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Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor

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Abstract

The doubling time of anaerobic ammonium-oxidizing (anammox) bacteria in an anaerobic biological filtrated (ABF) reactor was determined. Fluorescence in situ hybridization analysis was used to detect and count anammox bacteria cells in anammox sludge. As a result, the populations of anammox bacteria at 14th and 21st days were 1.1×106 and 1.7×107 cells/ml reactor, respectively. From these results, the doubling time of anammox bacteria was calculated as 1.8 days, and the specific growth rate (μ) was 0.39 day−1. This result indicated that the anammox bacteria have higher growth rate than the reported value (doubling time, 11 days). Furthermore, it was clearly demonstrated that nitrogen conversion rate was proportional to the population of anammox bacteria. Maintaining the ideal environment for the growth of anammox bacteria in the ABF reactor might lead to faster growth. This is the first report of the growth rate of anammox bacteria based on the direct counting of anammox bacteria.

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Authors and Affiliations

  1. Hitachi Plant Engineering and Construction Co., Ltd., Kami-Hongo 537, Matsudo, Chiba, 271-0064, Japan

    Kazuichi Isaka & Tatsuo Sumino

  2. Department of Chemical Engineering, Waseda University, Ohkubo 3-4-1, Shinjuku-ku, Tokyo, 169-8555, Japan

    Yasuhiro Date, Sachiko Yoshie & Satoshi Tsuneda

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  1. Kazuichi Isaka
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  2. Yasuhiro Date
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  3. Tatsuo Sumino
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  4. Sachiko Yoshie
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  5. Satoshi Tsuneda
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Correspondence to Kazuichi Isaka.

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Isaka, K., Date, Y., Sumino, T. et al. Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor. Appl Microbiol Biotechnol 70, 47–52 (2006). https://doi.org/10.1007/s00253-005-0046-2

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  • DOI: https://doi.org/10.1007/s00253-005-0046-2

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