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Isolation and characterization of protein fractions from deoiled rice bran

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Abstract

Rice bran contains underutilised protein materials. Sequential extraction of rice bran protein (RBP) from defatted rice bran was conducted based on the differences in their solubility. Three extraction methods were investigated. Method 1 involved the isoelectric and acetone precipitation using water, 50 g kg−1 NaCl, 0.02 mol L−1 NaOH and 70% ethanol as extracting solvents for albumin (pH 4.1), globulin (pH 4.3), glutelin (pH 4.8) and prolamin, respectively. Method 2 adopted dialysis and sequential extraction was carried out with 20 g kg−1 NaCl, 70% ethanol, 0.1 mol L−1 acetic acid and 0.1 mol L−1 NaOH solution as extracting solvents. Method 3 combined dialysis, isoelectric and acetone precipitation for the extraction. Based on the yields and data obtained from sodium dodecyl sulphate polyacrylamide gel electrophoresis, size-exclusion chromatography and differential scanning calorimetry, method 3 was chosen for the isolation and characterization of RBPs. Rice bran protein fraction (RBPF)—albumin, globulin, glutelin and prolamin were obtained in good yields. Denaturation temperature and enthalpy values of denaturation of RBPF vary. Highest phytate content was found in albumin and lowest in prolamin. The highest antioxidative and hemagglutinating activities were observed in albumin.

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Acknowledgments

This work was supported in part by funds from the Ministry of Agriculture, Forestry and Fisheries of the Japanese Government. A.P.A. also gratefully acknowledges the Monbugakakusho (Japanese Ministry of Education, Culture, Science and Technology) for granting him a scholarship to undertake studies in Japan.

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Authors and Affiliations

  1. Department of Food Science and Engineering, Ladoke Akintola University of Technology, PMB 4000, Ogbomosho, Oyo State, Nigeria

    Abayomi P. Adebiyi

  2. Department of Pure and Applied Biology, Ladoke Akintola University of Technology, PMB 4000, Ogbomosho, Oyo State, Nigeria

    Ayobamitale O. Adebiyi

  3. Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, 981-8555, Japan

    Yusuke Hasegawa, Tomohisa Ogawa & Koji Muramoto

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  1. Abayomi P. Adebiyi
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  2. Ayobamitale O. Adebiyi
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  3. Yusuke Hasegawa
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  4. Tomohisa Ogawa
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  5. Koji Muramoto
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Correspondence to Abayomi P. Adebiyi.

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Adebiyi, A.P., Adebiyi, A.O., Hasegawa, Y. et al. Isolation and characterization of protein fractions from deoiled rice bran. Eur Food Res Technol 228, 391–401 (2009). https://doi.org/10.1007/s00217-008-0945-4

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  • DOI: https://doi.org/10.1007/s00217-008-0945-4

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