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Synergy between xylanases from glycoside hydrolase family 10 and family 11 and a feruloyl esterase in the release of phenolic acids from cereal arabinoxylan

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Abstract

The bioconversion of waste residues (by-products) from cereal processing industries requires the cooperation of enzymes able to degrade xylanolytic and cellulosic material. The type A feruloyl esterase from Aspergillus niger, AnFaeA, works synergistically with (1→4)-β-d-xylopyranosidases (xylanases) to release monomeric and dimeric ferulic acid (FA) from cereal cell wall-derived material. The esterase was more effective with a family 11 xylanase from Trichoderma viride in releasing FA and with a family 10 xylanase from Thermoascus aurantiacus in releasing the 5,5′ form of diferulic acid from arabinoxylan (AX) derived from brewers’ spent grain. The converse was found for the release of the phenolic acids from wheat bran-derived AXs. This may be indicative of compositional differences in AXs in cereals.

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Abbreviations

AX:

Arabinoxylan

BSG:

Brewers’ spent grain

FAE:

Feruloyl esterase

GHIO:

Family 10 xylanase

GH11:

Family 11 xylanase

WB:

wheat bran

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Acknowledgements

This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC), UK; the General Secretariat for Research and Technology (GSRT), Greece; the British Council (Athens); the European Social Fund; and the Department of the Environment, Food and Rural Affairs (DEFRA), UK. We would like to express our gratitude to Scottish Courage Ltd for supplying BSG and to ARD for the WB sample.

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Authors and Affiliations

  1. Sustainability of the Food Chain Exploitation Platform, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK

    C. B. Faulds, G. Mandalari & K. W. Waldron

  2. Department of Organic and Biological Chemistry, University of Messina, Sicily, Italy

    G. Mandalari & R. B. Lo Curto

  3. Department of Pharma-Biology, University of Messina, Sicily, Italy

    G. Mandalari & G. Bisignano

  4. Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700, Athens, Greece

    P. Christakopoulos

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  1. C. B. Faulds
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  2. G. Mandalari
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  3. R. B. Lo Curto
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  4. G. Bisignano
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  5. P. Christakopoulos
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  6. K. W. Waldron
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Correspondence to C. B. Faulds.

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Faulds, C.B., Mandalari, G., Lo Curto, R.B. et al. Synergy between xylanases from glycoside hydrolase family 10 and family 11 and a feruloyl esterase in the release of phenolic acids from cereal arabinoxylan. Appl Microbiol Biotechnol 71, 622–629 (2006). https://doi.org/10.1007/s00253-005-0184-6

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  • DOI: https://doi.org/10.1007/s00253-005-0184-6

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