Abstract
The yeast Saccharomyces cerevisiae synthesises a variety of volatile aroma compounds during wine fermentation. In this study, the influence of fermentation temperature on (1) the production of yeast-derived aroma compounds and (2) the expression of genes involved in aroma compounds’ metabolism (ADH1, PDC1, BAT1, BAT2, LEU2, ILV2, ATF1, ATF2, EHT1 and IAH1) was assessed, during the fermentation of a defined must at 15 and 28°C. Higher concentrations of compounds related to fresh and fruity aromas were found at 15°C, while higher concentrations of flowery related aroma compounds were found at 28°C. The formation rates of volatile aroma compounds varied according to growth stage. In addition, linear correlations between the increases in concentration of higher alcohol and their corresponding acetates were obtained. Genes presented different expression profiles at both temperatures, except ILV2, and those involved in common pathways were co-expressed (ADH1, PDC1 and BAT2; and ATF1, EHT1 and IAH1). These results demonstrate that the fermentation temperature plays an important role in the wine final aroma profile, and is therefore an important control parameter to fine-tune wine quality during winemaking.
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References
Aragon P, Atienza J, Climent MD (1998) Influence of clarification, yeast type, and fermentation temperature on the organic acid and higher alcohols of Malvasia and Muscatel wines. Am J Enol Vitic 49(2):211–219
Beltran G, Novo M, Leberre V, Sokol S, Labourdette D, Guillamon JM, Mas A, Rozes JF, Rozes N (2006) Integration of transcriptomic and metabolic analyses for understanding the global responses of low-temperature winemaking fermentations. FEMS Yeast Res 6(8):1167–1183
Boulton R, Singleton V, Bisson L, Kunkee R (1996) Principles and practices of winemaking. Chapman & Hall, New York
Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169–193
Cordente AG, Swiegers JH, Hegardt FG, Pretorius IS (2007) Modulating aroma compounds during wine fermentation by manipulating carnitine acetyltransferases in Saccharomyces cerevisiae. FEMS Microbiol Lett 267(2):159–66
Delfini C, Cocito C, Bonino M, Schellino R, Gaia P, Baiocchi C (2001) Definitive evidence for the actual contribution of yeast in the transformation of neutral precursors of grape aromas. J Agric Food Chem 49:5397–5408
Dickinson JR, Lanterman MM, Danner DJ, Paerson BM, Sanz P, Harrison SJ, Hewlins JE (1997) A 13C nuclear magnetic resonance investigation of the metabolism of leucine to isoamyl alcohol in Saccharomyces cerevisiae. J Biol Chem 272(43):26871–26878
Dickinson JR, Salgado LE, Hewlins JE (2003) The catabolism of amino acids to long chain and complex alcohols in Saccharomyces cerevisiae. J Biol Chem 278(10):8028–8034
Eden A, Simchen G, Benvenisty N (1996) Two yeast homologs of ECA39, a target for c-Myc regulation, code for cytosolic and mitochondrial branched-chain amino acid aminotransferases. J Biol Chem 271(34):20242–20245
Eden A, Van Nedervelde L, Drukker M, Benvenisty N, Debourg A (2001) Involvement of branched-chain amino acid aminotransferases in the production of fusel alcohols during fermentation in yeast. Appl Microbiol Biotechnol 55:296–300
Étievant PX (1991) Wine. In: Maarse H (ed) Volatile compounds in foods and beverages. 2nd edn. Marcel Dekker, New York, pp 483–546
Fukuda K, Yamamoto N, Kiyokawa Y, Yanagiuchi T, Wakai Y, Kitamoto K, Inoue Y, Kimura A (1998) Balance of activities of alcohol acetyltransferase and esterase in Saccharomyces cerevisiae is important for production of isoamyl acetate. Appl Environ Microbiol 64(10):4076–4078
Gómez-Míguez MJ, Cacho JF, Ferreira V, Vicario IM, Heredia FJ (2007) Volatile components of Zalema white wines. Food Chem 100:1464–1473
Guth H (1997) Quantitation and sensory studies of character impact odorants of different white wine varieties. J Agric Food Chem 45:3027–3032
Killian E, Ough CS (1979) Fermentation esters–formation and retention as affected by fermentation temperature. Am J Enol Vitic 30(4):301–305
Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma - a review. S Afr J Enol Vitic 21(Special Issue):97–129
Lilly M, Lambrechts MG, Pretorius IS (2000) Effect of increased yeast alcohol acetyltransferase activity on flavour profiles of wine and destillates. Appl Environ Microbiol 66:744–753
Lilly M, Bauer FF, Lambrechts MG, Swiegers JH, Cozzolino D, Pretorius IS (2006a) The effect of increased alcohol acetyl transferase and esterase activity on flavour profiles of wine and distillates. Yeast 23:641–659
