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Interleukin–10 promoter haplotypes are differently distributed in the Brazilian versus the Dutch population

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Abstract.

The frequency of five different single nucleotide polymorphisms of the promoter interleukin-10 (IL–10) gene (–3575, –2849, 2763, –1082, –819) was compared between two healthy populations, one originating from the Netherlands and one from Rio de Janeiro, Brazil. A total of 321 Caucasian Dutch individuals and 293 Brazilians, grouped as Afro–Brazilians and Euro–Brazilians, were genotyped using PCR–RFLP. The frequencies of the genotypes in the Brazilian population were different (P<0.05) from the frequencies in the Dutch population in all but one (–2763) genotype. The comparison of genotype frequencies between Afro– and Euro–Brazilians did not demonstrate any differences. The haplotype combination of the most-distant three polymorphisms showed strong linkage disequilibrium. All eight possible combinations were observed in Brazilians, but only seven in Dutch Caucasians. The haplotype frequencies were also significantly different between Brazilians when compared with Dutch and also between Euro–Brazilians and Dutch. No differences were observed in haplotype frequencies between Afro–Brazilians and Euro–Brazilians. The −3575T/–2849G/–2763C is more frequent, while the AAA haplotype was much less represented in the Brazilian than in the Dutch population. The haplotype TAC, which was described in African–Americans, was observed only in Brazilians, almost exclusively among those of European origin. The results corroborate the data indicating that the Brazilian population exhibits a genetic admixture of Africans, Europeans, and Amerindians, and the data may serve as a background for clinical and immunological studies involving the IL-10 locus.

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References

  • Alves–Silva J, Santos MS, Guimarães PEM, Ferreira ACS, Bandelt H–J, Pena SDJ, Prado VF (2000) The ancestry of Brazilian mtDNA lineages. Am J Hum Genet 67:444–461

    CAS  PubMed  Google Scholar 

  • Carvalho–Silva DR, Santos FR, Rocha J, Pena SD (2001) The phylogeography of Brazilian Y–chromosome lineages. Am J Hum Genet 68:281–286

    Article  CAS  PubMed  Google Scholar 

  • Cox ED, Hoffmann SC, DiMercurio BS, Wesley RA, Harlan DM, Kirk AD, Blair PJ (2001) Cytokine polymorphic analyses indicate ethnic differences in the allelic distribution of interleukin–2 and interleukin–6 72:720–726

  • De Vries RR, Meera KP, Bernini LF, Loghem E van, Rood JJ van (1979) Genetic control of survival in epidemics. J Immunogenet 6:271–287

    PubMed  Google Scholar 

  • Eskdale J, Mc Nicholl J, Wordsworth P, Jonas P, Huizinga T, Gallagher G (1998) Interleukin–10 microsatellite polymorphism and IL–10 locus alleles in rheumatoid arthritis susceptibility Lancet 352:1282–1283

    Google Scholar 

  • Eskdale J, Keijsers V, Huizinga T, Gallagher G (1999) Microsatellite alleles and single nucleotide polymorphism (SNP) combine to form four major haplotype families at the human interleukin–10 (IL–10) locus. Genes Immun 1:151–155

    Article  CAS  PubMed  Google Scholar 

  • Gerard C, Bruyns C, Marchant A, Abramowicz D, Vandenabeele P, Delvaux A, Fiers W, Goldman M, Velu T (1993) Interleukin 10 reduces the release of tumor necrosis factor and prevents lethality in experimental endotoxemia. J Exp Med 177:547–550

    CAS  PubMed  Google Scholar 

  • Gibson AW, Edberg JC, Wu J, Westerdorp RGJ, Huizinga TWJ, Kimberly RP (2001) Novel single nucleotide polymorphisms in the distal IL–10 promoter affect IL–10 production and enhance the risk of systemic lupus erythematosus. J Immunol 166:3915–3922

    CAS  PubMed  Google Scholar 

  • Llorente L, Zou W, Levy Y, Richaud–Patin Y, Wijdenes J, Alcocer–Varela J, Morel–Fourrier B, Brouet JC, Alarcon–Segovia D, Galanaud P (1995) Role of interleukin–10 in the lymphocyte hyperactivity and autoantibody production in human systemic lupus erythematosus. J Exp Med 1:839–844

    Google Scholar 

  • Meenagh A, Williams F, Ross AO, Patterson C, Gorodezky C, Hammond M, Leheny WA, Midlleton D (2002) Frequency of cytokine polymorphisms in population from Western Europe, Africa, Asia, Middle East and South America. Human Immunol 63:1055–1061

    Article  CAS  Google Scholar 

  • Mok CC, Lanchbury JS, Chan DW, Lau CS (1998) IL–10 promoter polymorphism in southern Chinese patients with systemic lupus erythematosus. Arthritis Rheum 41:1090–1097

