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Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk

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Abstract

A novel rapid (20 min) fluorescent lateral flow test for chloramphenicol (CAP) detection in milk was developed. The chosen format is a binding-inhibition assay. Water-soluble quantum dots with an emission peak at 625 nm were applied as a label. Milk samples were diluted by 20 % with phosphate buffer to eliminate the matrix effect. The result of the assay could be seen by eye under UV light excitation or registered by a portable power-dependent photometer. The limit of CAP detection by the second approach is 0.2 ng/mL, and the limit of quantitation is 0.3 ng/mL.

Principle of proposed immunoassay of chloramphenicol using quantum dots

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References

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Acknowledgments

This work was supported by the Federal Target Program “Research and development on priority directions of scientific-technological complex of Russia in 2007–2013” (State Contract No 16.512.11.2125). We are grateful to P.G. Sveshnikov and O.N. Solopova (All-Russian Research Center of Molecular Diagnostics and Therapy, Moscow, Russia) for the preparation and assignment of anti-CAP antibodies. We are also grateful to E.A. Zvereva (Institute of Biochemistry, Moscow, Russia) for the synthesis of the CAP–protein conjugates.

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Authors and Affiliations

  1. A.N. Baсh Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia

    Anna N. Berlina, Nadezhda A. Taranova, Anatoly V. Zherdev, Yuri Y. Vengerov & Boris B. Dzantiev

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  1. Anna N. Berlina
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  2. Nadezhda A. Taranova
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  3. Anatoly V. Zherdev
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  4. Yuri Y. Vengerov
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  5. Boris B. Dzantiev
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Correspondence to Anna N. Berlina.

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Berlina, A.N., Taranova, N.A., Zherdev, A.V. et al. Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk. Anal Bioanal Chem 405, 4997–5000 (2013). https://doi.org/10.1007/s00216-013-6876-3

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  • DOI: https://doi.org/10.1007/s00216-013-6876-3

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