Lilly M, Styger G, Bauer FF, Lambrechts MG, Pretorius IS (2006b) The effect of increased yeast branched-chain amino acid transaminase activity and the production of higher alcohols on the flavour profiles of wine and distillates. FEMS Yeast Res 6:726–743
Masneuf-Pomarède I, Mansour C, Murat M, Tominaga T, Dubourdieu D (2006) Influence of fermentation temperature on volatile thiols concentrations in Sauvignon blanc wines. Int J Food Microbiol 108:385–390
Mason AB, Dufour J (2000) Alcohol acetyltransferases and the significance of ester synthesis in yeast. Yeast 16:1287–1298
Mauricio JC, Moreno JJ, Valero EM, Zea L, Medina M, Ortega JM (1993) Ester formation and specific activities of in vitro alcohol acetyltransferase and esterase by Saccharomyces cerevisiae during grape must fermentation. J Agric Food Chem 41:2086–2091
Novo M, Beltran G, Rozes N, Guillamon JM, Sokol S, Leberre V, Francois J, Mas A (2006) Early transcriptional response of wine yeast after rehydration: osmotic shock and metabolic activation. FEMS Yeast Res 7(2):304–316
Riou C, Nicaud JM, Barre P, Gaillardin C (1997) Stationary-phase gene expression in Saccharomyces cerevisiae during wine fermentation. Yeast 13:903–915
Rossignol T, Dulau L, Julien A, Blondin B (2003) Genome-wide monitoring of wine yeast gene expression during alcoholic fermentation. Yeast 20(16):1369–1385
Saerens SM, Verstrepen KJ, Van Laere SDM, Voet AR, Van Dijck P, Delvaux FR, Thevelein JM (2006) The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity. J Biol Chem 281(7):4446–4456
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor, New York
Siebert TE, Smyth HE, Capone DL, Neuwöhner C, Pardon KH, Skouroumounis GK, Herderich MJ, Sefton MA, Pollnitz AP (2005) Stable isotope dilution analysis of wine fermentation products by HS-SPME-GC-MS. Anal Bioanal Chem 381:937–947
Stashenko H, Macku C, Shibamato T (1992) Monitoring volatile chemicals formed from must during yeast fermentation. J Agric Food Chem 40:2257–2259
Swiegers JH, Pretorius IS (2005) Yeast modulation of wine flavour. Adv Appl Microbiol 57:131–175
Swiegers JH, Bartowksy EJ, Henschke PA, Pretorius IS (2005) Yeast and bacterial modulation of wine aroma and flavour. Aust J Grape Wine Res 11:127–138
Swiegers JH, Francis IL, Herderich MJ, Pretorius IS (2006) Meeting consumer expectations through management in vineyard and winery: the choice of yeast for fermentation offers great potential to adjust the aroma of Sauvignon Blanc wine. Aust N Z Wine Ind J 21:34–42
Teranishi R, Flath RA, Guadagni DG, Lundin RE, Mon TR, Stevens KL (1966) Gas chromatographic, infrared, proton magnetic resonance, mass spectral, and threshold analyses of all pentyl acetates. J Agric Food Chem 14(3):253–262
Torija MJ, Beltran G, Novo M, Poblet M, Guillamón JM, Mas A, Rozès N (2003) Effects of fermentation temperature and Saccharomyces species on the cell fatty acid composition and presence of volatile compounds in wine. Int J Food Microbiol 85:127–136
Varela C, Pizarro F, Agosin E (2004) Biomass content governs fermentation rate in nitrogen-deficient wine musts. Appl Environ Microbiol 70(6):3392–3400
Verstrepen KJ, Van Laere SDM, Vercammen J, Derdelinckx G, Dufour JP, Pretorius IS, Winderickx J, Thevelein JM, Delvaux FR (2004) The Saccharomyces cerevisiae alcohol acetyl transferase Atf1p is localized in lipid particles. Yeast 21:367–377
Vianna E, Ebeler SE (2001) Monitoring ester formation in grape juice fermentations using solid phase microextraction coupled with gas chromatography-mass spectrometry. J Agric Food Chem 49:589–595
Walker G (1998) Yeast physiology and biotechnology. Wiley, New York
Wondra M, Boveric M (2001) Analyses of aroma components of Chardonnay wine fermented by different yeast strains. Food Technol Biotechnol 39(2):141–148
Acknowledgement
This work was supported by (1) Fondo Nacional para el Desarrollo Científico y Tecnológico de Chile (FONDECYT) grant 1050688, (2) Programa de Mejoramiento de la Calidad y la Equidad de la Educación Superior (MECESUP) PUC 9902/9903, (3) 2005 Endeavour Australia Postgraduate and Postdoctoral Research Fellowships (Department of Education, Science and Training, Australian Government), and by (4) Australia’s grapegrowers and winemakers through their investment body the Grape and Wine Research Development Corporation with matching funding from the Australian Government.
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Molina, A.M., Swiegers, J.H., Varela, C. et al. Influence of wine fermentation temperature on the synthesis of yeast-derived volatile aroma compounds. Appl Microbiol Biotechnol 77, 675–687 (2007). https://doi.org/10.1007/s00253-007-1194-3
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DOI: https://doi.org/10.1007/s00253-007-1194-3