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An exopolysaccharide produced by the novel halophilic bacterium Halomonas stenophila strain B100 selectively induces apoptosis in human T leukaemia cells

  • Applied Genetics and Molecular Biotechnology
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Abstract

Microbial exopolysaccharides (EPSs) are highly heterogeneous polymers produced by fungi and bacteria and have recently been attracting considerable attention from biotechnologists because of their potential applications in many fields, including biomedicine. We have screened the antitumoural activity of a panel of sulphated EPSs produced by a newly discovered species of halophilic bacteria. We found that the novel halophilic bacterium Halomonas stenophila strain B100 produced a heteropolysaccharide that, when oversulphated, exerted antitumoural activity on T cell lines deriving from acute lymphoblastic leukaemia (ALL). Only tumour cells were susceptible to apoptosis induced by the sulphated EPS (B100S), whilst primary T cells were resistant. Moreover, freshly isolated primary cells from the blood of patients with ALL were also susceptible to B100S-induced apoptosis. The newly discovered B100S is therefore the first bacterial EPS that has been demonstrated to exert a potent and selective pro-apoptotic effect on T leukaemia cells, and thus, we propose that the search for new antineoplastic drugs should include the screening of other bacterial EPSs, particularly those isolated from halophiles.

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Acknowledgements

We are grateful to our colleagues Dr. Francisco Ruiz-Cabello for providing leukaemia blood samples. We acknowledge the generous supply of rIL-2 provided by the National Institutes of Health AIDS Reference and Reagent Program (Rockville, MD). This research was funded by Grants SAF2006-06246 and SAF2009/09818 from the Ministry of Innovation and Science of Spain and P06-CTS-02112 from the Department of Innovation and Science, Regional Government of Andalucia to IJM.

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Authors and Affiliations

  1. Institute of Biopathology and Regenerative Medicine, Centre for Biomedical Research, University of Granada Health-Sciences Technology Park, Avda. del Conocimiento s/n, 18100, Armilla-Granada, Spain

    Carmen Ruiz-Ruiz, Girish K. Srivastava, Diana Carranza & Ignacio J. Molina

  2. Department of Microbiology, Faculty of Pharmacy, University of Granada, Granada, Spain

    Juan A. Mata, Inmaculada Llamas & Emilia Quesada

  3. Department of Immunology, Faculty of Medicine, University of Cordoba, Cordoba, Spain

    Manuel Santamaría

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  1. Carmen Ruiz-Ruiz
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  2. Girish K. Srivastava
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  3. Diana Carranza
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  4. Juan A. Mata
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  5. Inmaculada Llamas
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  8. Ignacio J. Molina
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Correspondence to Ignacio J. Molina.

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Ruiz-Ruiz, C., Srivastava, G.K., Carranza, D. et al. An exopolysaccharide produced by the novel halophilic bacterium Halomonas stenophila strain B100 selectively induces apoptosis in human T leukaemia cells. Appl Microbiol Biotechnol 89, 345–355 (2011). https://doi.org/10.1007/s00253-010-2886-7

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