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Endocytosis of Tartrate-Resistant Acid Phosphatase by Osteoblast-Like Cells Is Followed by Inactivation of the Enzyme

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Abstract

Tartrate-resistant acid phosphatase (TRACP) is generally used as a marker of osteoclasts. Yet, other bone-associated cells, such as osteoblasts and osteocytes, may also express activity of this enzyme. Osteoblasts containing TRACP activity are seen particularly in the vicinity of areas of bone resorption, suggesting that osteoclasts somehow induce TRACP activity in osteoblasts. In a recent study, we found that osteoblast-like cells appeared to have the capacity to endocytose TRACP released by osteoclast precursors. In the present study, we investigated the endocytosis of TRACP in more detail as well as the fate of the endocytosed enzyme. We found that incubation of osteoblast-like cells with TRACP-coated beads resulted in attachment of a high number of beads to the cells. After culturing osteoblast-like cells with medium conditioned by blood monocytes that contain TRACP, activity of the enzyme was found in the cells. Following replacement of the medium by normal medium that did not contain TRACP, a decrease in the level of TRACP activity in osteoblast-like cells occurred. Our data strongly suggest that osteoblast-like cells recognize TRACP released by osteoclast precursors and that upon endocytosis inactivation of the enzyme occurs. We propose that uptake of the enzyme is important for the control of enzyme activity, thereby preventing degradation of matrix constituents.

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Authors and Affiliations

  1. Experimental Periodontology, Department of Periodontology, Academic Center for Dentistry Amsterdam, Universiteit van Amsterdam and Vrije Universiteit, Louwesweg 1, Amsterdam, EA, 1066, The Netherlands

    S. Perez-Amodio, D. C. Jansen & W. Beertsen

  2. Department of Cell Biology and Histology, Academic Medical Center, Universiteit van Amsterdam, P.O. Box 22700, Amsterdam, DE, 1100, The Netherlands

    W. Tigchelaar-Gutter

  3. Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam, Universiteit van Amsterdam and Vrije Universiteit, van der Boechorststraat 7, Amsterdam, BT, 1081, The Netherlands

    V. Everts

Authors
  1. S. Perez-Amodio
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  2. D. C. Jansen
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  3. W. Tigchelaar-Gutter
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  4. W. Beertsen
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  5. V. Everts
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Correspondence to V. Everts.

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Perez-Amodio, S., Jansen, D.C., Tigchelaar-Gutter, W. et al. Endocytosis of Tartrate-Resistant Acid Phosphatase by Osteoblast-Like Cells Is Followed by Inactivation of the Enzyme. Calcif Tissue Int 78, 248–254 (2006). https://doi.org/10.1007/s00223-005-0185-6

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  • DOI: https://doi.org/10.1007/s00223-005-0185-6

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