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Ecotoxicological Effect of Sublethal Exposure to Zinc Oxide Nanoparticles on Freshwater Snail Biomphalaria alexandrina

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Abstract

Freshwater snails are used as sensitive biomarkers of aquatic ecosystem pollution. The potential impacts of zinc oxide nanoparticles (ZnONPs) on aquatic ecosystems have attracted special attention due to their unique properties. The present investigation was designed to evaluate the possible mechanisms of ecotoxicological effects of ZnONPs on freshwater snail Biomphalaria alexandrina. ZnONPs showed molluscicidal activity against B. alexandrina snails, and the LC50 was 145 μg/ml. Two tested concentrations of ZnONPs were selected: The first concentration was equivalent to LC10 (7 μg/ml), and the second was equivalent to LC25 (35 μg/ml). Exposure to ZnONPs (7 and 35 μg/ml) for three consecutive weeks significantly induced malondialdehyde and nitric oxide with concomitant decreases in glutathione and glutathione-S-transferase levels in hemolymph and soft tissues of treated snails. Moreover, ZnONPs elicited a significant decrease in total protein and albumin contents coinciding with enhancement of total lipids and cholesterol levels as well as activities of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in hemolymph and soft tissues of treated snails. This study highlights the potential ecological implications of ZnONP release in aquatic environments and may serve to encourage regulatory agencies in Egypt to more carefully monitor and regulate the industrial use and disposal of ZnONPs.

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Authors and Affiliations

  1. Zoology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt

    Sohair R. Fahmy, Fathy Abdel-Ghaffar & Dawlat A. Sayed

  2. Medical Malacology Department, Theodor Bilharz Research Institute, Giza, Egypt

    Fayez A. Bakry

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  1. Sohair R. Fahmy
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  2. Fathy Abdel-Ghaffar
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  3. Fayez A. Bakry
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Fahmy, S.R., Abdel-Ghaffar, F., Bakry, F.A. et al. Ecotoxicological Effect of Sublethal Exposure to Zinc Oxide Nanoparticles on Freshwater Snail Biomphalaria alexandrina . Arch Environ Contam Toxicol 67, 192–202 (2014). https://doi.org/10.1007/s00244-014-0020-z

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