    Article  CAS  PubMed  Google Scholar 

  • Moraes ME, Fernandez–Vina M, Salatiel I, Tsai S, Moraes JR, Stastny P (1993) HLA class II DNA typing in two Brazilian population. Tissue Antigens 41:238–242

    CAS  PubMed  Google Scholar 

  • Moraes MO, Sarno EN, Teles RMB, Saraiva BCC, Nery JAC, Sampaio EP (2000) Anti–inflammatory drugs block cytokine mRNA accumulation in the skin and improve the clinical condition of reactional leprosy patients. J Invest Dermatol 115:935–941

    CAS  PubMed  Google Scholar 

  • Roy S, McGuire W, Mascie–Taylor CG, Saha B, Hazra SK, Hill AV, Kwiatkowski D (1997) Tumor necrosis factor promoter polymorphism and susceptibility to lepromatous leprosy. J Infect Dis 176:530–532

    CAS  PubMed  Google Scholar 

  • Santos AR, Almeida AS, Suffys PN, Moraes MO, Filho VF, Mattos HJ, Nery JAC, Cabello PH, Sampaio EP, Sarno EN (2000) Tumor necrosis factor promoter polymorphism (TNF2) seems to protect against development of severe forms of leprosy in pilot study in Brazilian patients Int J Lepr 68:325–327

    Google Scholar 

  • Santos AR, Suffys PN, Vanderborght PR, Moraes MO, Vieira LM, Cabello PH, Bakker AM, Matos HJ, Huizinga TW, Ottenhoff TH, Sampaio EP, Sarno EN (2002) Role of tumor necrosis factor–alpha and interleukin–10 promoter gene polymorphisms in leprosy. J Infect Dis 186:1687–1691

    Article  CAS  PubMed  Google Scholar 

  • Shaw MA, Donaldson IJ, Collins A, Peacock CS, Lins–Lainson Z, Shaw JJ, Ramos F, Silveira F, Blackwell JM (2001) Association and linkage of leprosy phenotypes with HLA class II and tumor necrosis factor genes. Genes Immun 2:196–204

    Article  CAS  PubMed  Google Scholar 

  • Terwilliger JD, Ott J (1994) Linkage disequilibrium between alleles at marker loci 188–193. In: Handbook of human genetics linkage. The John Hopkins University Press, Baltimore

  • Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV (1997) An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 24:1-8

    CAS  Google Scholar 

  • Volk H, Asadullah K, Gallagher G, Sabat R, Grutz G (2001) IL–10 and its homologs: important immune mediators and emerging immunotherapeutic targets. Trends Immunol 22:414–417

    Article  CAS  PubMed  Google Scholar 

  • Westerdorp RGJ, Dunne FM van, Kirkwood TBL, Helmerhost FM, Huizinga TWJ (2001) Optimizing human fertility and survival. Nat Med 7:873

    Article  PubMed  Google Scholar 

  • Williams F, Meenagh A, Darke C, Acosta A, Daar AS, Gorodezky C, Hammond M, Nascimento E, Middleton D (2001) Analysis of the distribution of HLA–B alleles in the population from five continents Hum Immunol 62:645–650

    Google Scholar 

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Acknowledgements.

We are grateful to Valcemir F. Silva–Filho for technical assistance.

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Authors and Affiliations

  1. Leprosy Laboratory, Tropical Medicine Department, Oswaldo Cruz Institute, Fiocruz, Av. Brasil, 4365 Manguinhos, RJ 21045–900, Rio de Janeiro, Brazil

    Milton O. Moraes, Adalberto R. Santos, Patrícia R. Vanderborght, Elizabeth P. Sampaio & Euzenir N. Sarno

  2. Laboratório de Imunogénetica, Instituto Nacional do Cancer, Ministério da Saúde, Rio de Janeiro, Brazil

    Maria E. Moraes & José R. Moraes

  3. Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands

    Tom H. M. Ottenhoff

  4. Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands

    Joris J. M. Schonkeren & Tom W. J. Huizinga

Authors
  1. Milton O. Moraes
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  2. Adalberto R. Santos
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  3. Joris J. M. Schonkeren
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  4. Patrícia R. Vanderborght
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  5. Tom H. M. Ottenhoff
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  6. Maria E. Moraes
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  7. José R. Moraes
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  8. Elizabeth P. Sampaio
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  9. Euzenir N. Sarno
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  10. Tom W. J. Huizinga
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Correspondence to Milton O. Moraes.

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Moraes, M.O., Santos, A.R., Schonkeren, J.J.M. et al. Interleukin–10 promoter haplotypes are differently distributed in the Brazilian versus the Dutch population. Immunogenetics 54, 896–899 (2003). https://doi.org/10.1007/s00251-003-0543-3

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  • DOI: https://doi.org/10.1007/s00251-003-0543-3

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