, and matching support from the State of Kansas.

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Author notes

  1. Abdulilah A. Dawoud

    Present address: Virginia Bioinformatics Institute, Virginia Tech., Blacksburg, VA, 24061, USA

Authors and Affiliations

  1. Department of Chemistry, Iowa State University, Ames, IA, 50011, USA

    Abdulilah A. Dawoud & Toshikazo Kawaguchi

  2. Department of Chemistry, Kansas State University, Manhattan, KS, 66502, USA

    Ryszard Jankowiak

Authors
  1. Abdulilah A. Dawoud
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  2. Toshikazo Kawaguchi
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  3. Ryszard Jankowiak
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Corresponding authors

Correspondence to Abdulilah A. Dawoud or Ryszard Jankowiak.

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Dawoud, A.A., Kawaguchi, T. & Jankowiak, R. Integrated microfluidic device with an electroplated palladium decoupler for more sensitive amperometric detection of the 8-hydroxy-deoxyguanosine (8-OH-dG) DNA adduct. Anal Bioanal Chem 388, 245–252 (2007). https://doi.org/10.1007/s00216-007-1203-5

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  • DOI: https://doi.org/10.1007/s00216-007-1203-5

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