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Development of an oral mucosa model to study host-microbiome interactions during wound healing

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Abstract

Crosstalk between the human host and its microbiota is reported to influence various diseases such as mucositis. Fundamental research in this area is however complicated by the time frame restrictions during which host-microbe interactions can be studied in vitro. The model proposed in this paper, consisting of an oral epithelium and biofilm, can be used to study microbe-host crosstalk in vitro in non-infectious conditions up to 72 h. Microbiota derived from oral swabs were cultured on an agar/mucin layer and challenged with monolayers of keratinocytes grown on plastic or collagen type I layers embedded with fibroblasts. The overall microbial biofilm composition in terms of diversity remained representative for the oral microbiome, whilst the epithelial cell morphology and viability were unaffected. Applying the model to investigate wound healing revealed a reduced healing of 30 % in the presence of microbiota, which was not caused by a reduction of the proliferation index (52.1–61.5) or a significantly increased number of apoptotic (1–1.13) or necrotic (32–30.5 %) cells. Since the model allows the separate study of the microbial and cellular exometabolome, the biofilm and epithelial characteristics after co-culturing, it is applicable for investigations within fundamental research and for the discovery and development of agents that promote wound healing.

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Acknowledgments

Tine De Ryck is a doctoral research fellow supported by the FWO—Flanders (G.0712.10 N). Tom Van de Wiele is financially supported by FWO (1526012 N) and GOA (BOF12/GOA/008). Barbara Vanhoecke’s research leading to these results has received funding from GOA (BOF 01G01307) and the Seventh Framework Programme (FP7/2011) under grant agreement no. 299169.

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There are no conflicts of interest to declare.

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Authors and Affiliations

  1. Laboratory of Experimental Cancer Research (LECR), Ghent University, De Pintelaan 185 1P7, B-9000, Ghent, Belgium

    Tine De Ryck, Marc Bracke & Barbara Vanhoecke

  2. Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000, Ghent, Belgium

    Charlotte Grootaert, Laura Jaspaert, Frederiek-Maarten Kerckhof, Pieter Van den Abbeele, Tom Van de Wiele & Barbara Vanhoecke

  3. Laboratory of Food Chemistry and Human Nutrition, Ghent University, B-9000, Ghent, Belgium

    Charlotte Grootaert

  4. Dermatology Department, University Hospital Ghent, B-9000, Ghent, Belgium

    Mireille Van Gele

  5. Laboratory of Bioinformatics and Computational Genomics (Biobix), Ghent University, B-9000, Ghent, Belgium

    Joachim De Schrijver

  6. Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland, New Zealand

    Simon Swift

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  1. Tine De Ryck
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Correspondence to Tine De Ryck or Tom Van de Wiele.

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De Ryck, T., Grootaert, C., Jaspaert, L. et al. Development of an oral mucosa model to study host-microbiome interactions during wound healing. Appl Microbiol Biotechnol 98, 6831–6846 (2014). https://doi.org/10.1007/s00253-014-5841-1

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  • DOI: https://doi.org/10.1007/s00253-014-5841-1

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