Skip to main content
Log in

Transcriptional regulation of two cellobiohydrolase encoding genes (cel1 and cel2) from the wood-degrading basidiomycete Polyporus arcularius

  • Applied Genetics and Molecular Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

In the current studies, we sequenced and characterized the genomic and complementary deoxyribonucleic acid clones encoding the cellobiohydrolase encoding genes cel1 and cel2 of Polyporus arcularius. The predicted amino acid sequences of Cel1 and Cel2 are similar to glycosyl hydrolase family 7 and 6 proteins, respectively. The expression of cel1 and cel2 was induced by microcrystalline cellulose (Avicel) and cellopentaose but repressed by glucose, cellobiose, cellotriose, and cellotetraose. There was a very low level of cel1 and cel2 transcription regardless of the carbon source. These results suggest that P. arcularius cells constitutively express a very low level of cellulase that can degrade insoluble crystalline cellulose and that the transcription of cel1 and cel2 in the cells is induced by products produced by these endoglucanases such as cellooligosaccharides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Chow CM, Yague E, Raguz S, Wood DA, Thurston CF (1994) The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source. Appl Environ Microbiol 60:2779–2785

    Article  CAS  Google Scholar 

  • Enokibara S, Mori N, Kitamoto Y (1992) Purification and some properties of a carboxymethyl cellulase from Favolus arcularius. J Ferment Bioeng 73:230–232

    Article  CAS  Google Scholar 

  • Gupta R, Jung E, Gooley AA, Williams KL, Brunak S, Hansen J (1999) Scanning the available Dictyostelium discoideum proteome for O-linked GlcNAc glycosylation sites using neural networks. Glycobiology 9:1009–1022

    Article  CAS  Google Scholar 

  • Hamada N, Okumura R, Fuse N, Kodaira R, Shimosaka M, Kanda T, Okazaki M (1999) Isolation and transcriptional analysis of a cellulase gene (cel1) from the basidiomycete Irpex lacteus. J Biosci Bioeng 87:97–102

    Article  CAS  Google Scholar 

  • Henrissat B, Bairoch A (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293:781–788

    Article  CAS  Google Scholar 

  • Henrissat B, Davies G (1997) Structural and sequence-based classification of glycoside hydrolases. Curr Opin Struct Biol 7:637–644

    Article  CAS  Google Scholar 

  • Ilmén M, Saloheimo A, Onnela ML, PenttilaÈ ME (1997) Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol 63:1298–1306

    Article  Google Scholar 

  • Ishihara H, Imamura K, Kita M, Aimi T, Kitamoto Y (2005a) Enhancement of the viscometric endocellulase activity of Polyporus arcularius CMCase IIIa by cellobiose and cellooligosaccharides. Mycoscience 46:148–153

    Article  CAS  Google Scholar 

  • Ishihara H, Aimi T, Takahashi T, Kitamoto Y (2005b) Heterologous expression and characterization of the endocellulase encoding gene cel3A from the basidiomycete Polyporus arcularius. Mycoscience 46:154–161

    Article  CAS  Google Scholar 

  • Jia J, Dyer PS, Buswell JA, Peberdy JF (1999) Cloning of the cbhI and cbhII genes involved in cellulose utilisation by the straw mushroom Volvariella volvacea. Mol Gen Genet 261:985–993

    Article  CAS  Google Scholar 

  • Lee CC, Wong DW, Robertson GH (2001) Cloning and characterization of two cellulase genes from Lentinula edodes. FEMS Microbiol Lett 205:355–360

    Article  CAS  Google Scholar 

  • Mach RL, Zeilinger S (2003) Regulation of gene expression in industrial fungi: Trichoderma. Appl Microbiol Biotechnol 60:515–522

    Article  CAS  Google Scholar 

  • Murai T, Ueda M, Kawaguchi T, Arai M, Tanaka A (1998) Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing beta-glucosidase and carboxymethyl cellulase from Aspergillus aculeatus. Appl Environ Microbiol 64:4857–4861

    Article  CAS  Google Scholar 

  • Nakai K, Kanehisa M (1992) A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics 14:897–911

    Article  CAS  Google Scholar 

  • Novo C, Simoes F, Mendonca D, Matos J, Clemente A (2001) Primary structure deduction and molecular modelling from a cDNA of a cellobiohydrolase-like protein from the white-rot fungus Coriolus versicolor. Int J Biol Macromol 28:285–292

    Article  CAS  Google Scholar 

  • Okada H, Tada K, Sekiya T, Yokoyama K, Takahashi A, Tohda H, Kumagai H, Morikawa Y (1998) Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414. Appl Environ Microbiol 64:555–563

    Article  CAS  Google Scholar 

  • Teeri TT, Lehtovaara P, Kauppinen S, Salovuori I, Knowles J (1987) Homologous domains in Trichoderma reesei cellulolytic enzymes: gene sequence and expression of cellobiohydrolase II. Gene 51:43–52

    Article  CAS  Google Scholar 

  • Vanden Wymelenberg A, Covert S, Cullen D (1993) Identification of the gene encoding the major cellobiohydrolase of the white rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 59:3492–3494

    Article  CAS  Google Scholar 

  • Wood BE, Ingram LO (1992) Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. Appl Environ Microbiol 58:2103–2110

    Article  CAS  Google Scholar 

  • Yague E, Wood DA, Thurston CF (1994) Regulation of transcription of the cel1 gene in Agaricus bisporus. Mol Microbiol 12:41–47

    Article  CAS  Google Scholar 

  • Yague E, Mehak-Zunic M, Morgan L, Wood DA, Thurston CF (1997) Expression of CEL2 and CEL4, two proteins from Agaricus bisporus with similarity to fungal cellobiohydrolase I and beta-mannanase, respectively, is regulated by the carbon source. Microbiology 143:239–244

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Tadanori Aimi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohnishi, Y., Nagase, M., Ichiyanagi, T. et al. Transcriptional regulation of two cellobiohydrolase encoding genes (cel1 and cel2) from the wood-degrading basidiomycete Polyporus arcularius . Appl Microbiol Biotechnol 76, 1069–1078 (2007). https://doi.org/10.1007/s00253-007-1090-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-007-1090-x

Keywords

Navigation