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Bacterial translocation in experimental uremia

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Abstract

The aim of this study was to investigate whether or not experimental uremia would induce bacterial translocation. Forty male Wistar rats were randomized into two groups: uremic (n=20) and control (n=20). Under anesthesia, the upper and lower left renal poles and the marginal lateral parenchyma were excised in uremic group. Seven days later, in a second operation the whole right kidney was removed. In control animals, two sham operations with the same interval were performed. After 60 days from the first operation, the liver, spleen and the mesenteric lymph nodes (MLN) were excised and cultured. Blood samples were sent for biochemical analysis (BUN, creatinine, sodium and potassium) and cultured. Specimens of the jejunum (1 cm below the Treitz angle) and ileum (1 cm above the ileocecal valve) were collected and sent for histological examination and scored for the degree of inflammation of the mucosa using a classification proposed by Chiu et al. in 1970. Uremic rats presented higher BUN, creatinine and potassium than controls. Bacterial translocation was more frequent in uremic than in control animals (8/20 (40%) vs. 1/20 (5%); p=0.02). Translocation in uremic rats was observed mainly at the MLN (all eight cases). Both at the jejunum (uremic=3 [0–5] vs. control=2 [0–4]; p=0.04) and the ileum (uremic=2 [0–5] vs. control=0 [0–3]; p=0.01), inflammation score was higher in uremic rats than in controls. The intestinal mucosa barrier is impaired and bacterial translocation occurs in experimental uremia.

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Authors and Affiliations

  1. Department of Surgery, Medical School of Medicine and Collective Health Institute, Federal University of Mato Grosso, Rua Estevão de Mendonça 81 apto 801, 78045-200, Cuiaba, MT, Brazil

    José Eduardo de Aguilar-Nascimento

  2. Department of Surgery, Medical School of Medicine and Collective Health Institute, Federal University of Mato Grosso, Cuiaba, MT, Brazil

    João Bosco de Almeida Duarte, José Eduardo de Aguilar-Nascimento, Mariana Nascimento & Rubens Jardim Nochi Jr.

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  1. João Bosco de Almeida Duarte
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  2. José Eduardo de Aguilar-Nascimento
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  3. Mariana Nascimento
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  4. Rubens Jardim Nochi Jr.
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Correspondence to José Eduardo de Aguilar-Nascimento.

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de Almeida Duarte, J.B., de Aguilar-Nascimento, J.E., Nascimento, M. et al. Bacterial translocation in experimental uremia. Urol Res 32, 266–270 (2004). https://doi.org/10.1007/s00240-003-0381-7

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