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Production and characterization of medium-chain-length polyhydroxyalkanoate with high 3-hydroxytetradecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442

  • Applied Microbial and Cell Physiology
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Abstract

Medium-chain-length polyhydroxyalkanoates (mcl-PHA) consisting of 3-hydroxyhexanoate (HHx), 3-hydroxyoctanoate (HO), 3-hydroxydecanoate, 3-hydroxydodecanoate, and high-content 3-hydroxytetradecanoate (HTD) was produced by knockout mutant Pseudomonas putida KT2442 termed P. putida KTOY06. When grown on 6 to14 g/L single-carbon-source tetradecanoic acid, P. putida KTOY06, which β-oxidation pathway was weakened by deleting genes of 3-ketoacyl-coenzyme A (CoA) thiolase (fadA) and 3-hydroxyacyl-CoA dehydrogenase (fadB), for the first time, produced several mcl-PHA including 31 to 49 mol% HTD as a major monomer. HHx contents in these mcl-PHAs remained approximately constant at less than 3 mol%. In addition, large amounts of oligo-HTD were detected in cells, indicating the limited ability of P. putida KTOY06 in polymerizing long-chain-length 3-hydroxyalkanoates. The mcl-PHA containing high HTD monomer contents was found to have both higher crystallinity and improved tensile strength compared with that of typical mcl-PHA.

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Acknowledgments

The strain P. putida KTOY06 was kindly donated by Dr. Ouyang SP. We are grateful to Li Ka Shing Foundation and State High Tech Project 863 Grant no. 2006AA02Z242 for financial support. We also like to thank Mr. Luo RC and Wu LP in the Multidisciplinary Research Center of Shantou University for their DSC and MRI studies.

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Authors and Affiliations

  1. Multidisciplinary Research Center, Shantou University, Shantou, 515063, China

    Wenkuan Liu & Guo-Qiang Chen

  2. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China

    Guo-Qiang Chen

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  1. Wenkuan Liu
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  2. Guo-Qiang Chen
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Correspondence to Guo-Qiang Chen.

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Liu, W., Chen, GQ. Production and characterization of medium-chain-length polyhydroxyalkanoate with high 3-hydroxytetradecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442. Appl Microbiol Biotechnol 76, 1153–1159 (2007). https://doi.org/10.1007/s00253-007-1092-8

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  • DOI: https://doi.org/10.1007/s00253-007-1092-8

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