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Interaction Between Equally Charged Membrane Surfaces Mediated by Positively and Negatively Charged Macro-Ions

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Abstract

In biological systems, charged membrane surfaces are surrounded by charged molecules such as electrolyte ions and proteins. Our recent experiments in the systems of giant phospholipid vesicles indicated that some of the blood plasma proteins (macro-ions) may promote adhesion between equally charged membrane surfaces. In this work, theory was put forward to describe an IgG antibody-mediated attractive interaction between negatively charged membrane surfaces which was observed in experiments on giant phospholipid vesicles with cardiolipin-containing membranes. The attractive interactions between negatively charged membrane surfaces in the presence of negatively and positively charged spherical macro-ions are explained using functional density theory and Monte Carlo simulations. Both, the rigorous solution of the variational problem within the functional density theory and the Monte Carlo simulations show that spatial and orientational ordering of macro-ions may give rise to an attractive interaction between negatively charged membrane surfaces. It is also shown that the distinctive spatial distribution of the charge within the macro-ions (proteins) is essential in this process.

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Authors and Affiliations

  1. Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000, Ljubljana, Slovenia

    Šárka Perutková, Klemen Bohinc & Aleš Iglič

  2. Department of Rheumatology, University Medical Center Ljubljana, Vodnikova 62, 1000, Ljubljana, Slovenia

    Mojca Frank & Blaž Rozman

  3. Faculty of Health Studies, University of Ljubljana, Poljanska 26a, 1000, Ljubljana, Slovenia

    Klemen Bohinc

  4. Laboratory of Telecommunications, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000, Ljubljana, Slovenia

    Goran Bobojevič

  5. Laboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, 1000, Ljubljana, Slovenia

    Goran Bobojevič, Jasna Zelko & Veronika Kralj-Iglič

Authors
  1. Šárka Perutková
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  2. Mojca Frank
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  3. Klemen Bohinc
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  4. Goran Bobojevič
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  5. Jasna Zelko
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  6. Blaž Rozman
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  7. Veronika Kralj-Iglič
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  8. Aleš Iglič
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Correspondence to Aleš Iglič.

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Perutková, Š., Frank, M., Bohinc, K. et al. Interaction Between Equally Charged Membrane Surfaces Mediated by Positively and Negatively Charged Macro-Ions. J Membrane Biol 236, 43–53 (2010). https://doi.org/10.1007/s00232-010-9278-x

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  • DOI: https://doi.org/10.1007/s00232-010-9278-x

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