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Growth promoting effect of a transgenic Bacillus mucilaginosus on tobacco planting

  • Applied Microbial and Cell Physiology
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Abstract

In this study, we have investigated the plant growth promoting effect of Bacillus mucilaginosus strain D4B1, a rhizosphere soil organism, and its transgenic strain NKTS-3 on tobacco planting. The transgenic strain contains a phytase expression cassette that can express high active phytase extracellularly and hydrolyze phytate in the soil to liberate inorganic phosphorus for the growth of tobacco plants. Greenhouse study and field experiments showed that both wild-type B. mucilaginosus and the transgenic strain could promote tobacco plant growth. Moreover, the transgenic strain promoted tobacco plant growth (235% more than control in pot experiments and 125% more than control in field experiments) was higher than the wild-type B. mucilaginosus (183% more than control in pot experiments and 108% more than control in field experiments). In addition, the inoculation with transgenic rhizobacteria could significantly improve root acquisition of phosphorus and increase the phosphorus content of the plant.

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Acknowledgment

We thank the Institute of Soil and fertilizer, Shanxi Province Academy of Agricultural Sciences for providing field experiment site and Prof. Zhang Keqin for kindly providing strain B. mucilaginosus. We are grateful to Prof. Yaoting Yu for critical review of this manuscript.

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Authors and Affiliations

  1. The Key Laboratory of Bioactive Material, Ministry of Education, China, Life Science College, Nankai University, Tianjin, 300071, China

    Xin Li, Zhiqiang Wu, Weidong Li, Ruixiang Yan & Minggang Li

  2. Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences, No. 30 Baishi QialLu, HaiDian District, Beijing, 100081, China

    Li Li & Jun Li

  3. Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, 36849, USA

    Yihang Li

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  1. Xin Li
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  2. Zhiqiang Wu
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  3. Weidong Li
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  4. Ruixiang Yan
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  5. Li Li
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  6. Jun Li
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  7. Yihang Li
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  8. Minggang Li
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Correspondence to Minggang Li.

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Li, X., Wu, Z., Li, W. et al. Growth promoting effect of a transgenic Bacillus mucilaginosus on tobacco planting. Appl Microbiol Biotechnol 74, 1120–1125 (2007). https://doi.org/10.1007/s00253-006-0750-6

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  • DOI: https://doi.org/10.1007/s00253-006-0750-6

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